MG_205 – heat-inducible transcription repressor HrcA, putative

Name
WID	MG_205
Name	heat-inducible transcription repressor HrcA, putative
Symbol	hrcA
Protein product	MG_205_MONOMER
Cross references	GenBank: AAC71423.1, CMR: MG_205, BioCyc: MG_205, SwissProt: P47447
Homologs	B. subtilis: BSU25490 M. hyopneumoniae: mhp010 M. mobile: mhp010 M. pneumoniae: MPN_124 S. coelicolor: SCO2555

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 244566 (nt), Length: 1032 (nt), Direction: Reverse, G/C content: 34.5%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 ATGAAGAATTTAACGCCACGGCAAGCCCAAATTCTCAAAGCAATTATCAA TGAATACATTGCTTATGCTATCCCTGTTGGCAGTAAATTACTAACCAAAA AATATTTTAAAAACCTCTCTGGGGGAACATTACGAAATGAGATGGCAGCT TTAGAGAAAAAAGGGTTCTTAAAGAAAAACCACATCTCTTCAGGCCGTGT TCCTTCCCAAATTGGTTATCAGTATTATGTCAAAGTGTTAAATGTTAGTA ACACCACTAACGATCTGAAAACCCGCTTAAGAAGTGTTATTCTCCAGCAA CACAAAACGATTGATGAGGTGATTGAACTTGGGGTTAAGTTTATCAATGA GATCATTAACCTACCAGTAGTGTTAACTAACTTTAGTAGTGATGAAGTTT TAAAAAAGATTGATCTGATCATCCTTGATAAGTCATTTGCCTTATTTTTA TTGGTCTCCGCTAGTGGTAAAGTCTTTAAAAAAACCATTAGCTATGCTAA CCAAAGGCAGTTTGAAGACATTGTTATTTGTGTAAGGATCTTTAATGATC GCATCATTGATACCCGCTTTTCAGAGATTAACAACCAACTAGAAGTTTTA AAAGAGATCATTAGAACCAAGGTTCATGAGTATCAGTATGTAATTGATGA GATCTTATTTAAGTTATTTGATTTAGATCAAATTGAGGCAAATAAAAAGA TCTATGGGATCCAATACCTTGCTAAACAACCCGAGTTTGCTAACCAAGAG AAACTCACTAAAATCTTAAATCTGCTTGAAGATACTAGTGTTTGACAACA GATGGCATTCATAAACCAAACCAACCAAAAGACCAACATTGTCTTTGGTG ATCAATTGGGTTTTAAAGAGATATCAGTTGCTTCTACTTTAATCAATACC ACCAGTGAAGCCAAGCACCAACTTGCTATAGTAGGACCAACCAGAATGGA CTACCAAAAGATTAAAGCCTTATTAACTACTCTAAAAGAAGAGATAGAAA AATATGATAAAAAGATCCACAACCAAACCTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1032
Transcription unit	TU_125
Empirical formula (pH 7.5)	H₁₁₀₅₆C₁₀₀₂₃N₄₂₆₉O₇₁₄₃P₁₀₃₂
Molecular weight (pH 7.5; Da)	337569.87

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.851454 (dimensionless) (Show evidence) Value: 0.851454 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	10629000
pI	3.78

Comments
Comments	oligomer represses transcription of promoters with CIRCE inverted repeat [PUB_0204, PUB_0206]; CIRCE sequences: 5'-aaaatatttt-N(9)-aaactaattt-3', 4 nt from start codon of regulated genes [PUB_0207]; positively regulated by groEL [PUB_0205]; reducing agents, heat promote dissociation of oligomers to monomers [PUB_0206]; only active as repressor in dimer form [PUB_0206]; present most as tetramer -- dimer 6%, tetramer 50%, octamer 31%, hexadecamer 5% [PUB_0206]; found in membrane fraction, may an integral membrane protein [PUB_0206]; acts as a reductionoxidation sensor via a Ca2+-induced conformational rearrangement resulting in oligomerization [PUB_0206];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Chang LJ, Chen WH, Minion FC, Shiuan D. Mycoplasmas regulate the expression of heat-shock protein genes through CIRCE-HrcA interactions. Biochem Biophys Res Commun 367, 213-8 (2008). WholeCell: PUB_0207, PubMed: 18164681 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Kwon HY, Kim EH, Tran TD, Pyo SN, Rhee DK. Reduction-sensitive and cysteine residue-mediated Streptococcus pneumoniae HrcA oligomerization in vitro. Mol Cells 27, 149-57 (2009). WholeCell: PUB_0206, PubMed: 19277496 Lemos JA, Chen YY, Burne RA. Genetic and physiologic analysis of the groE operon and role of the HrcA repressor in stress gene regulation and acid tolerance in Streptococcus mutans. J Bacteriol 183, 6074-84 (2001). WholeCell: PUB_0205, PubMed: 11567008 ... 2 more Segal G, Ron EZ. Regulation of heat-shock response in bacteria. Ann N Y Acad Sci 851, 147-51 (1998). WholeCell: PUB_0204, PubMed: 9668617 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:19
Last updated	2012-10-01 15:13:08