MG_022_MONOMER – DNA-directed RNA polymerase, delta subunit

Name
WID MG_022_MONOMER View in model
Name DNA-directed RNA polymerase, delta subunit View in model
 
Genetics
Gene MG_022 View in model
 
Structure
Sequence
1
51
101
MQLEYLNLISQAKVIAEKQFKANPFSFETIWKEVVKHFKISKQDEPSLIG
RFYQDFLEDPNFVYLGDRKWKLRDFMKFDEWNKISQSMFVTKEIFEEGYE
DLSNKKVEPEEGVGDFIMGNDGDDNETGSEIVQGLINDSFSEENQ*
50
100
146
 View in model
Is N‑terminal methionine cleaved
No
 View in model
Empirical formula (pH 7.5) H1143C772N190O240S4
Molecular weight (pH 7.5; Da) 17053.68 View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)		 22460
Instability index 36.01
Is stable True
Aliphatic index 68.70
GRAVY (25C, pH 7.0) -0.696
Half life (OD (600 nm) = 0.3, 
M9 media, 36C; min)		 1200 View in model
 
Synthesis
Localization c View in model
 
Function
Complex subunit DNA-directed RNA polymerase
[c]: MG_022_MONOMER + (2) MG_177_MONOMER + MG_340_MONOMER + MG_341_MONOMER ⇒ RNA_POLYMERASE		 View in model
 
Comments
Comments Functional Macromolecule Functional as heterocomplex (see RNA_POLYMERASE). Crystal structure available of Thermus thermophilus RNA polymerase [PUB_0430]. Gram positive bacteria such as mycoplasmas contain additional delta factor beyond gram negative RNA polymerase [PUB_0423]. RNA polymerase holoenzyme has structure β'βα2δσ [PUB_0355].
References

  1. Achberger EC, Whiteley HR. The role of the delta peptide of the Bacillus subtilis RNA polymerase in promoter selection. J Biol Chem 256, 7424-32 (1981). WholeCell: PUB_0423, PubMed: 6788769

  2. Bordier C, Rossetti GP. Subunit composition of Escherichia coli RNA polymerase during transcription in vitro. Eur J Biochem 65, 147-53 (1976). WholeCell: PUB_0355, PubMed: 776624

  3. Vassylyev DG, Sekine S, Laptenko O, Lee J, Vassylyeva MN, Borukhov S, Yokoyama S. Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 A resolution. Nature 417, 712-9 (2002). WholeCell: PUB_0430, PubMed: 12000971
 
Metadata
Created 2012-10-01 15:07:44
Last updated 2012-10-01 15:14:21