MG_083_MONOMER – peptidyl-tRNA hydrolase

Name
WID	MG_083_MONOMER
Name	peptidyl-tRNA hydrolase
Cross references	PDB: 2PTH

Genetics
Gene	MG_083

Structure
Sequence	1 51 101 151 MPTYKLIVGLGNLGKKYEKTRHNAGFMVLDRLASLFHLNFDKTNKLGDYL FIKEKAAILAKPATFMNNSGLFVKWLQDHFQIPLANIMIVHDEIAFDLGV IRLKMQGSANNHNGIKSVIRHLDTEQFNRLRFGIKSQNTSNILHEQVMSE FQNSELTKLEVAITKSVELLKRYIEGEELQRLMEYYHHG* 50 100 150 190
Is N‑terminal methionine cleaved	No
Empirical formula (pH 7.5)	H₁₅₆₂C₉₈₈N₂₇₀O₂₇₅S₇
Molecular weight (pH 7.5; Da)	21847.01
Extinction coefficient (260 nm, 25C, pH 7.0)	14440
Instability index	36.53
Is stable	True
Aliphatic index	97.00
GRAVY (25C, pH 7.0)	-0.269
Half life (OD (600 nm) = 0.3, M9 media, 36C; min)	1200

Synthesis
Localization	c
Chaperones	MG_392_393_21MER

Function
Enzyme	Peptidyl-tRNA hydrolysis (ALA) [c]: AMP_ALA + H2O ⇒ ALA + AMP Peptidyl-tRNA hydrolysis (ARG) [c]: AMP_ARG + H2O ⇒ AMP + ARG + H Peptidyl-tRNA hydrolysis (ASN) [c]: AMP_ASN + H2O ⇒ AMP + ASN Peptidyl-tRNA hydrolysis (ASP) [c]: AMP_ASP + H2O ⇒ AMP + ASP Peptidyl-tRNA hydrolysis (CYS) [c]: AMP_CYS + H2O ⇒ AMP + CYS ... 16 more Peptidyl-tRNA hydrolysis (MET) [c]: AMP_FMET + H2O ⇒ AMP + FMET Peptidyl-tRNA hydrolysis (GLN) [c]: AMP_GLN + H2O ⇒ AMP + GLN Peptidyl-tRNA hydrolysis (GLU) [c]: AMP_GLU + H2O ⇒ AMP + GLU + H Peptidyl-tRNA hydrolysis (GLY) [c]: AMP_GLY + H2O ⇒ AMP + GLY Peptidyl-tRNA hydrolysis (HIS) [c]: AMP_HIS + H2O ⇒ AMP + HIS Peptidyl-tRNA hydrolysis (ILE) [c]: AMP_ILE + H2O ⇒ AMP + ILE Peptidyl-tRNA hydrolysis (LEU) [c]: AMP_LEU + H2O ⇒ AMP + LEU Peptidyl-tRNA hydrolysis (LYS) [c]: AMP_LYS + H2O ⇒ AMP + LYS Peptidyl-tRNA hydrolysis (MET) [c]: AMP_MET + H2O ⇒ AMP + MET Peptidyl-tRNA hydrolysis (PHE) [c]: AMP_PHE + H2O ⇒ AMP + PHE Peptidyl-tRNA hydrolysis (PRO) [c]: AMP_PRO + H2O ⇒ AMP + PRO Peptidyl-tRNA hydrolysis (SER) [c]: AMP_SER + H2O ⇒ AMP + SER Peptidyl-tRNA hydrolysis (THR) [c]: AMP_THR + H2O ⇒ AMP + THR Peptidyl-tRNA hydrolysis (TRP) [c]: AMP_TRP + H2O ⇒ AMP + TRP Peptidyl-tRNA hydrolysis (TYR) [c]: AMP_TYR + H2O ⇒ AMP + TYR Peptidyl-tRNA hydrolysis (VAL) [c]: AMP_VAL + H2O ⇒ AMP + VAL

Parameters
Parameters	Parameter_0127: peptidylTRNAHydrolaseSpecificRate = 0.7 1/s

Comments
Comments	Functional Macromolecule Functional as monomer [PUB_0006].
References	Keseler IM, Bonavides-Martínez C, Collado-Vides J, Gama-Castro S, Gunsalus RP, Johnson DA, Krummenacker M, Nolan LM, Paley S, Paulsen IT, Peralta-Gil M, Santos-Zavaleta A, Shearer AG, Karp PD. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res 37, D464-70 (2009). WholeCell: PUB_0006, PubMed: 18974181

Metadata
Created	2012-10-01 15:07:47
Last updated	2012-10-01 15:14:25