MG_190 – phosphoesterase

Name
WID	MG_190
Name	phosphoesterase
Symbol	mgpA
Synonyms	recJ
Protein product	MG_190_MONOMER
Cross references	GenBank: AAC71409.1, CMR: MG_190, BioCyc: MG_190, SwissProt: P22746
Homologs	M. hyopneumoniae: mhp006 M. mobile: mhp006 M. pneumoniae: MPN_140

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 220607 (nt), Length: 957 (nt), Direction: Forward, G/C content: 33.5%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 ATGAAAAAAGGATCAATAACTGAAGCAATTAATGCCATTAAACAATTTGA TAAGATTGTTATCTTTCACCATGTGCGCCCTGATGGGGATTGTTTAGGAG CACAACAAGGCTTGTTTCACCTCATTAAAGCTAACTTTAAAAATAAGGAG GTGAAGTGTGTTGGTAATAACAACAACCTGTTTAGCTTTATCAACATGAC ATTTACCAACCAAATTGATGAGAGCTTTTTAAAAGAAGCACTTGCCATTG TGGTCGATGCTAATTACAAAAACAGGATTGAATTGAGAGAACTGTTAGAT AAAAACCTGTTTAAAGCAGTGTTAAGGATTGATCACCATCCCAATGAAGA TGATCTAAACACTAGCTTTAACTTTGTTGAAGAAAGCTATGTAGCTTGTT GTGAGCAGATAGTGGAGATGGCCACAGTGGCGAAGTGGACCATACCACCA GTGGCTGCTACTTTACTATATATAGGTATCTATACGGATAGTAATAGATT TCTATATAGTAATACATCATATAGAACACTATACTTAGCAGCAATACTAT ATAAAGCTAAAGCTGATATAAGGATAGTACATGATCATTTAAACCATACT AGTTTAGCAGATCTTAAGTTTAAAAAGTATGTTTATAACCACTTTAAAAC CCAAGGACAAGTGATCTATTTTATCTGTACTAAAAAGATCCAAAAGAGAC TAAGAATGACTGCAGATCAATGTGCTAGAGTTAACTTGTTAAGTAACATA GCAGATTACAAGATCTGACTTTTCTTTATTGAACAAGCTAATAATGAGAT CAGGATAGAACTGAGGAGTAATGGGATTAATGTCAGAGATATAGCCATTA AGTATGGTGGGGGAGGACATAATAATGCAAGTGGAGCGATCATTACTAAC AAAAAACAAATTAGTGATGTTGTTAGTGATTGTGTGAAAAAAATTGTTTA TAATTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 957
Transcription unit	TU_117
Empirical formula (pH 7.5)	H₁₀₂₄₇C₉₂₈₉N₃₉₄₂O₆₆₂₆P₉₅₇
Molecular weight (pH 7.5; Da)	312763.71

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	1.29325 (dimensionless) (Show evidence) Value: 1.29325 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	9997300
pI	3.83

Comments
Comments	Introduction MG_190 codes for DHH phosphoesterase family protein with weak homology to 5-3' ssDNA exonuclease recJ [PUB_0110]. Carvalho et al concluded in 2005 that there is no recJ in M. genitalium [PUB_0072]. recJ Processive exonuclease, degrading ~1000 nt after a single binding event [PUB_0148]. DNA binding and nuclease activity of RecJ was specifically enhanced by the pre-addition of ssDNA-binding protein [PUB_0148]. Needs at least 7 ss nucleotides to bind [PUB_0148]. Exonuclease activity can be terminated by ds region [PUB_0148]. Releases mononucleotides [PUB_0148]. Possibly has exonuclease activity required to produce a free 3' single-stranded DNA to which RecA can bind for the initiation of homologous recombination [PUB_0147]. Exonuclease activity requires Mg2+, Mn2+, or Co2+ [PUB_0552]. Yamagata et al observed the exonuclease kinetics for the complete protein: kcat=2×10-3 1/s, Km=4.1 μM, and for a truncated protein: kcat>130 1/s, Km=6.1 μM [PUB_0552].
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Carvalho FM, Fonseca MM, Batistuzzo De Medeiros S, Scortecci KC, Blaha CA, Agnez-Lima LF. DNA repair in reduced genome: the Mycoplasma model. Gene 360, 111-9 (2005). WholeCell: PUB_0072, PubMed: 16153783 Chen J, Anderson JB, DeWeese-Scott C, Fedorova ND, Geer LY, He S, Hurwitz DI, Jackson JD, Jacobs AR, Lanczycki CJ, Liebert CA, Liu C, Madej T, Marchler-Bauer A, Marchler GH, Mazumder R, Nikolskaya AN, Rao BS, Panchenko AR, Shoemaker BA, Simonyan V, Song JS, Thiessen PA, Vasudevan S, Wang Y, Yamashita RA, Yin JJ, Bryant SH. MMDB: Entrez's 3D-structure database. Nucleic Acids Res 31, 474-7 (2003). WholeCell: PUB_0110, PubMed: 12520055, URL: http://www.ncbi.nlm.nih.gov/Structure/ Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Han ES, Cooper DL, Persky NS, Sutera VA Jr, Whitaker RD, Montello ML, Lovett ST. RecJ exonuclease: substrates, products and interaction with SSB. Nucleic Acids Res 34, 1084-91 (2006). WholeCell: PUB_0148, PubMed: 16488881 ... 3 more Iverson-Cabral SL, Astete SG, Cohen CR, Rocha EP, Totten PA. Intrastrain heterogeneity of the mgpB gene in Mycoplasma genitalium is extensive in vitro and in vivo and suggests that variation is generated via recombination with repetitive chromosomal sequences. Infect Immun 74, 3715-26 (2006). WholeCell: PUB_0147, PubMed: 16790744 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319 Yamagata A, Masui R, Kakuta Y, Kuramitsu S, Fukuyama K. Overexpression, purification and characterization of RecJ protein from Thermus thermophilus HB8 and its core domain. Nucleic Acids Res 29, 4617-24 (2001). WholeCell: PUB_0552, PubMed: 11713311

Metadata
Created	2012-10-01 15:07:18
Last updated	2012-10-01 15:13:06