MG_073_MONOMER – excinuclease ABC, B subunit

Name
WID	MG_073_MONOMER
Name	excinuclease ABC, B subunit
Cross references	PDB: 1QOJ

Genetics
Gene	MG_073

Structure
Sequence	1 51 101 151 201 251 301 351 401 451 501 551 601 651 MKNQTKSNSLFQLSTNYIPTGDQPEAIKKLSEFKTKQQVLLGATGTGKTF TIANVIQNSQLPTVVIAHNKTLAGQLFNELKQLFPKNAVEYFISYFDFYQ PEAYLPSKGIYIEKSATVNEAIKRLRVSTLHSLSTRKDVIVVGSVASIYP TSSPSDFVKYCLWFVVGKDYDLKTIKDRLVSLNYVVNKQQLTPGKFRFQG DVLEVFPGYSDAFVIRISFFDTKVEQICQIDPLTNKILNQLFEIKIGPAD EYVVNQSDLDIAIKNIKQELQERVNYFNKQNLVERAQRLATITNHDLNDL KAWGFCSGVENYARHLELRMANSTPYSIFDYFKGDWLLVIDESHQTLPQL NGMYNTDLSRKQSLIDYGFRLPSALDNRPLSFAELQQKMQKVIYVSATPR DKEISLSQNNVIEQLVRPTYLVDPIIVVKPKDNQVEDLIEEIINQRQNNT RTFVTVLTIKMAENLTEYLKERKIKVAYIHKDIKALERLLLINDLRRGEY ECLVGINLLREGLDVPEVALVCIFDADIPGLPRDERSLIQIIGRAARNEH GRVVMYANHVTEQMQKAIDETKRRRTVQMEYNKLHNKTPKTVVKPLTFVQ PIKLKAKSNAEKNAALIKQLTKEMKKAAANQNYELAIEIRDSIFELEKEI GSKIKV* 50 100 150 200 250 300 350 400 450 500 550 600 650 657
Is N‑terminal methionine cleaved	Yes
Empirical formula (pH 7.5)	H₅₄₃₉C₃₃₈₈N₉₁₁O₉₉₀S₁₄
Molecular weight (pH 7.5; Da)	75222.64
Extinction coefficient (260 nm, 25C, pH 7.0)	55865
Instability index	36.94
Is stable	True
Aliphatic index	99.09
GRAVY (25C, pH 7.0)	-0.324
Half life (OD (600 nm) = 0.3, M9 media, 36C; min)	1200

Synthesis
Localization	c

Function
Complex subunit	DNA incision complex [c]: MG_073_MONOMER + MG_206_MONOMER + (2) MG_421_MONOMER ⇒ MG_073_206_421_TETRAMER

Comments
Comments	First observed residue by mass-spec 1 [PUB_0280]. Functional Macromolecule Functional as hetercomplex (see MG_073_206_421_TRIMER). N-terminal methionine cleavage Shewanella oneidensis MR-1 homolog undergoes N-terminal methionine cleavage [PUB_0280].
References	Gupta N, Tanner S, Jaitly N, Adkins JN, Lipton M, Edwards R, Romine M, Osterman A, Bafna V, Smith RD, Pevzner PA. Whole proteome analysis of post-translational modifications: applications of mass-spectrometry for proteogenomic annotation. Genome Res 17, 1362-77 (2007). WholeCell: PUB_0280, PubMed: 17690205

Metadata
Created	2012-10-01 15:07:46
Last updated	2012-10-01 15:14:25