MG_022 – DNA-directed RNA polymerase, delta subunit

Name
WID	MG_022
Name	DNA-directed RNA polymerase, delta subunit
Symbol	rpoE
Protein product	MG_022_MONOMER
Cross references	GenBank: AAC71238.1, CMR: MG_022, BioCyc: MG_022, SwissProt: P47268
Homologs	M. pneumoniae: MPN_024

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 26036 (nt), Length: 438 (nt), Direction: Forward, G/C content: 30.4%, Sequence: 1 51 101 151 201 251 301 351 401 ATGCAATTAGAGTACCTCAATCTTATTTCTCAAGCTAAAGTTATTGCAGA AAAACAATTTAAAGCTAACCCTTTTTCTTTTGAAACAATTTGAAAAGAAG TAGTTAAACATTTCAAGATTTCAAAACAAGATGAACCAAGCTTAATTGGT CGTTTTTATCAAGATTTTCTTGAGGATCCTAACTTTGTCTATTTAGGTGA TAGAAAATGAAAACTTCGTGATTTTATGAAGTTTGATGAATGGAACAAGA TATCACAATCTATGTTTGTTACAAAGGAGATTTTTGAAGAAGGTTATGAA GATCTTTCCAATAAAAAAGTAGAACCTGAGGAAGGAGTTGGTGATTTCAT TATGGGAAATGATGGTGATGACAATGAAACTGGCAGTGAAATAGTACAAG GTTTAATTAATGATTCATTCAGTGAGGAAAATCAATAG 50 100 150 200 250 300 350 400 438
Transcription unit	TU_011
Empirical formula (pH 7.5)	H₄₆₇₇C₄₂₃₈N₁₇₆₄O₃₀₄₆P₄₃₈
Molecular weight (pH 7.5; Da)	142623.81

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	5.89708 (dimensionless) (Show evidence) Value: 5.89708 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	4587100
pI	3.99

Comments
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:10
Last updated	2012-10-01 15:12:40