MG_025 – Beta-glycosyl transferase

Name
WID	MG_025
Name	Beta-glycosyl transferase
Symbol	trsB
Protein product	MG_025_MONOMER
Cross references	GenBank: AAC71241.1, CMR: MG_025, BioCyc: MG_025, SwissProt: P47271
Homologs	B. subtilis: BSU35680 M. pneumoniae: MPN_028 S. oneidensis: SO_3180

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 28652 (nt), Length: 897 (nt), Direction: Forward, G/C content: 29.1%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 GTGCCAAGTAAGTATTTATTCACCGTTATAATTCCAACATACAATTGTTG TCAATACATAAAAAAGGCATTAGATTCATTGCTTTTACAGAATGAATATT TTTTAAAAACACAAGTACTAATAGTTAACGATGGTTCACTAGACAATACT AAGGAAGTTGTTAGTGACTATTTAATAAAGTACTCCAATATTAGTTATTT TGAAAAAACTAATGGTAATTGGGGTAGTGTTATTAACTATGTTAAAAAAA ATAAATTAGCCTTAGGTCAATATATTACTGTTTTAGATAGCGATGATTAT TTTTTAAAAGATAGTTTTAAAAAAGTGGCTCGTTTCTTTGGCCATGACAT GATCATTGGCGCTTTTTATTGTTATATTAATGAAAATAAAACTCGTTTTT TAAAACCTTATTTCGGTAAGACTGGTGTTATTAAAGAACATACCAAATTA AGAACCCCCCATTCCCAACCTATTGCTAAATTTTACAGTAATAAACTTTT CTATGAACTACATGATCTTAAGGAAAAGTTATTCTTCCAGGATTGTTTAA TGTATCACGATGCTATTAACAGAGTTGAAAGTGTTTTTTATTTGCGAGAG CCTTTAGCAGTTTGGTTTTCTACAAGACCTGGCAATTCTACAACAACTTC TTGAGAAAATCCAAATAAATTTAATGCTTGGTGTGAAATTCTCCAAAAAA TGAATTTGTATGGAGCTGGGATAGTAATCTACATCTATACTATGCTACCT GGATTTCTAAAACAACTAAAGAAAAAACAACTAATACTGAATTTGAACCA TAAACCAGCTTACACTTGATTACCTAAACCTTTAGCGTTTATTTTTGGTG GTTTAATGGCATTTAAAACCAGAAAATACATTAAATATCCTAAGTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 897
Transcription unit	TU_012
Empirical formula (pH 7.5)	H₉₅₅₅C₈₆₅₇N₃₅₄₆O₆₂₇₀P₈₉₇
Molecular weight (pH 7.5; Da)	291374.61

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.560583 (dimensionless) (Show evidence) Value: 0.560583 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	9310100
pI	4.09

Comments
Comments	Synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein, for example: (malQ, coli): (glucose)n + (glucose)m (glucose)n+m-1 + alpha-D-glucose [PUB_0110]; LPS O:3 antigen (homopolymer of 6-deoxy-L-altrose repeating units linked by 1,2 linkages) biosynthesis; NDP-sugar hexosyltransferases; group 4 nrd2 glycosyltransferases: cell surface polysaccharide (CSP) biosynthesis [PUB_0238]; Suthers et al [PUB_0610] annotates MG_025 as a minor teichoic acid synthase. However evidence for this annotation is weak.
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Chen J, Anderson JB, DeWeese-Scott C, Fedorova ND, Geer LY, He S, Hurwitz DI, Jackson JD, Jacobs AR, Lanczycki CJ, Liebert CA, Liu C, Madej T, Marchler-Bauer A, Marchler GH, Mazumder R, Nikolskaya AN, Rao BS, Panchenko AR, Shoemaker BA, Simonyan V, Song JS, Thiessen PA, Vasudevan S, Wang Y, Yamashita RA, Yin JJ, Bryant SH. MMDB: Entrez's 3D-structure database. Nucleic Acids Res 31, 474-7 (2003). WholeCell: PUB_0110, PubMed: 12520055, URL: http://www.ncbi.nlm.nih.gov/Structure/ Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Kapitonov D, Yu RK. Conserved domains of glycosyltransferases. Glycobiology 9, 961-78 (1999). WholeCell: PUB_0238, PubMed: 10521532 Suthers PF, Dasika MS, Kumar VS, Denisov G, Glass JI, Maranas CD. A genome-scale metabolic reconstruction of Mycoplasma genitalium, iPS189. PLoS Comput Biol 5, e1000285 (2009). WholeCell: PUB_0610, PubMed: 19214212 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:10
Last updated	2012-10-01 15:12:41