MG_183 – oligoendopeptidase F

Name
WID	MG_183
Name	oligoendopeptidase F
Symbol	pepF
Protein product	MG_183_MONOMER
Cross references	GenBank: AAC71402.1, CMR: MG_183, BioCyc: MG_183, SwissProt: P47429
Homologs	B. subtilis: BSU11540 M. hyopneumoniae: mhp520 M. mobile: mhp520 M. pneumoniae: MPN_197

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 208567 (nt), Length: 1824 (nt), Direction: Forward, G/C content: 32.2%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151 1201 1251 1301 1351 1401 1451 1501 1551 1601 1651 1701 1751 1801 ATGAAAAATAGTTACAAGTGGGATCTATCAGTTTTATTAAACAACCAAAG CTTACAAGCAAATTTTTTAAAAATTCAAACAGTTAGTGAAGCGTTAATTA AAGCTTATAACAACGGGTTGTGTTTTACAAATAAAACTAGCTTTGAACAG TTTTTAGCAATCGATGATAAGTTCACTGAACTTGAAAATCGTTACACTAA CTACCTTTATAACAAGCAGAATGAAAATAACTTGGATAAGGAGGTTAATG ATGCAATTTTTGCATACCAGAGTTTTAAAAATAACCATAACCTTGCTTTC AGTACACTGCAACAGGAGTTATATAACCATGAAAAACTCATTAAGGATTA TTTAACTGATCCAAAGCTAGCGGTTTACAAGCGCAACTTAATGTTAGTTT TTCGCGATAAACCCCACCAACTATCTAGTCAAACCCAGAGTTTATTGAGT CAAATTAACCCTTGTTTTAACCAAGCAGAACGGATTTTTAACATCCTTTC AACTGCTGATTTAAACTTGCAACCTGTTGTTTATCAAAACAAAAAATATC CGATTAACAGTGTGAGTGATTATCAGTCCTTACTTGAAAACACTAACAGG GGGATTAGAAAAGCTTGTTATGAGAAGTGGATTGAAATTTATTGAACTAA TAGAAACAGCTTAAGTTTAAGTTTGGTGGAAAATTACATCCAACTAGAGA ACTTCGCTAAACTAAAGAACCATCCTAGTTACATTGCCCAAACTGCTTTT AATGATGAGATTGAAGTTGGTTTTATTGATTTTGTTTACCAACAAGTAGC TCAGTTTGCCAAAACCTTTCAAGCATTTATTCGCTTAAAAAAACAGATTT ATAAACATGTTTTAAAAGTCAATAAAGTTGAACCATATGATCTTACCCTA ACACTTTTTAAAACTAAAAAATCATACACGATTGAACAAGCTAAACAAGA TGCACTAAAAGTTTTAGATCTACTTGGTGACAACTACATCAAGATAGTTA AAAAGGCTTTTAATGAAAACTGGATTGATTGACTAGCAGATAAAAACAAG TACACAGGGGCATATTCAATCTCCAATGTTAAGGGCTTAGAGCACTTTTT TATCTTAATGAACTTTGATAAAACCAAATCATCACTAAATACGTTGGTAC ATGAACTTGGTCATTCAGTTCACTCTTGGTATGCATCACAACACCAATCC CAAAACATCGATCCTACTATCTTTTATGCTGAGATTGCTTCTATTGCCAA TGAACTGTTGTTGTGTTATTATGAGCTGCAACTTTATAAAAATAACCACA AGCAGTTAATTGCTAGTTTATTGAGTCAAATCAACCATTTTTTTGGCGCT ACTACAAGACAAATAATGTTTTCACAATTTGAAAAAGATACGCTTTATTT AATCAGAGTTAACCAGAAACCTGACTTTAAAACTTTGATTAAAATTTATG CAAATACTGCGGTTAAATACCAAGGTTTTAAACCTGAAGTAGTTGCTAAT AAACTAAAAAAGACCCAGTATCAAAAATCATTGTCACACATCATTGCTAT CCCCCATTTTTATGCAGGTAACTTCTATGTTTACAAGTATGCCATTGGCC AAGTTGCAGGTATTTTAGTAGCTAAAAAAATTAATAGTGGTGATAAAAAG ATGAAAGATAATTACTTTAAATTCCTCAGTTCAGGTTCTAGTTTAGCGCC ACTTGAAACCATTAAACTCTTAGGGATTGACCTTACTTCACCCCAACCCT GACAAGAAGCACATAACGAAGTAAAGCGTTGGCTTAAAATTGTGAAACAA AGCTTTAAAAAACTCCAAAAATAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1300 1350 1400 1450 1500 1550 1600 1650 1700 1750 1800 1824
Transcription unit	TU_113
Empirical formula (pH 7.5)	H₁₉₅₀₃C₁₇₆₇₈N₇₄₃₄O₁₂₆₅₆P₁₈₂₄
Molecular weight (pH 7.5; Da)	595092.65

Functional genomics
Is essential	No (Show evidence) Value: False Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.560583 (dimensionless) (Show evidence) Value: 0.560583 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	18802100
pI	3.85

Comments
Comments	cleaves peptides 5-21 aa long [PUB_0035]; cleaves after hydrophobic and basic residues [PUB_0035]; broad specificity, never cleaves bonds involving acidic residues [PUB_0036]
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Chao SH, Cheng TH, Shaw CY, Lee MH, Hsu YH, Tsai YC. Characterization of a novel PepF-like oligopeptidase secreted by Bacillus amyloliquefaciens 23-7A. Appl Environ Microbiol 72, 968-71 (2006). WholeCell: PUB_0035, PubMed: 16391147 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Monnet V, Nardi M, Chopin A, Chopin MC, Gripon JC. Biochemical and genetic characterization of PepF, an oligopeptidase from Lactococcus lactis. J Biol Chem 269, 32070-6 (1994). WholeCell: PUB_0036, PubMed: 7798200 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:18
Last updated	2012-10-01 15:13:05