MG_110 – ribosome small subunit-dependent GTPase A

Name
WID	MG_110
Name	ribosome small subunit-dependent GTPase A
Symbol	rsgA
Synonyms	yjeQ
Protein product	MG_110_MONOMER
Cross references	GenBank: AAC71328.1, CMR: MG_110, BioCyc: MG_110, SwissProt: P47356
Homologs	E. coli: b4161 B. subtilis: BSU15780 M. pneumoniae: MPN_249 S. coelicolor: SCO6149 S. oneidensis: SO_0591

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 137255 (nt), Length: 837 (nt), Direction: Forward, G/C content: 29.3%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 ATGCCAGATTCTAACTTTGGGATAGTGCTTCAGTCATTAGCAAAACAATG CAAAGTATTTTGGAATAACCAGATAATAACTGCTTTTCCTCAAAAGAAGT TACAGTGAAAAAATGATTTTAAGTTGATGGTTGGTGATAGGGTTCAACTT GAAGATGGTGCGATCACTAAAGTTTTAGCTAGAAAAAATGAACTTACAAG ACCAAGGGTTGCTAATGTTGATCAGATTGTTTTAATCCAATCACTAGTTC AACCTAAGATTAACTGAATTCAACTGTTGAAATTATTGGTATATTTCAAT GCTAAATTAATTGATGAAATACCTATTTTAATAACAAAAACAGACCTTGA TTTTGATCCAATGGAAAAACAAAAGTTAATCGATTTAAAACAGTTTAACT ATCAGTTATTTTTTGTTTCTAAAAATGAACCACTACCTTCAGAATTAATC GATATTTTTAGTAAAAAACTAAGTGTTTTTACAGGTCAATCTGGTGTTGG TAAATCTAGCTTAATTAATCGTTTAGATCCTTCTTTAAAACAAAAGATTC AAGCCTTATCAGTTAATAAATTTGGTAAGAATACCACTACTAAAACAACA CTTTTTTCATTTAGAGGCGGTTTTATTTGTGACACCCCTGGTTTTAATGT AATTTCTATTAAAAACCTCAAAATTTTAGCAGCCCAACACTTTGTTGGTT TTCAGAAAATGATTAGTAAGTGTCATTTTTCTAACTGTTATCATCAGTAT GAAAAAGATTGCTTTGTAACCACAAGTGTTATGAAAAACAGATATCCTTC GTGATTGTATGAGAAGTATAGAAAAATGATTAATTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 837
Transcription unit	TU_068
Empirical formula (pH 7.5)	H₈₉₁₉C₈₀₈₁N₃₃₁₈O₅₈₄₆P₈₃₇
Molecular weight (pH 7.5; Da)	271979.72

Functional genomics
Is essential	No (Show evidence) Value: False Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.369079 (dimensionless) (Show evidence) Value: 0.369079 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	2.9 (min) (Show evidence) Value: 2.9 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	8692200
pI	4.08

Comments
Comments	3' maturation 16S rRNA [PUB_0101]; may play role in joining 30S and 50S subunits [PUB_0104]; depletion results in slow growth, accumulation 17S RNA [PUB_0101]; promotes dissociation of tRNA from ribosome [PUB_0102]; GTP hydrolysis promotes dissociation with ribosome [PUB_0102]; interacts with ribosome near A- and P-sites of 30S ribosome [PUB_0104]. Kinetic rate in H. sapiens 0.0005 1/s [PUB_0689].
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Bourne HR, Sanders DA, McCormick F. The GTPase superfamily: conserved structure and molecular mechanism. Nature 349, 117-27 (1991). WholeCell: PUB_0689, PubMed: 1898771 Britton RA. Role of GTPases in bacterial ribosome assembly. Annu Rev Microbiol 63, 155-76 (2009). WholeCell: PUB_0104, PubMed: 19575570 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Hase Y, Yokoyama S, Muto A, Himeno H. Removal of a ribosome small subunit-dependent GTPase confers salt resistance on Escherichia coli cells. RNA 15, 1766-74 (2009). WholeCell: PUB_0101, PubMed: 19620234 ... 2 more Kimura T, Takagi K, Hirata Y, Hase Y, Muto A, Himeno H. Ribosome-small-subunit-dependent GTPase interacts with tRNA-binding sites on the ribosome. J Mol Biol 381, 467-77 (2008). WholeCell: PUB_0102, PubMed: 18588897 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:14
Last updated	2012-10-01 15:12:54