MG_010 – DNA primase-related protein

Name
WID	MG_010
Name	DNA primase-related protein
Symbol	dnaE
Protein product	MG_010_MONOMER
Cross references	GenBank: AAC71226.1, CMR: MG_010, BioCyc: MG_010, SwissProt: P47256
Homologs	M. hyopneumoniae: mhp062 M. mobile: mhp062 M. pneumoniae: MPN_014

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 12068 (nt), Length: 657 (nt), Direction: Forward, G/C content: 26.5%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 ATGGATGTTAGAACTGAAAGATTAAACGAATTGTTTTTTGTTTATCATAA AAACTTAAAAAATCAATCTAAATCTAAATATAGCAGAGCAGTTAATTACT TAAAAAGGCGTGGATTCAATTTACAAGATTTTTTAAAAGTTGGGGGTGGT GTTGGTTATTTACAAAATAAAGAATGATTAAATTTACCTTTATACAGTTT TGATGGTAATTTAATTGGTTTTCTAAACAGAAAAGTTAGTTATAAAAAGG AATTTTTATATACACCTTTTAATAAACCTCCTTCAAAGAGCGAAGCTTTT GTAGGACTCAGAGAATTAGTTATTAAAGACAATAGCATATATCTTGTTGA AGGTGATTTTGACTGGTTAGCTTTTCGCAAAGCAGGTATATTAAATTCTC TTCCTCTTTGTGGTTTGACTATTTCAAATCAACAAGTTCAATGATTAAAA CAAAAAAAGATTAAAAAGATTTTTATCTGTTTTGATAATGATTTAGCTGG AAAGAATGGAGCAAAAAATTTAAAAGAATATCTAACTAAGCAAGGATTTA TAACAAAAGTTATAGAAATTAAAGCTGCCGCAAAAGACTGAAATGATTTG TTTTTATTAAACAACTCAAATTGATCAGCGGTTTTAACTAATCAACTTCT TTTTTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 657
Transcription unit	TU_004
Empirical formula (pH 7.5)	H₆₉₉₇C₆₃₃₉N₂₅₉₂O₄₅₇₉P₆₅₇
Molecular weight (pH 7.5; Da)	213104.51

Functional genomics
Is essential	No (Show evidence) Value: False Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.242995 (dimensionless) (Show evidence) Value: 0.242995 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	6907800
pI	4.09

Comments
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:10
Last updated	2012-10-01 15:12:39