MG_0004 – transfer-messenger RNA, tmRNA, MCS6, 10Sa RNA
Name | ||
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WID | MG_0004 |
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Name | transfer-messenger RNA, tmRNA, MCS6, 10Sa RNA |
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Symbol | ssrA | |
Cross references | CMR: MG_0004 | |
Classification | ||
Type | sRNA |
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Structure | ||
Structure |
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Sequence | Chromosome: Mgenitalium_Chr_1, Coordinate: 406542 (nt), Length: 388 (nt), Direction: Reverse, G/C content: 37.1%, Sequence: 1 51 101 151 201 251 301 351 TGGTGGAGATGACGGGAATCGAACCCGTTTCCATAAAGGACACAACAAAC AGCTTCTACATTTTAGTTTTGTGGATTTTTGTCTTGTTTCCAATTGATCA CAAAACAATCATGGAAACAACAAGTTCTATTTTTAAAGGTTAAATTGAAC GATTTAACCTCGAGTCATAAACTATGACAGCTAGCAAACTATGACTATTC ACCAGCTGTAGCTTTAGTATCTACTTATGCAAAAGCAAAGTTAACACTTG CTTGTTGGTTAATGATCAAATTTGGATCTACCAACACTTCGTTATTTTCT TTGTCGGTTATTGAACCTAGCGCTGTAAGGGGCATACCCCAATGCTACTG TTTGTCTATCAGCATTATGTCAAACCCAAAACATCCCC 50 100 150 200 250 300 350 388 |
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Transcription unit | TU_227 |
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Empirical formula (pH 7.5) | H4145C3756N1568O2721P388 | |
Molecular weight (pH 7.5; Da) | 126804.64 |
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Functional genomics | ||
Is essential | Yes (Show evidence) |
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Relative expression | 147.374 (dimensionless) (Show evidence) |
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Half life | 89.0 (min) (Show evidence) |
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Amino acid | ALA |
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Extinction coefficient (260 nm, 25C, pH 7.0) |
3893500 | |
pI | 4.03 | |
Comments | ||
Comments | Has tRNA-like structure with an mRNA-like domain. The tRNA-like domain is charged by alanyl tRNA synthetase and with the help of SmpB (MG_059_MONOMER) replaces the tRNA of stalled ribosomes (ribosomes stalled for example due to mRNA damage, mRNA lacking a stop codon, rare codons, or natural stall sequences), adding an alanine to the stalled peptide. Next the mRNA-like domain is translated by the ribosome add the C-terminal amino acid sequence DKENNEVLVEPNLIINQQASVNFAFA to the stalled peptide [PUB_0020]. The added C-terminal tag marks the peptide for degradation [PUB_0014]. MG_0004 has a half-life of 89 min [PUB_0396]. The expression of MG_0004 is 5% that of 5S RNA [PUB_0400]. | |
References |
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Metadata | ||
Created | 2012-10-01 15:07:09 | |
Last updated | 2012-10-01 15:12:37 |