MG_0003 – Rnase P RNA, MCS5, M1 RNA

Name
WID	MG_0003
Name	Rnase P RNA, MCS5, M1 RNA
Symbol	rpnB
Cross references	CMR: MG_0003

Classification
Type	sRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 406142 (nt), Length: 378 (nt), Direction: Reverse, G/C content: 35.7%, Sequence: 1 51 101 151 201 251 301 351 GCTGTCGGTTAGTTGCTGCTTTAAAGATATATTTAAAGTAGAGGAAAGTC CATGCTTGCACTTGCTGGAATGCAAGTAGTGTTTGTGTAAATCAAAGCGA TAAGATTTAGGATGTGATAGCCATAACGACATAACTAGTCTAAGGTTAAT ACTATGATGATGTTTTGAAAGTGCCACAGAGACGAGCTTGGTGAAAACCA GGTATGAAACGCGGTAAACTCCACAAGCAAGAAACCTAAATTTTGGTAAG GGAATGTTGCTTTAAGAACAAATAATTAAAGTAATGACAGCAATTGCTGT AGATAAATAACTGACTTTAAAACAGAACATGGCTTATTGACAGCATGCTA TCTAAAAAAAGGATCATGATGATCCTTT 50 100 150 200 250 300 350 378
Transcription unit	TU_226
Empirical formula (pH 7.5)	H₄₀₅₃C₃₆₇₄N₁₅₇₂O₂₆₁₆P₃₇₈
Molecular weight (pH 7.5; Da)	123793.39

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	442.124 (dimensionless) (Show evidence) Value: 442.124 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	1200.0 (min) (Show evidence) Value: 1200 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	3917200
pI	3.80

Function
Complex subunit	ribonuclease P [c]: MG_0003 + MG_465_MONOMER ⇒ MG_0003_465

Comments
Comments	Peters et al performed a phylogenic analysis of RNaseP from several haemoplasma and Mycoplasma species [PUB_0823].
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Peters IR, Helps CR, McAuliffe L, Neimark H, Lappin MR, Gruffydd-Jones TJ, Day MJ, Hoelzle LE, Willi B, Meli M, Hofmann-Lehmann R, Tasker S. RNase P RNA gene (rnpB) phylogeny of Hemoplasmas and other Mycoplasma species. J Clin Microbiol 46, 1873-7 (2008). WholeCell: PUB_0823, PubMed: 18337389 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:09
Last updated	2012-10-01 15:12:37