MG_139_DIMER – ribonuclease J

Name
WID	MG_139_DIMER
Name	ribonuclease J

Structure
Biosynthesis	[c]: (2) MG_139_MONOMER ⇒ MG_139_DIMER
No. subunits	2
Empirical formula (pH 7.5)	H₉₁₉₄C₅₇₆₈N₁₅₄₆O₁₆₇₆S₄₄
Molecular weight (pH 7.5; Da)	128424.56
Extinction coefficient (260 nm, 25C, pH 7.0)	-85410.00
Half life (OD (600 nm) = 0.3, M9 media, 36C; min)	1200.00

Synthesis
Formation process	Process_MacromolecularComplexation
Localization	c

Function
Enzyme	RNA cleavage (AMP) [c]: ApAp + H2O ⇒ (2) AMP + H RNA cleavage (CMP) [c]: CpCp + H2O ⇒ (2) CMP + H RNA cleavage (GMP) [c]: GpGp + H2O ⇒ (2) GMP + H RNA cleavage (UMP) [c]: H2O + UpUp ⇒ H + (2) UMP
Complex subunit	ribonuclease J [c]: (2) MG_139_MONOMER ⇒ MG_139_DIMER

Parameters
Parameters	Parameter_0133: enzymeSpecificRate_RNAseJ = 0.37 1/s

Comments
Comments	Composition [PUB_0165].
References	de la Sierra-Gallay IL, Zig L, Jamalli A, Putzer H. Structural insights into the dual activity of RNase J. Nat Struct Mol Biol 15, 206-12 (2008). WholeCell: PUB_0165, PubMed: 18204464

Metadata
Created	2012-10-01 15:07:38
Last updated	2012-10-01 15:14:07