MG_020 – proline iminopeptidase

Name
WID	MG_020
Name	proline iminopeptidase
Symbol	pepP
Synonyms	pip
Protein product	MG_020_MONOMER
Cross references	GenBank: AAC71236.1, CMR: MG_020, BioCyc: MG_020, SwissProt: P47266
Homologs	M. pneumoniae: MPN_022 S. coelicolor: SCO1989 S. oneidensis: SO_4400

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 23542 (nt), Length: 927 (nt), Direction: Forward, G/C content: 30.2%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 ATGAATACAAAACTTAATGTAAAAGGTTATCTAAATGTTGGTGATAACCA TCAACTTTATTACTGAACACAAGGAAATCCTAATGGTAAACCGGTTTTGT ATATCCATGGCGGACCTGGTTCTGGTACTGATGAAGGATGTTTAAAGTAT TTTGATCTTGAAACAACTTGGATTATTTTATTAGATCAAAGAGGTTGTGG TAAGAGTAAGACTAATGATATCTTTTATGAAAATAACACTGATAAATTAG TTAGTGATTTTGAAATTTTACGTCAAAAATTAAACATTAAAAACTGAACA CTCTTTGGTGGTAGTTGGGGTTCTGCACTTGCTTTAGTTTATGCAATTAA ACACCCACAAGTAGTTGATAAGATCTTTTTAAGAGCACTTTTTTTAGCTA GAGAAAAAGACTGATCTGAAGCTTTAATGGGATTAGGAAAAATGTTTTAT CCTTATGAACACCAACGCTTTATGGATAGTATTCCTAAAGCTTATCAGAA CAGTTATGAACAAATTGTTAACTACTGTTATGATCAATTTCAAAATGGTG ATGAATCAACCAAAGAAAAACTTGCTAAAGCTTGGGTGGATTGAGAATCA ACATTACTTTCACCTATTAACAAAATTCATTCAACAGCAACAGACTTTAA ATTAGTTGAAAAACTAGCTTTATTGGAATGTCACTATGCAGTTAATAAAA GTTTTTTGGATGAAAACTTCATTCTAGATAACATTAGTGTTCTTAAAAAT AAAAGTATTTATTTGGCTCATGGTAGATTTGATCTGATCTGTCCTTTATA TCAACCATTAGCATTAAAACAAGCATTCCCTGAATTACAACTTTATGTAA CCAATAATGCTGGTCATAGTGGTAGTGATGCTAATAATTTAGCAACTATA AAACACCTTTTAAAAACTTACCTTTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 927
Transcription unit	TU_010
Empirical formula (pH 7.5)	H₉₈₈₄C₈₉₅₆N₃₆₉₃O₆₄₇₁P₉₂₇
Molecular weight (pH 7.5; Da)	301501.49

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	1.29325 (dimensionless) (Show evidence) Value: 1.29325 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	9615500
pI	4.06

Comments
Comments	prolyl oligopeptidase; a/b-hydrolase fold family enzyme [PUB_0032]; cytosol aminopeptidase V [PUB_0033]; removes N-terminal amino acid, preference proline >> alanine >> other amino acids [PUB_0032]
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Holmquist M. Alpha/Beta-hydrolase fold enzymes: structures, functions and mechanisms. Curr Protein Pept Sci 1, 209-35 (2000). WholeCell: PUB_0032, PubMed: 12369917 Walter R, Simmons WH, Yoshimoto T. Proline specific endo- and exopeptidases. Mol Cell Biochem 30, 111-27 (1980). WholeCell: PUB_0033, PubMed: 6991912 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:10
Last updated	2012-10-01 15:12:40