MG_086 – prolipoprotein diacylglyceryl transferase

Name
WID	MG_086
Name	prolipoprotein diacylglyceryl transferase
Symbol	lgt
Synonyms	gerF
Protein product	MG_086_MONOMER
Cross references	GenBank: AAC71304.1, CMR: MG_086, BioCyc: MG_086, SwissProt: P47332
Homologs	B. subtilis: BSU34990 M. hyopneumoniae: mhp282 M. mobile: mhp282 M. pneumoniae: MPN_224

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 112718 (nt), Length: 1149 (nt), Direction: Forward, G/C content: 32.1%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 ATGAATAGACCAAGTTGATCAACTGCATTTAATATTGGTGGTGGATTTCC CATCCAGTGGTATGGGATCATTGTCTCAATTGGCATTATTTTTGCCATTT TAATGTTTGTCTTTAAACTGATTTATTGTTACAAATTACAAGACAACAGT TTTTATTTTTTTATCTTTATTGCTGTTTTAACGATGGTTTTAGGCGCTCG CCTCTGGTCATTTGTAATTGGTGATTCCAATTTTGCTAACAACAATTTCT TTGATTTTCGTAACGGTGGATTGGCCATTCAGGGTGGGATTTTGTTAACC AGTATTGTCGGAGTAATCTATTTCAACTTCTTTTTAAATAGTAAGACCAA TAAAACCAAAACGATTGCTGAATTACTGAATAATAAGAATGAAATAAAAG CTGTTTATGTTGAAAGAAATATCTCTGTTCTAGTGATGTTAGATCTGATT GCTCCTTGTGTTTTAATTGGTCAAGCAATTGGCAGATGGGGTAATTTTTT CAACCAAGAAGTTTATGGGTTTGCTTTAGCTGGAACAATGAATGATCCCC AAGCATTGGCTAATACCCAGTGGGGATTTTTAAAGATCTTAATGCCTAAG GTTTGGGATGGGATGTGGATTGATGGTCAGTTTCGCATTCCGCTCTTTTT AATTGAGTCATTTTTTAACACTATTTTCTTTGTGTTAATTTACTTTGTAA TGGATTTTATTAGGGGAGTTAAAAGTGGCACAATTGGTTTTAGTTATTTT CTTGCTACTGGAATCATTCGTTTAATCTTGGAAAACTTTAGAGACCAAAC CTTTTATTTTCAAACTTCAATAACCACTAGTATTTTGTTTATTGTCGTTG GTATTTTAGGAATTTTTTATTGCCAGTTTATCCATGTCAAATTAAGAAAT TACTTCTGAACTTATTTCTTTCTTTATGCCTTTTATAAAGTAGCTGCTTT TTTCACTACACTTTTTTTGAATAACAGAAAGCAAATGGCACAACAGAAGT TTGCTTTTTATGAAAAATCACTTCCCAATAAGAAGCGTTCTTTTTTTGAA ATGAAGTATTACAATGATGTAACAACACCCAAAATTTATCGTTTAACTGA TCAGGAAATGAAGTTATTTGATAAATTAGAGGCAGTTACAACCAGCTAG 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1149
Transcription unit	TU_055
Empirical formula (pH 7.5)	H₁₂₁₉₀C₁₁₀₄₀N₄₃₉₅O₈₁₆₄P₁₁₄₉
Molecular weight (pH 7.5; Da)	372651.87

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.369079 (dimensionless) (Show evidence) Value: 0.369079 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	11704400
pI	4.86

Comments
Comments	Transfers the N-acyl diglyceride group on what will become the N-terminal cysteine of membrane lipoproteins [PUB_0096]; prolipoprotein + L-1-phosphatidyl-glycerol diacylglycerol prolipoprotein + sn-glycerol 1-phosphate [PUB_0266];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Sankaran K, Wu HC. Lipid modification of bacterial prolipoprotein. Transfer of diacylglyceryl moiety from phosphatidylglycerol. J Biol Chem 269, 19701-6 (1994). WholeCell: PUB_0266, PubMed: 8051048 UniProt Consortium. The Universal Protein Resource (UniProt) 2009. Nucleic Acids Res 37, D169-74 (2009). WholeCell: PUB_0096, PubMed: 18836194, URL: http://www.uniprot.org/uniprot/ Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:13
Last updated	2012-10-01 15:12:50