MG_187 – Oligosaccharide or polyol ABC transporter, ATP-binding protein

Name
WID	MG_187
Name	Oligosaccharide or polyol ABC transporter, ATP-binding protein
Protein product	MG_187_MONOMER
Cross references	GenBank: AAC71406.1, CMR: MG_187, BioCyc: MG_187, SwissProt: P47433
Homologs	B. subtilis: BSU38810 M. hyopneumoniae: mhp380 M. mobile: mhp380 M. pneumoniae: MPN_134

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 216761 (nt), Length: 1758 (nt), Direction: Forward, G/C content: 33.8%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151 1201 1251 1301 1351 1401 1451 1501 1551 1601 1651 1701 1751 ATGGCTAACAATAAGAGTGCAATTGAGTTGAAAAACATCGTTGTTGATTT TGGTGAATCAGTTGCGATTGACAACATTAACCTTAGTGTTGAAAAACACC AACTAGTTAGCTTACTTGGTCCTAGTGGTTGTGGTAAAACCACTACACTT GCAGTTATTGCAGGACTTATTAAACCAACTAGTGGTCAGGTGTTATTTAA TGGTTATGATGTCACCAAAAAACCACCCCAAGAACGTAAACTAGGGCTAG TTTTTCAAAACTATGCACTTTATCCGCACATGAATGTGTTTGAAAACATT GTTTTCCCCCTCTACAGTGATAACTCGTGAAAACAAGCAGTTTTGGAAAA AAACAGTGTTGCAAACCATGAGATTAACTGTTTGTTACTTACTAGCAACG GTGCATCAGTTCAAGAGATTGATCAGCTCAATAAGTTATTTCATGATAGT ATTGAAAAACCCAAACAGATCCAATACCAAATTAATGACCTTAATGTTAG TGTTTTTAAAAACTTAAATGAACTAACTGCAAACCTTAAGTTAATACCAA GTAAGCACCAGTTTGCTATTACCAATCTCAACAAACAAACTCTAAAACAG ATTAATGAACTGGAAGCTGAGTTTAAAACAAAGTGAAAGTTACAAAAACA AACCCCAATTAAGAGTGGGGTTGAACACAATGCCAAACTCCAAGCAATTA AACAACACTTTAGTTATGAAAAACAACGGTTAAAAAAACACTATTTCAAA ACTAAAGTGGAACTAAAACAAACCCTTGTTGAAAACCTTAAGTTAGTTAA AAAAGCGATTAGTGAACAAACTAAGTTAATTAAACAGAGTAGTGATTACA CTAAGTTAAAGCAATTAAAACGGTTAATTAAAGTTGAACCTAACCAACTC AAAAAACAATATAAGGTTTTTCTCAATCAGTTAATTAAAAACTATTCACT TAAAACTGATAAGTTAACTGATACTCAACTTAATGAAATTGAACAGATTA AAACCAGAATTGTTTCAATAAAACAGTTTATCAACAAAACTGCACTTGAA GTAGCTAACAAACTAGCGATTACCAAGATTTTAACCAAACGCCCTGATAA GATTTCTGGTGGACAACAACAACGCGTAGCAATTGCTAGAGCAATTGTCA GAAGACCTAAACTATTGTTAATGGATGAACCACTCTCTAACTTAGATGCA AAGCTAAGGGTACAGACAAGACAGTGGATCAGACAGTTTCAACAGGAGTT ACAAATTACCACTGTTTTTGTCACCCATGACCAGGAAGAAGCGATGAGTA TTAGTGATGTCATTGTTTGTATGTCAACTGGAAAAGTGCAGCAAATCGGC ACACCCAGTGAACTTTATTTAAAACCTGCTAATGAGTTTGTTGCGCGCTT TTTAGGCACCCCTGAGATGAACATCATTGAATGTAGTGTCAAAAACAACC AGTTGTTTTGAAACAACCATCTGTTAGTTACTGAGAGTTTTAAGCTTAAT GTAGAGAAACTCTTAGTTGGGTTTAGGTATGAACAACTAGTGGTCACTAC TAACAAAAGTAGTTTGCAAGCTAAACTAATTAACATTGAAAACTTAGGTA AACACTTAGTTGCTACCATTAGTTTGTTTGATACCACCTTATCAATGCGC TTAGAATTGAATAGCCACTTAAAAGTAGGTGATAGTTTAAATTTCATTAT TAAAGCTAACAACCTCCATTTTTTTGATATTGATACAAAACAACGGATTG AGATTTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1300 1350 1400 1450 1500 1550 1600 1650 1700 1750 1758
Transcription unit	TU_116
Empirical formula (pH 7.5)	H₁₈₈₃₉C₁₇₀₈₀N₇₂₉₀O₁₂₁₄₃P₁₇₅₈
Molecular weight (pH 7.5; Da)	574972.08

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.369079 (dimensionless) (Show evidence) Value: 0.369079 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	2.7 (min) (Show evidence) Value: 2.7 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	18163700
pI	3.75

Comments
Comments	sugar transport [PUB_0110, PUB_0439, PUB_0440]; sulfate/molybdate transport [PUB_0110];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Chen J, Anderson JB, DeWeese-Scott C, Fedorova ND, Geer LY, He S, Hurwitz DI, Jackson JD, Jacobs AR, Lanczycki CJ, Liebert CA, Liu C, Madej T, Marchler-Bauer A, Marchler GH, Mazumder R, Nikolskaya AN, Rao BS, Panchenko AR, Shoemaker BA, Simonyan V, Song JS, Thiessen PA, Vasudevan S, Wang Y, Yamashita RA, Yin JJ, Bryant SH. MMDB: Entrez's 3D-structure database. Nucleic Acids Res 31, 474-7 (2003). WholeCell: PUB_0110, PubMed: 12520055, URL: http://www.ncbi.nlm.nih.gov/Structure/ Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Ren Q, Chen K, Paulsen IT. TransportDB: a comprehensive database resource for cytoplasmic membrane transport systems and outer membrane channels. Nucleic Acids Res 35, D274-9 (2007). WholeCell: PUB_0440, PubMed: 17135193, URL: http://membranetransport.org/ Saier MH Jr, Tran CV, Barabote RD. TCDB: the Transporter Classification Database for membrane transport protein analyses and information. Nucleic Acids Res 34, D181-6 (2006). WholeCell: PUB_0439, PubMed: 16381841, URL: http://www.tcdb.org/ Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:18
Last updated	2012-10-01 15:13:06