MG_094_HEXAMER – replicative DNA helicase

Name
WID	MG_094_HEXAMER
Name	replicative DNA helicase
Cross references	PDB: 1JWE

Structure
Biosynthesis	[c]: (6) MG_094_MONOMER ⇒ MG_094_HEXAMER
No. subunits	6
DNA footprint	Length: 20 (nt), Binding: ssDNA, Region: xsDNA
Empirical formula (pH 7.5)	H₂₃₀₂₈C₁₄₄₅₄N₃₉₄₂O₄₃₆₂S₄₈
Molecular weight (pH 7.5; Da)	323355.49
Extinction coefficient (260 nm, 25C, pH 7.0)	-140280.00
Half life (OD (600 nm) = 0.3, M9 media, 36C; min)	1200.00

Synthesis
Formation process	Process_MacromolecularComplexation
Localization	c

Function
Enzyme	DNA unwinding [c]: ATP + H2O ⇒ ADP + H + PI
Reaction participant	DNA bound protein release (replicative DNA helicase releases heat-inducible transcription repressor HrcA, putative) MG_094_HEXAMER[c] + MG_205_DIMER[d] ⇒ MG_094_HEXAMER[d] + MG_205_DIMER[c] DNA bound protein release (replicative DNA helicase releases DnaA-ADP) MG_094_HEXAMER[c] + MG_469_1MER_ADP[d] ⇒ MG_094_HEXAMER[d] + MG_469_1MER_ADP[c] DNA bound protein release (replicative DNA helicase releases DnaA 7mer-(6)ATP-(1)ADP) MG_094_HEXAMER[c] + MG_469_7MER_6ATP_ADP[d] ⇒ MG_094_HEXAMER[d] + MG_469_7MER_6ATP_ADP[c] DNA bound protein release (replicative DNA helicase releases adenine-specific DNA modification methylase) MG_094_HEXAMER[c] + MG_184_DIMER[d] ⇒ MG_094_HEXAMER[d] + MG_184_DIMER[c] DNA bound protein release (replicative DNA helicase releases DNA-directed RNA polymerase) MG_094_HEXAMER[c] + RNA_POLYMERASE[d] ⇒ MG_094_HEXAMER[d] + RNA_POLYMERASE[c] ... 34 more DNA bound protein release (replicative DNA helicase releases ferric uptake repressor) MG_094_HEXAMER[c] + MG_236_MONOMER[d] ⇒ MG_094_HEXAMER[d] + MG_236_MONOMER[c] DNA bound protein release (replicative DNA helicase releases DnaA 2mer-(1)ATP-(1)ADP) MG_094_HEXAMER[c] + MG_469_2MER_1ATP_ADP[d] ⇒ MG_094_HEXAMER[d] + MG_469_2MER_1ATP_ADP[c] DNA bound protein release (DNA incision complex releases replicative DNA helicase) MG_073_206_421_TETRAMER[c] + MG_094_HEXAMER[d] ⇒ MG_073_206_421_TETRAMER[d] + MG_094_HEXAMER[c] DNA bound protein release (apurinic endonuclease releases replicative DNA helicase) MG_094_HEXAMER[d] + MG_235_MONOMER[c] ⇒ MG_094_HEXAMER[c] + MG_235_MONOMER[d] DNA bound protein release (replicative DNA helicase releases DnaA 6mer-(5)ATP-(1)ADP) MG_094_HEXAMER[c] + MG_469_6MER_5ATP_ADP[d] ⇒ MG_094_HEXAMER[d] + MG_469_6MER_5ATP_ADP[c] DNA bound protein release (replicative DNA helicase releases DNA ligase, NAD-dependent) MG_094_HEXAMER[c] + MG_254_MONOMER[d] ⇒ MG_094_HEXAMER[d] + MG_254_MONOMER[c] DNA bound protein release (replicative DNA helicase releases DNA topoisomerase I) MG_094_HEXAMER[c] + MG_122_MONOMER[d] ⇒ MG_094_HEXAMER[d] + MG_122_MONOMER[c] DNA bound protein release (Holliday junction endonuclease releases replicative DNA helicase) MG_094_HEXAMER[d] + MG_352_DIMER[c] ⇒ MG_094_HEXAMER[c] + MG_352_DIMER[d] DNA bound protein release (replicative DNA helicase releases DnaA-ATP 7mer) MG_094_HEXAMER[c] + MG_469_7MER_ATP[d] ⇒ MG_094_HEXAMER[d] + MG_469_7MER_ATP[c] DNA bound protein release (recombination protein, strand exchange releases replicative DNA helicase) MG_094_HEXAMER[d] + MG_339_MONOMER[c] ⇒ MG_094_HEXAMER[c] + MG_339_MONOMER[d] DNA bound protein release (formamidopyrimidine-DNA glycosylase releases replicative DNA helicase) MG_094_HEXAMER[d] + MG_498_MONOMER[c] ⇒ MG_094_HEXAMER[c] + MG_498_MONOMER[d] DNA bound protein release (replicative DNA helicase releases DnaA-ATP 2mer) MG_094_HEXAMER[c] + MG_469_2MER_ATP[d] ⇒ MG_094_HEXAMER[d] + MG_469_2MER_ATP[c] DNA bound protein release (replicative DNA helicase releases Spx subfamily protein) MG_094_HEXAMER[c] + MG_127_MONOMER[d] ⇒ MG_094_HEXAMER[d] + MG_127_MONOMER[c] DNA bound protein release (replicative DNA helicase releases type I restriction modification DNA specificity domain protein) MG_094_HEXAMER[c] + MG_438_MONOMER[d] ⇒ MG_094_HEXAMER[d] + MG_438_MONOMER[c] DNA bound protein release (replicative DNA helicase releases single-strand binding protein family, octamer) MG_091_OCTAMER[d] + MG_094_HEXAMER[c] ⇒ MG_091_OCTAMER[c] + MG_094_HEXAMER[d] DNA bound protein release (replicative DNA helicase releases DnaA-ATP 3mer) MG_094_HEXAMER[c] + MG_469_3MER_ATP[d] ⇒ MG_094_HEXAMER[d] + MG_469_3MER_ATP[c] DNA bound protein release (replicative DNA helicase releases LuxR bacterial regulatory protein, putative) MG_094_HEXAMER[c] + MG_428_DIMER[d] ⇒ MG_094_HEXAMER[d] + MG_428_DIMER[c] DNA bound protein release (Holliday junction DNA helicase releases replicative DNA helicase) MG_094_HEXAMER[d] + MG_358_359_10MER[c] ⇒ MG_094_HEXAMER[c] + MG_358_359_10MER[d] DNA bound protein release (replicative DNA helicase releases DnaA-ATP 6mer) MG_094_HEXAMER[c] + MG_469_6MER_ATP[d] ⇒ MG_094_HEXAMER[d] + MG_469_6MER_ATP[c] DNA bound protein release (replicative DNA helicase releases DnaA 4mer-(3)ATP-(1)ADP) MG_094_HEXAMER[c] + MG_469_4MER_3ATP_ADP[d] ⇒ MG_094_HEXAMER[d] + MG_469_4MER_3ATP_ADP[c] DNA bound protein release (replicative DNA helicase releases DnaA 5mer-(4)ATP-(1)ADP) MG_094_HEXAMER[c] + MG_469_5MER_4ATP_ADP[d] ⇒ MG_094_HEXAMER[d] + MG_469_5MER_4ATP_ADP[c] DNA bound protein release (replicative DNA helicase releases Chromosome Segregation Protein SMC with SCP Proteins-ADP) MG_094_HEXAMER[c] + MG_213_214_298_6MER_ADP[d] ⇒ MG_094_HEXAMER[d] + MG_213_214_298_6MER_ADP[c] DNA bound protein release (replicative DNA helicase releases DnaA-ATP) MG_094_HEXAMER[c] + MG_469_1MER_ATP[d] ⇒ MG_094_HEXAMER[d] + MG_469_1MER_ATP[c] DNA bound protein release (replicative DNA helicase releases DnaA-ATP 4mer) MG_094_HEXAMER[c] + MG_469_4MER_ATP[d] ⇒ MG_094_HEXAMER[d] + MG_469_4MER_ATP[c] DNA bound protein release (replicative DNA helicase releases DNA-directed RNA polymerase holoenzyme) MG_094_HEXAMER[c] + RNA_POLYMERASE_HOLOENZYME[d] ⇒ MG_094_HEXAMER[d] + RNA_POLYMERASE_HOLOENZYME[c] DNA bound protein release (polI-like 5'-3' exonuclease, putative releases replicative DNA helicase) MG_094_HEXAMER[d] + MG_262_MONOMER[c] ⇒ MG_094_HEXAMER[c] + MG_262_MONOMER[d] DNA bound protein release (replicative DNA helicase releases Uncharacterized HTH-type transcriptional regulator) MG_094_HEXAMER[c] + MG_101_MONOMER[d] ⇒ MG_094_HEXAMER[d] + MG_101_MONOMER[c] DNA bound protein release (replicative DNA helicase releases topoisomerase IV) MG_094_HEXAMER[c] + MG_203_204_TETRAMER[d] ⇒ MG_094_HEXAMER[d] + MG_203_204_TETRAMER[c] DNA bound protein release (replicative DNA helicase releases DNA gyrase) DNA_GYRASE[d] + MG_094_HEXAMER[c] ⇒ DNA_GYRASE[c] + MG_094_HEXAMER[d] DNA bound protein release (uracil-DNA glycosylase, putative releases replicative DNA helicase) MG_094_HEXAMER[d] + MG_097_MONOMER[c] ⇒ MG_094_HEXAMER[c] + MG_097_MONOMER[d] DNA bound protein release (replicative DNA helicase releases DnaA 3mer-(2)ATP-(1)ADP) MG_094_HEXAMER[c] + MG_469_3MER_2ATP_ADP[d] ⇒ MG_094_HEXAMER[d] + MG_469_3MER_2ATP_ADP[c] DNA bound protein release (replicative DNA helicase releases DnaA-ATP 5mer) MG_094_HEXAMER[c] + MG_469_5MER_ATP[d] ⇒ MG_094_HEXAMER[d] + MG_469_5MER_ATP[c] DNA bound protein release (DNA polymerase III, beta subunit releases replicative DNA helicase) MG_001_MONOMER[c] + MG_094_HEXAMER[d] ⇒ MG_001_MONOMER[d] + MG_094_HEXAMER[c] DNA bound protein release (3-5' helicase releases replicative DNA helicase) MG_094_HEXAMER[d] + MG_244_DIMER[c] ⇒ MG_094_HEXAMER[c] + MG_244_DIMER[d]
Complex subunit	replicative DNA helicase [c]: (6) MG_094_MONOMER ⇒ MG_094_HEXAMER Replisome [c]: (2) DNA_POLYMERASE_CORE + DNA_POLYMERASE_GAMMA_COMPLEX + (4) MG_001_MONOMER + MG_094_HEXAMER + MG_250_MONOMER ⇒ REPLISOME

Parameters
Parameters	Parameter_0145: rnaPolymeraseCollisionMeanDwellTime = 1.7 s

Comments
Comments	Composition [PUB_0201]. DNA-binding DnaB occipies 17-23 nt [PUB_0628].
References	Bujalowski W, Jezewska MJ. Interactions of Escherichia coli primary replicative helicase DnaB protein with single-stranded DNA. The nucleic acid does not wrap around the protein hexamer. Biochemistry 34, 8513-9 (1995). WholeCell: PUB_0628, PubMed: 7612593 Mitkova AV, Khopde SM, Biswas SB. Mechanism and stoichiometry of interaction of DnaG primase with DnaB helicase of Escherichia coli in RNA primer synthesis. J Biol Chem 278, 52253-61 (2003). WholeCell: PUB_0201, PubMed: 14557266

Metadata
Created	2012-10-01 15:07:37
Last updated	2012-10-01 15:14:05