MG_205_DIMER – heat-inducible transcription repressor HrcA, putative

Name
WID	MG_205_DIMER
Name	heat-inducible transcription repressor HrcA, putative

Structure
Biosynthesis	[c]: (2) MG_205_MONOMER ⇒ MG_205_DIMER
No. subunits	2
DNA footprint	Length: 33 (nt), Binding: dsDNA, Region: dsDNA
Empirical formula (pH 7.5)	H₅₈₃₆C₃₅₉₈N₉₃₈O₁₀₃₂S₁₀
Molecular weight (pH 7.5; Da)	79066.92
Extinction coefficient (260 nm, 25C, pH 7.0)	-49990.00
Half life (OD (600 nm) = 0.3, M9 media, 36C; min)	1200.00

Synthesis
Formation process	Process_MacromolecularComplexation
Localization	c

Regulation
Regulatory rule	temperature > 43

Function
Transcriptional regulation	TranscriptionalRegulation_hrcA_TU_010: TU_010 TranscriptionalRegulation_hrcA_TU_122: TU_122 TranscriptionalRegulation_hrcA_TU_155: TU_155 TranscriptionalRegulation_hrcA_TU_208: TU_208 TranscriptionalRegulation_hrcA_TU_258: TU_258 TranscriptionalRegulation_hrcA_TU_285: TU_285
Reaction participant	DNA bound protein release (Holliday junction DNA helicase releases heat-inducible transcription repressor HrcA, putative) MG_205_DIMER[d] + MG_358_359_10MER[c] ⇒ MG_205_DIMER[c] + MG_358_359_10MER[d] DNA bound protein release (replicative DNA helicase releases heat-inducible transcription repressor HrcA, putative) MG_094_HEXAMER[c] + MG_205_DIMER[d] ⇒ MG_094_HEXAMER[d] + MG_205_DIMER[c] DNA bound protein release (heat-inducible transcription repressor HrcA, putative releases DNA topoisomerase I) MG_122_MONOMER[d] + MG_205_DIMER[c] ⇒ MG_122_MONOMER[c] + MG_205_DIMER[d] DNA bound protein release (heat-inducible transcription repressor HrcA, putative releases DnaA-ADP) MG_205_DIMER[c] + MG_469_1MER_ADP[d] ⇒ MG_205_DIMER[d] + MG_469_1MER_ADP[c] DNA bound protein release (type I restriction modification DNA specificity domain protein releases heat-inducible transcription repressor HrcA, putative) MG_205_DIMER[d] + MG_438_MONOMER[c] ⇒ MG_205_DIMER[c] + MG_438_MONOMER[d] ... 22 more DNA bound protein release (DNA polymerase (2) core, β-clamp, γ-complex, and primase releases heat-inducible transcription repressor HrcA, putative) DNA_POLYMERASE_2CORE_BETA_CLAMP_GAMMA_COMPLEX_PRIMASE[c] + MG_205_DIMER[d] ⇒ DNA_POLYMERASE_2CORE_BETA_CLAMP_GAMMA_COMPLEX_PRIMASE[d] + MG_205_DIMER[c] DNA bound protein release (DNA polymerase III, beta clamp releases heat-inducible transcription repressor HrcA, putative) MG_001_DIMER[c] + MG_205_DIMER[d] ⇒ MG_001_DIMER[d] + MG_205_DIMER[c] DNA bound protein release (DNA ligase, NAD-dependent releases heat-inducible transcription repressor HrcA, putative) MG_205_DIMER[d] + MG_254_MONOMER[c] ⇒ MG_205_DIMER[c] + MG_254_MONOMER[d] DNA bound protein release (heat-inducible transcription repressor HrcA, putative releases Chromosome Segregation Protein SMC with SCP Proteins-ADP) MG_205_DIMER[c] + MG_213_214_298_6MER_ADP[d] ⇒ MG_205_DIMER[d] + MG_213_214_298_6MER_ADP[c] DNA bound protein release (heat-inducible transcription repressor HrcA, putative releases DNA gyrase) DNA_GYRASE[d] + MG_205_DIMER[c] ⇒ DNA_GYRASE[c] + MG_205_DIMER[d] DNA bound protein release (polI-like 5'-3' exonuclease, putative releases heat-inducible transcription repressor HrcA, putative) MG_205_DIMER[d] + MG_262_MONOMER[c] ⇒ MG_205_DIMER[c] + MG_262_MONOMER[d] DNA bound protein release (heat-inducible transcription repressor HrcA, putative releases topoisomerase IV) MG_203_204_TETRAMER[d] + MG_205_DIMER[c] ⇒ MG_203_204_TETRAMER[c] + MG_205_DIMER[d] DNA bound protein release (DNA polymerase core, β-clamp, and primase releases heat-inducible transcription repressor HrcA, putative) DNA_POLYMERASE_CORE_BETA_CLAMP_PRIMASE[c] + MG_205_DIMER[d] ⇒ DNA_POLYMERASE_CORE_BETA_CLAMP_PRIMASE[d] + MG_205_DIMER[c] DNA bound protein release (Holliday junction endonuclease releases heat-inducible transcription repressor HrcA, putative) MG_205_DIMER[d] + MG_352_DIMER[c] ⇒ MG_205_DIMER[c] + MG_352_DIMER[d] DNA bound protein release (DNA-directed RNA polymerase releases heat-inducible transcription repressor HrcA, putative) MG_205_DIMER[d] + RNA_POLYMERASE[c] ⇒ MG_205_DIMER[c] + RNA_POLYMERASE[d] DNA bound protein release (DNA polymerase core, β-clamp, and γ-complex releases heat-inducible transcription repressor HrcA, putative) DNA_POLYMERASE_CORE_BETA_CLAMP_GAMMA_COMPLEX[c] + MG_205_DIMER[d] ⇒ DNA_POLYMERASE_CORE_BETA_CLAMP_GAMMA_COMPLEX[d] + MG_205_DIMER[c] DNA bound protein release (adenine-specific DNA modification methylase releases heat-inducible transcription repressor HrcA, putative) MG_184_DIMER[c] + MG_205_DIMER[d] ⇒ MG_184_DIMER[d] + MG_205_DIMER[c] DNA bound protein release (heat-inducible transcription repressor HrcA, putative releases DnaA-ATP) MG_205_DIMER[c] + MG_469_1MER_ATP[d] ⇒ MG_205_DIMER[d] + MG_469_1MER_ATP[c] DNA bound protein release (uracil-DNA glycosylase, putative releases heat-inducible transcription repressor HrcA, putative) MG_097_MONOMER[c] + MG_205_DIMER[d] ⇒ MG_097_MONOMER[d] + MG_205_DIMER[c] DNA bound protein release (DNA polymerase III, beta subunit releases heat-inducible transcription repressor HrcA, putative) MG_001_MONOMER[c] + MG_205_DIMER[d] ⇒ MG_001_MONOMER[d] + MG_205_DIMER[c] DNA bound protein release (recombination protein, strand exchange releases heat-inducible transcription repressor HrcA, putative) MG_205_DIMER[d] + MG_339_MONOMER[c] ⇒ MG_205_DIMER[c] + MG_339_MONOMER[d] DNA bound protein release (apurinic endonuclease releases heat-inducible transcription repressor HrcA, putative) MG_205_DIMER[d] + MG_235_MONOMER[c] ⇒ MG_205_DIMER[c] + MG_235_MONOMER[d] DNA bound protein release (DNA-directed RNA polymerase holoenzyme releases heat-inducible transcription repressor HrcA, putative) MG_205_DIMER[d] + RNA_POLYMERASE_HOLOENZYME[c] ⇒ MG_205_DIMER[c] + RNA_POLYMERASE_HOLOENZYME[d] DNA bound protein release (3-5' helicase releases heat-inducible transcription repressor HrcA, putative) MG_205_DIMER[d] + MG_244_DIMER[c] ⇒ MG_205_DIMER[c] + MG_244_DIMER[d] DNA bound protein release (formamidopyrimidine-DNA glycosylase releases heat-inducible transcription repressor HrcA, putative) MG_205_DIMER[d] + MG_498_MONOMER[c] ⇒ MG_205_DIMER[c] + MG_498_MONOMER[d] DNA bound protein release (DNA incision complex releases heat-inducible transcription repressor HrcA, putative) MG_073_206_421_TETRAMER[c] + MG_205_DIMER[d] ⇒ MG_073_206_421_TETRAMER[d] + MG_205_DIMER[c] DNA bound protein release (putative DNA integrity scanning protein releases heat-inducible transcription repressor HrcA, putative) MG_105_OCTAMER[c] + MG_205_DIMER[d] ⇒ MG_105_OCTAMER[d] + MG_205_DIMER[c]
Complex subunit	heat-inducible transcription repressor HrcA, putative [c]: (2) MG_205_MONOMER ⇒ MG_205_DIMER

Comments
Comments	Composition [PUB_0206]. Transcriptional Regulation Consensus CIRCE sequence TTAGCACTC---------GAGTGCTAA is 27 bp [PUB_0186, PUB_0418, PUB_0419, PUB_0433]. Footprint extends 5-6 nt beyond CIRCE inverted repeats [PUB_0723]. Regulation: Heat represses HrcA transription factor leading to derepression of heat-shock proteins including chaperones and chaperonins [PUB_0186, PUB_0418, PUB_0419, PUB_0433].
References	Güell M, van Noort V, Yus E, Chen WH, Leigh-Bell J, Michalodimitrakis K, Yamada T, Arumugam M, Doerks T, Kühner S, Rode M, Suyama M, Schmidt S, Gavin AC, Bork P, Serrano L. Transcriptome complexity in a genome-reduced bacterium. Science 326, 1268-71 (2009). WholeCell: PUB_0418, PubMed: 19965477 Kwon HY, Kim EH, Tran TD, Pyo SN, Rhee DK. Reduction-sensitive and cysteine residue-mediated Streptococcus pneumoniae HrcA oligomerization in vitro. Mol Cells 27, 149-57 (2009). WholeCell: PUB_0206, PubMed: 19277496 Musatovova O, Dhandayuthapani S, Baseman JB. Transcriptional heat shock response in the smallest known self-replicating cell, Mycoplasma genitalium. J Bacteriol 188, 2845-55 (2006). WholeCell: PUB_0186, PubMed: 16585746 Sierro N, Makita Y, de Hoon M, Nakai K. DBTBS: a database of transcriptional regulation in Bacillus subtilis containing upstream intergenic conservation information. Nucleic Acids Res 36, D93-6 (2008). WholeCell: PUB_0419, PubMed: 17962296, URL: http://dbtbs.hgc.jp/ Wilson AC, Tan M. Stress response gene regulation in Chlamydia is dependent on HrcA-CIRCE interactions. J Bacteriol 186, 3384-91 (2004). WholeCell: PUB_0723, PubMed: 15150223 Zuber U, Schumann W. CIRCE, a novel heat shock element involved in regulation of heat shock operon dnaK of Bacillus subtilis. J Bacteriol 176, 1359-63 (1994). WholeCell: PUB_0433, PubMed: 8113175

Metadata
Created	2012-10-01 15:07:38
Last updated	2012-10-01 15:14:08