MG_141 – transcription termination factor NusA

Name
WID	MG_141
Name	transcription termination factor NusA
Symbol	nusA
Protein product	MG_141_MONOMER
Cross references	GenBank: AAC71359.1, CMR: MG_141, BioCyc: MG_141, SwissProt: P47387
Homologs	E. coli: b3169 B. subtilis: BSU16600 M. hyopneumoniae: mhp603 M. mobile: mhp603 M. pneumoniae: MPN_154 S. oneidensis: SO_1203

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 179151 (nt), Length: 1596 (nt), Direction: Forward, G/C content: 32.5%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151 1201 1251 1301 1351 1401 1451 1501 1551 ATGAAGATAACTTTCATTTCTGGACAAGAAGTGTCGTTAGGCACTTCTTT TTTATTGTTTTCAAAAAAAATAGTTATGAATGAATTAAACCAACCCTTAC TTGCTATTATTAAAAATGTTGCTAAAACCAAAAACCTTTCTATAGAAGAG GTGGTTTTTTGTTTGAAAACAGCTTTAGAACAAGCCTATAAAAAACACCT TAACTTTGTTAATGTTGAAGTTAACATTAACTTTGATAAGGGGATTATTA ATGTTGAACAACTCTTTAATGTTGTTAGTGATGAAAATGAAGATTATGAT GACTTTCTTGAAATCCCTTTACAAGCAGCTAACAAAATAAACAGTTCATT GCAATTAGGTGATGTGTTGCGAAAACCAATCCCCTTAAAAAACATTAGTA GTGATCTTATCAATAAGATGATTGCTATCTTTAACCAAAAGATTAGTGAA ACAAACTTTAAAGCAGTAATGAGTGAGTTTAGTAGTGAGGTTGGGGAAGT GATTGAAGCGAAAGTTGAAGATATTGATACTAACAAAGAAGGTGGTTTAA AGGGTTATATTATTAACCTTGAAACTACAAAGGGTTATATCTCCAAGCGG GAATTGTCAAAAGGGGAGCGCTTAGAGATAGGTAAAAAATACCTCTTTGT TATCAAAGAAATCCAACGGCAAGCATCGTTATGACCAATTACTTTATCAA GAAGTGATACCCGCTTACTACAGTTTTTGTTAACTTCAAATACTCCAGAA ATTGAAAATGGTACGATTGTAATCAAAAAGATTGAACGTTCCCCAGGAGT GAAATCAAAGATAGCAGTTATCTCCAATGATCCTGCAGTTGACCCAGTTG CTGCTATCTTAGGACCTAAGGGTGAGAAGATTAGGGGGATTAGTGAGGAA TTTAATGGTGAGATTATTGACATTGTCTTTTGGAATGAAGACAAGTTAAA GTTCTTAATTAATGCCATTTTACCTGCAGAAGTCATTGGTTATAACATCT TGCAGGATGATGAGCGTGATACTAGTATTGAAGTTGTTGTACCTGCAAAC CAAATTGCTAATGTTTTTGGTTTTAAAGGTGTAAACATTAGGTTAATTAG TAATTTAACAGGTTGAAATAGTGTTGATGTTTACAGTGAAAAAGATGCAA GTGAAGCCAACATTAAATTCACGAGGTTAAGCTTTGAACCTGAAGGGTTG TTTGGCATCAAAAAAAGAAGGGAAAAGATCATTAGTAATGATGCTACTGA TAAAGTCTTTTACACCTCTAAAGACAATGTGATAGATGATGAGATTATTG TTGATTTAGCTAAAGATCTAATGGTTGATAATAAACAAAAACAACCTGAG CAAGTTGCAAAGCAAGTTGTTGAAAAATCACAATTAGAAAAACAAGTTAC TCCAAAAGAAAAAGAGAAAGTTCAACCAAAAGCTAAGGTTCATTCTAATA GCCATTCCAAAAAACCAGCTAAACCTAATCAGATTTTTTCTATCACTGTT GATGCTAGTGATAAGAATCTTAAAAAAGATCAAGTTGATAATAACCAAAC AAACCCCCAAACAAAACAAACATTTGATAGCTTTGATGATCTTTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1300 1350 1400 1450 1500 1550 1596
Transcription unit	TU_090
Empirical formula (pH 7.5)	H₁₇₀₇₄C₁₅₄₇₇N₆₅₃₁O₁₁₀₆₂P₁₅₉₆
Molecular weight (pH 7.5; Da)	520995.79

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.369079 (dimensionless) (Show evidence) Value: 0.369079 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	3.6 (min) (Show evidence) Value: 3.6 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	16648700
pI	3.87

Comments
Comments	transcription antitermination has not been reported in any mycoplasma [PUB_0182]; rho not essential in B. subtilis [PUB_0234]; the five sequenced members of the pneumoniae clade all contain most of the necessary machinery for forming an [PUB_0182]; binds RNA polymerase and promotes termination at certain RNA hairpin structures [PUB_0110]; binds Rho and RNA polymerase [PUB_0233]; helps bind Rho to elongation complex [PUB_0233]; slows RNA polymerase by enhancing pausing [PUB_0233];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Chen J, Anderson JB, DeWeese-Scott C, Fedorova ND, Geer LY, He S, Hurwitz DI, Jackson JD, Jacobs AR, Lanczycki CJ, Liebert CA, Liu C, Madej T, Marchler-Bauer A, Marchler GH, Mazumder R, Nikolskaya AN, Rao BS, Panchenko AR, Shoemaker BA, Simonyan V, Song JS, Thiessen PA, Vasudevan S, Wang Y, Yamashita RA, Yin JJ, Bryant SH. MMDB: Entrez's 3D-structure database. Nucleic Acids Res 31, 474-7 (2003). WholeCell: PUB_0110, PubMed: 12520055, URL: http://www.ncbi.nlm.nih.gov/Structure/ Ciampi MS. Rho-dependent terminators and transcription termination. Microbiology 152, 2515-28 (2006). WholeCell: PUB_0233, PubMed: 16946247 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Peterson JD, Umayam LA, Dickinson T, Hickey EK, White O. The Comprehensive Microbial Resource. Nucleic Acids Res 29, 123-5 (2001). WholeCell: PUB_0182, PubMed: 11125067, URL: http://cmr.jcvi.org/ ... 2 more Washio T, Sasayama J, Tomita M. Analysis of complete genomes suggests that many prokaryotes do not rely on hairpin formation in transcription termination. Nucleic Acids Res 26, 5456-63 (1998). WholeCell: PUB_0234, PubMed: 9826772 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:16
Last updated	2012-10-01 15:12:59