MG_040 – Monosaccharide (riboside/galactoside) ABC transporter, substrate binding protein

Name
WID	MG_040
Name	Monosaccharide (riboside/galactoside) ABC transporter, substrate binding protein
Protein product	MG_040_MONOMER
Cross references	GenBank: AAC71256.1, CMR: MG_040, BioCyc: MG_040, SwissProt: P47286
Homologs	M. pneumoniae: MPN_052

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 47580 (nt), Length: 1776 (nt), Direction: Forward, G/C content: 32.5%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151 1201 1251 1301 1351 1401 1451 1501 1551 1601 1651 1701 1751 ATGGGAAAGCTATTATTTGGTAAGTTAGTTTTTAAAAAGAGCTTGTTTTT ACTGAGTGGTATGAGCAGTTTAGCAGTTTTTTTAACTGCATGTGGGGCTA CCAAAATATTTGATTCCTCTGTACAGCTATTAGTGTCAGATAACTTTTCC ACACTTGCTGATAAATCATTTTCACAAATGTCCTATGAAGGAATCAGGAG CTTTTTTAAAAAAAGTAAGGGTGTTGATTTACCTGAAGCAGATAGTTCAC AATTACAAGAAGGCAATGGCTTGTGAAAACGTCCTGGTTTTACATTAAGT GATAGAATTGCTACTTTTAATAACATCAAAAATGATGGCTCTGATGTTAT TGTTGCAACAGGTTTTAACCAACAAGAATCACTTCAAGCAATTACTTCTG ATGACATTAGGTTTCAAAGTGATAAAGAAAGCTTAGCTAAAACAGGTTTT ATTTTTGTTGATGGAGCTATTGAAAAGGAGTTTAATAAAAGAAATGGTGT TCCTCAATTTAAGTCAACTCCTACCAATATCTCATCAGTTGCTTTTAGAA GTGATGATGGTTCTTTTTTAACTGGAGTTGCTACTGCAGTTTACTTAAAT CTTAACCAAGAATATTTCCTTGATAAAAGTGGTTGGTCAACTAATAGTAG TAATAATAACGAACTAACTGTAAGTGGTTTTGTAGGTATTGCTCTACCTT CAACGCTTTCTTTTCTAAATGGTTTTCGACTAGGTATTGCTTATTTTAAT GAAGTGATTTATAAACATTTAAGCGATGCACAAGATTCATCTGCACAAGT GACCACTTCTAAACAAACTGTTTTAAAACAACTTCAAGTTGCAAATGGTG AAAAAAGGATTAAAAAGATTAAATGGATTTCACCAAAACAAGGAAGTGAT GGAGAAACTATAAACATTCAAGATCACCAATCAGGTTCTTTTTCAGATAC TGAACCTAGAGCAATAACAATAGCTAATAATTTAATTGATAAAGGAGTTA ATGCTATCATTCCTATTGCTGGACCACAAACGAATTTAGTGGTTACTCAA ATTGCTAGAAGACAAGCCCATACTGCAGTTATTGGCGTTGATAGTGCACA GGAATTGCTAGATATTAATATTGATGCTCCAAATAAAGATAAGTTAAAAA TGGGGAATAAAAAGATTATTCCCTTCTCTTCTATTAAGGCTTTGGATGTT GCTGTTGAAAGTATCTTATCAACATTAGAAAAAGGTTCCAGCCAAAATGG TTATCAAGGCTTTGGATATAACAACATAGGTACAGTGAAAAACAACTCTG TTGGGGTTAGTGAAGCAGGTTATGAATTTTTAATAGATCCTGTTTTTTGA AAAAATACTAGTTCAATGCAAGCTATGTCTTTATCAGCAAGTCTAAAAGC TAATGCAGCATCTTCATCAGATAATAAGAAAAAATTATCAGAAGTTGCTA CTAAGAAAAATGAAAACGGTTCGACAAAAAATGGTAGTAATGACATCATT GACAAATATGCCAAACTCTTAACAAAATCTAGTTCTTCAACTAGTATGAG AAACGGTAGTTCAGATAGCAATCAACAGAATTTTAAAACAACAGATAATG ATGGTGATTGAACTATTGTTGGTGATGAATTAGGTAAATATAAGTCTAGT GAACTGCCTATTTTTACAGGTAGTTCTTCATACCCAACTTTTCAAACTGA AGCACAAAATGTTTTAGATGGTGGAGCGAATGTTGCTTCAACACAAGGCT TTAAATGAAGCTTTAAACAAATTTAG 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1300 1350 1400 1450 1500 1550 1600 1650 1700 1750 1776
Transcription unit	TU_023
Empirical formula (pH 7.5)	H₁₈₉₆₆C₁₇₁₈₉N₇₁₆₇O₁₂₃₇₇P₁₇₇₆
Molecular weight (pH 7.5; Da)	578987.82

Functional genomics
Is essential	No (Show evidence) Value: False Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	1.29325 (dimensionless) (Show evidence) Value: 1.29325 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	18402300
pI	3.99

Comments
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:11
Last updated	2012-10-01 15:12:43