MG_048 – signal recognition particle protein

Name
WID	MG_048
Name	signal recognition particle protein
Symbol	ffh
Protein product	MG_048_MONOMER
Cross references	GenBank: AAC71264.1, CMR: MG_048, BioCyc: MG_048, SwissProt: P47294
Homologs	E. coli: b2610 B. subtilis: BSU15980 M. hyopneumoniae: mhp060 M. mobile: mhp060 M. pneumoniae: MPN_061 ... 2 more S. coelicolor: SCO5586 S. oneidensis: SO_1356

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 56969 (nt), Length: 1341 (nt), Direction: Reverse, G/C content: 33.2%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151 1201 1251 1301 ATGTTCAAAGCAATGTTATCAAGCATCGTTATGCGCACGATGCAAAAAAA AATTAACGCTCAAACGATCACTGAGAAAGATGTAGAGTTAGTTCTAAAAG AGATTAGAATTGCATTGCTTGATGCTGATGTTAACCTGCTTGTTGTTAAA AATTTCATCAAAGCAATTAGAGATAAAACAGTAGGACAAACCATTGAACC TGGTCAAGATTTGCAAAAGTCTCTATTAAAAACAATCAAAACAGAACTAA TTAATATCTTAAGCCAACCCAACCAAGAACTAAATGAAAAAAGACCTTTA AAAATAATGATGGTTGGTTTACAAGGATCAGGTAAAACAACAACTTGTGG CAAACTAGCTTATTGACTTGAAAAGAAATACAAGCAAAAAACAATGTTAG TAGGCTTGGACATCTACAGACCCGCTGCCATTGAACAACTTGAAACGCTT TCACAACAAACTAACAGCGTATTTTTTGCACAAGGCACTCAACCAGTTGC TAAAACAACAAAAGCAGCACTCAGTGCTTTTAAAACTGCAAAATGTCAAA CAATCATTTGTGATACCGCTGGTAGATTACAAACAAATGAAACATTAATG GATGAATTGGTAAGTGTTAAAAATGAATTAAATCCTGATGAAATTATCAT GGTAGTAGATGGATTAAGCGGTCAGGAAATTATCAATGTTGCTCAAACGT TCCACAAACGTTTAAAACTAACTGGATTTATTATCAGTAAATTAGACAGT GATGCTAGAGCAGGAGCTGCACTTTCATTAGCTTCACTTTTACAAGTACC CATTAAATTAATTGGTGTTTCTGAAAAATTAGATGGATTGGAACAATTTC ATCCTGAAAGGATAGCCAATCGGATCTTAGGTTTGGGTGATGTAATGAGT TTAGTTGAAAAAGCTGAACAAGTTTTTGATAAAAAAGATTTAACTAAAAC CATCAGCAAGATGTTTTTGGGAAAAATGGATTTAGAAGATCTTTTGATCT ACATGCAACAAATGCACAAAATGGGAAGTGTCAGTTCACTGATAAAAATG TTGCCTGCTAACTTTTCTGTATCAGAAGAAAATGCTGAATTAATTGAAAA CAAAATTGAACTATGAAAGGTTTTAATTAACTCTATGACTAGAGAAGAAA GGAGACATCCCAAATTAATTAATCGTGATCCTAATAGAAAACAGCGCATC ATAAAAGGATCAGGGAGAAAAATGGATGAGTTAAACAAACTGATGAAGGA ATGAAATAAGATGCAACTAAAAGCAACAGAAATGGGTAAACTATTAAAAA CAGGTAGTAACCCGTTTGGTGGATTTGGACAATTCTTTTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1300 1341
Transcription unit	TU_028
Empirical formula (pH 7.5)	H₁₄₃₉₀C₁₃₀₄₈N₅₆₁₉O₉₂₁₆P₁₃₄₁
Molecular weight (pH 7.5; Da)	438909.28

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	1.96428 (dimensionless) (Show evidence) Value: 1.96428 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	2.8 (min) (Show evidence) Value: 2.8 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	13986000
pI	3.68

Comments
Comments	half-life free ffh protein 20 minutes [PUB_0399];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Jensen CG, Pedersen S. Concentrations of 4.5S RNA and Ffh protein in Escherichia coli: the stability of Ffh protein is dependent on the concentration of 4.5S RNA. J Bacteriol 176, 7148-54 (1994). WholeCell: PUB_0399, PubMed: 7525539 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:11
Last updated	2012-10-01 15:12:44