MG_060 – glycosyl transferase

Name
WID	MG_060
Name	glycosyl transferase
Symbol	rfbV
Protein product	MG_060_MONOMER
Cross references	GenBank: AAC71277.1, CMR: MG_060, BioCyc: MG_060, SwissProt: P47306
Homologs	M. pneumoniae: MPN_075

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 67650 (nt), Length: 894 (nt), Direction: Forward, G/C content: 27.3%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 ATGAAATTATCTGTAATTATACCTACTTACAATTGTGCATCATTTATTGA AAAAGCAATTAATTCAATTGTTAAAAATAGACCTAATGATTTGGAAATAG AAGTTTTAATTATTGATGATGGATCAATTGACAATACTAACAAAGTTATT AAGAAAATTCAAGACCAAATTAATAATTTAACTTTGCAGTATTTTTACAA AAGTAATGGTAACTGGGGTAGTGTTATTAATTATGTTAGAAACAATAAAC TAGCAAAAGGGGAATGAGTAACAGTATTGGATAGTGATGACATTTTTTCA AAAAAAACAATTTCTATTTTTCAAAAATATGCCCAAAAACAAAGATATGA TGCGATTATTTTTGACTACTATAAATGCTGAAAAAAGTTTTTGTGAAAAA TTCCTACCTATGCAAGGTTTAGAAAAGAAATTAAAGGTGAATTGAAAAAA CAAACACCTTTTTGTATTCCCTTAGCTAAGTTTTTTAAAAATGAGGTTTT CTATCAACTTCCTAAACTAAGAGAAAATGTTGGTTTTCAAGACGCTATTT ATACGATGCATGCATTACAAATTGCAAATAATGTTTTCCATGTTTCTAAA GCTGGAGGATATTACTTTTTTAAAAGGGTAGGTAACTCTATGAGTATCCC TTGACACAGTTCTAGGTTTGATATTGAAGTACAAATCTGCAAGGATCTGA TTGAAAATAATGCGCAAGAGATCGCTTTAGTGCATTTACTTCGTTTAAAA TTTCGTAATTTAGTTGATGATAAAAAGATTAAATTTACAGTTAAAAGAGA CTTTTGTTTTAGTGGTTTTAGTTGGTATAGTAGGTTAATTTTATCTCTGA TGTATAACTTCTGATTGAAACGTTATTTCAACAGTTCTGAATAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 894
Transcription unit	TU_035
Empirical formula (pH 7.5)	H₉₅₁₈C₈₆₂₃N₃₅₁₉O₆₂₄₃P₈₉₄
Molecular weight (pH 7.5; Da)	290025.87

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.560583 (dimensionless) (Show evidence) Value: 0.560583 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	9381400
pI	4.13

Comments
Comments	synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein, for example (malQ, coli): (glucose)n + (glucose)m (glucose)n+m-1 + alpha-D-glucose [PUB_0110]; essential for O-antigen biosynthesis in V. cholerae [PUB_0237]; group 6 nrd2 glycosyltransferases: abequose transferase, takes part in the formation of the O-antigen repeat unit [PUB_0238];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Chen J, Anderson JB, DeWeese-Scott C, Fedorova ND, Geer LY, He S, Hurwitz DI, Jackson JD, Jacobs AR, Lanczycki CJ, Liebert CA, Liu C, Madej T, Marchler-Bauer A, Marchler GH, Mazumder R, Nikolskaya AN, Rao BS, Panchenko AR, Shoemaker BA, Simonyan V, Song JS, Thiessen PA, Vasudevan S, Wang Y, Yamashita RA, Yin JJ, Bryant SH. MMDB: Entrez's 3D-structure database. Nucleic Acids Res 31, 474-7 (2003). WholeCell: PUB_0110, PubMed: 12520055, URL: http://www.ncbi.nlm.nih.gov/Structure/ Fallarino A, Mavrangelos C, Stroeher UH, Manning PA. Identification of additional genes required for O-antigen biosynthesis in Vibrio cholerae O1. J Bacteriol 179, 2147-53 (1997). WholeCell: PUB_0237, PubMed: 9079898 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Kapitonov D, Yu RK. Conserved domains of glycosyltransferases. Glycobiology 9, 961-78 (1999). WholeCell: PUB_0238, PubMed: 10521532 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:12
Last updated	2012-10-01 15:12:46