MG_146 – UPF0053 protein

Name
WID	MG_146
Name	UPF0053 protein
Protein product	MG_146_MONOMER
Cross references	GenBank: AAC71364.1, CMR: MG_146, BioCyc: MG_146, SwissProt: Q49399
Homologs	B. subtilis: BSU31300 M. pneumoniae: MPN_159 S. oneidensis: SO_1178

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 184875 (nt), Length: 1275 (nt), Direction: Forward, G/C content: 31.7%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151 1201 1251 ATGGACAGTGCCCCCAGTGGTTTAACTTTAACTGTTATTATCCTTAGCAT CATTCTGCTTGCTTTTATCAGCACAGTTGTATCAGCTTATGAAACAGCAA TCACTTCTTTAACCCCTTACAGGTGAAAGAACTATATCAAGACTAACAAC AAGCAAGATAAACTATCAACTAAGATAATAAACCACTTTCAAAACCACTA TTCAAGTTGTTTAATTACTATCCTAATTACTAACAACATAGTGGCCATTA TGGTTTCTAACATCCTTTTTTTAGCACTAGAACAAACAATTAAAAATGAG CTTTTATCAAGTGTTTTAAATTTGGTAGTTAGTGGGGTTTTAATCGTCTC TTTTTGTGAAATTCTACCCAAAACTTTGGGCAGAATTAATGTGATTAGAA CCCTGGTTCTATTTGCTTATTTGGTTTATTTTTTTTATCTGATCTTTTGA CCAATTACTAAGCTAACCAGTTTAATTCTCAAAAAGTATGAAAACCCCTT ACCTGTTTCAAGGAAAGATGTTTATTATTTTATTGATGAAATTGAACAAA ACGGTTTATTTTCCAAAGAAGATAGTTTACTGATTAAAAAAACCTTAATC TTTGACCAAGTACTAGTTAAAAAGGTAATGATCAAGTGAAAAAAAGTGGC TTATTGTTATCTTAATGACAGTATTAACTTGATTGCCAAGCAGTTTTTAC AAAGGCAGTTTTCCAGAATGCCAGTAGTAGATAAAACTACTAATAAGATA GTTGGTTTTATCCATTTAAAGGATTTTTTTACAGCTAAAGAAGCAAACCC TAAGTCACTTGATTTAAACCAGTTGCTTTATCCAGTTGTTTTAGTTCAAG ATTCCACCCCCATCAAACAAGCACTAAGACAGATGCGTTTAAACAGAGCA CATTTAGCAGTTGTTAATGATAAACATGAAAAAACAATAGGGATTGTTTC TATGGAGGATATTATTGAAGAGTTGGTGGGTGAAATCTATGATGAACATG ATGATATCCAACCGATCCAAGTATTGGATGAAAATGTTTGACTTGTTTTA CCTAATGTAAAAGCAGCCTACTTTTTTAATAAGTGAATTAAGCCAGATTT GGTAAAATCAAAAAATATTACTATCCAGCATTATCTCGCTTCACTAGATA ATGATAGTTTTGCTTGCCAAAATAAGCTTGACACTCCCTTATTTAGTGTT GAAGTGATAGCTGATAGCGAAGATAAAACCAAAATTCTTTACGAAATTAG AAAGAAGAGTGATGTTATTGCTTAG 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1275
Transcription unit	TU_094
Empirical formula (pH 7.5)	H₁₃₅₉₅C₁₂₃₁₉N₅₀₈₂O₈₉₁₇P₁₂₇₅
Molecular weight (pH 7.5; Da)	415002.51

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.851454 (dimensionless) (Show evidence) Value: 0.851454 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	13137700
pI	4.07

Comments
Comments	Hemolysins and related proteins containing CBS domains [PUB_0110]; hemolytic activity observed Mycoplasma penetrans [PUB_0185];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Chen J, Anderson JB, DeWeese-Scott C, Fedorova ND, Geer LY, He S, Hurwitz DI, Jackson JD, Jacobs AR, Lanczycki CJ, Liebert CA, Liu C, Madej T, Marchler-Bauer A, Marchler GH, Mazumder R, Nikolskaya AN, Rao BS, Panchenko AR, Shoemaker BA, Simonyan V, Song JS, Thiessen PA, Vasudevan S, Wang Y, Yamashita RA, Yin JJ, Bryant SH. MMDB: Entrez's 3D-structure database. Nucleic Acids Res 31, 474-7 (2003). WholeCell: PUB_0110, PubMed: 12520055, URL: http://www.ncbi.nlm.nih.gov/Structure/ Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Kannan TR, Baseman JB. Hemolytic and hemoxidative activities in Mycoplasma penetrans. Infect Immun 68, 6419-22 (2000). WholeCell: PUB_0185, PubMed: 11035754 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:16
Last updated	2012-10-01 15:12:59