MG_097 – uracil-DNA glycosylase, putative

Name
WID	MG_097
Name	uracil-DNA glycosylase, putative
Symbol	ung
Protein product	MG_097_MONOMER
Cross references	GenBank: AAC71315.1, CMR: MG_097, BioCyc: MG_097, SwissProt: P47343
Homologs	E. coli: b2580 B. subtilis: BSU37970 M. hyopneumoniae: mhp251 M. mobile: mhp251 M. pneumoniae: MPN_235 ... 2 more S. coelicolor: SCO1343 S. oneidensis: SO_3654

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 123580 (nt), Length: 738 (nt), Direction: Forward, G/C content: 32.2%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 GTGGTAATGGATGATCTATTCCAAAGAATGGTTAGCTGTGTTCTACCGTC ATGAAGAGCTTTTATTGATGAGGAAGTTAAAAAACCTTATTTTCAAGCTT TATTAGAAAAATTAAAGGCTTTAAAAGCAACAATAATTCCAAAACCAGAA CTTATTTTCCGTGTTTTTAGCTTCTTTAAGCCAATTGATACAAAGGTAAT TATCTTTGGTCAAGATCCCTATCCTAGTCCTAATGATGCTTGTGGACTTG CTTTTGCATCCAATAATTCCAAAACCCCTGCCAGCTTAAAAAGAATAATT TTACGTTTAGAAAAAGAATATCCTTCGCTTAAACAAGAAAGTAGTTGACA ACAAAACTTCCTATTGAATTGAGCAGAACAGGGCGTTTTATTACTAAATG GAATTTTAACAACTACTGTATTTATACGCAACGCCCATAAAAATTGGGGT TGGGAGGAGTTTAACTGTAATTTGCTAACTTTTCTAAAAAATCAAAACAT TAAACCGCTGTTGGTATTTCTGGGTGTTCAAACTAAAAACTTTGTTGTTA AGAGTATTGGTAATGTTGATGGATTTGAGCATTTATCATATCCCCATCCC TCACCACTAAGTGGTAATTTGTTTCTAACAAACCCTAACGATCTGTTTAA AACAATTAACAATTGGTTGAAACAACATAACCAAAAAATAATTAACTGAG CAGTTGTTAAAAATGCTAGTTTTGACCAATTAAGTTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 738
Transcription unit	TU_061
Empirical formula (pH 7.5)	H₇₈₇₇C₇₁₃₈N₂₉₆₄O₅₁₅₁P₇₃₈
Molecular weight (pH 7.5; Da)	240459.04

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.851454 (dimensionless) (Show evidence) Value: 0.851454 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	5.2 (min) (Show evidence) Value: 5.2 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	7569800
pI	4.01

Comments
Comments	Excises uracil bases (2'-deoxyuridine, 5'-hydroxy-2'-deoxyuridine) from DNA generated through deamination of cytosine [PUB_0072, PUB_0461].
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Carvalho FM, Fonseca MM, Batistuzzo De Medeiros S, Scortecci KC, Blaha CA, Agnez-Lima LF. DNA repair in reduced genome: the Mycoplasma model. Gene 360, 111-9 (2005). WholeCell: PUB_0072, PubMed: 16153783 Friedberg EC, Walker GC, Siede W, Wood RD, Schultz RA, Ellenberger T. DNA repair and mutagenesis. ASM Press (2006). WholeCell: PUB_0461, ISBN: 9781555813192 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:14
Last updated	2012-10-01 15:12:52