MG_031_MONOMER – DNA polymerase III, alpha subunit, Gram-positive type

Name
WID	MG_031_MONOMER
Name	DNA polymerase III, alpha subunit, Gram-positive type
Cross references	PDB: 2HQA

Genetics
Gene	MG_031

Structure
Sequence	1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151 1201 1251 1301 1351 1401 1451 MVFNLENEKDKKVLALSNFLIDNNILDHNELNSLIERIESIYFNKYIEEK VFLFAITISRPLTIDIWNALYEGFNELNEGFKADNESFKLTITFKENEPF FKSKNSFSSVTIAIIKDYFHSFFSKDKKYKILIEQELSNPNFLSYENDEL KAQCQTKELTEWLVQKSKSFIFWMNNAGFKNFNFIALYPIDKNESKLKVK AVTVSQYDKQFETKVFATEFIPIHKINQQIDDVKIIGQIFELKTHESLTG KKTLNIYVTDFQLGGSLILKWSYTDEKKIEGITIGNWIKAHIQVERDPNT QILYGIVREINPVEIPNNYKRLDLSKQKRVELVFHTKMTAFDGINDIEEY AQFAKERGWKAITVTDKDNIHIYPKFYEVAKKYDLKAIYGLEFNLTDDHI KIVHNPDNTKLSDATFVIFDIETTGLHGRYDDVIEFSARKIKNNSEIDHQ QFFLKIDKPIPKTITEITKITDEMLEGGIDQQQGLEKIRNYLDDCVMVAH NGINFDLPFLQTQFEKYNIKPLTNPLIDTLCLSWALNPLFSSHTLSNICS KLKLEFDDERLHRAEYDTEALKKVFFYFKKQLKEMGINTLTEIDQNLNKK CQIDLMKRVFTNTAIVYVKNQRGFQNLYEMLSIALTDHNANRPLVLASSL AKFRKSFLLTENPVQGDIFKAALTKPINELEKAIEKVDFVLISQPNAYLG YTLREGLKKELINDAIKLVIKTATKLKKLVAVASDAYFIHPWENEYYKAI VCAKGLGGKWHRHFNNKEKEQTVPEVFLHTTDEMLKRMSFLGEDIAYKLV VENTNKIVKLLDLNELVPTKNKLYPPVMQDSNQKLIDKTWKQAEKRYGKN LPKLIKERIEKELNAIISNGFGIVFWISHLLVEQSVKDGYFVGPRGSIGS SLIANLIGISEINPLAAHYLCEQCHYFEVSDSVDDGYDLMIRDCPKCHEK ASFKGDGHNIPFATFMGFSGDKIPDIDLNFSSEYQAKAHDYVRKLFGVNN TFRAGTIATVAEKTAYGYARNYFEIIKRVDLATTAEIERFKQKLIGIKRT TGQHPGGIMIFPSDHSVYEFTPCGFPADDVESEWKTTHFEYDALGDAILK LDILGQDDPTMLKHLADLTKINPQNIPHFDKNLISMFSSNKPLNLKPGIV DEVTGAVGIPEFGTKFVRKILEQTKPKDFADLIRVSGLSHGKNVWADNAQ KLIKSNRLTLRDVIACRDDIMLYLINKGMQAKDAFEIMEKVRKGIKVNAK EVSLMQNCGVEQYWINACLKINYLFPKAHAAAYVLMAWRIAWFKLYHPLS YYACLLSFKLKEHDINGFEKGYEFIKNRLDELNKLYRIKKIKPKEAELLT SYEVYLEMMARNIKLQQISIQNSNARMFVEHNGVLIAPFITIPGMGEAVA SSIVEARNEKPFASLNDFKKRTKITKKHVETMEQLQLFDEFEHQDDHKLF N* 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1300 1350 1400 1450 1452
Is N‑terminal methionine cleaved	No
Empirical formula (pH 7.5)	H₁₁₉₂₀C₇₆₃₉N₁₉₆₇O₂₁₈₀S₄₁
Molecular weight (pH 7.5; Da)	167508.44
Extinction coefficient (260 nm, 25C, pH 7.0)	170335
Instability index	25.71
Is stable	True
Aliphatic index	92.42
GRAVY (25C, pH 7.0)	-0.331
Half life (OD (600 nm) = 0.3, M9 media, 36C; min)	1200

Synthesis
Localization	c

Function
Complex subunit	DNA-directed DNA polymerase [c]: MG_031_MONOMER + MG_261_MONOMER ⇒ DNA_POLYMERASE_CORE

Comments
Comments	Functional Macromolecule Functional as heterocomplex (see DNA_POLYMERASE_CORE) [PUB_0312, PUB_0313, PUB_0314, PUB_0428]. Complex composition inferred from B. subtilis DNA pol III holoenzyme structure [PUB_0428].
References	Bruck I, O'Donnell M. The DNA replication machine of a gram-positive organism. J Biol Chem 275, 28971-83 (2000). WholeCell: PUB_0314, PubMed: 10878011 Kelman Z, O'Donnell M. DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine. Annu Rev Biochem 64, 171-200 (1995). WholeCell: PUB_0312, PubMed: 7574479 Razin S, Herrmann R. Molecular biology and pathogenicity of mycoplasmas. Springer (2002). WholeCell: PUB_0428, ISBN: 9780306472879 Razin S, Yogev D, Naot Y. Molecular biology and pathogenicity of mycoplasmas. Microbiol Mol Biol Rev 62, 1094-156 (1998). WholeCell: PUB_0313, PubMed: 9841667

Metadata
Created	2012-10-01 15:07:44
Last updated	2012-10-01 15:14:22