MG514_Aminoacylation – asparaginyl-tRNA (MG514) aminoacylation

Name
WID	MG514_Aminoacylation
Name	asparaginyl-tRNA (MG514) aminoacylation
Cross references	EC: 6.1.1.22, BioCyc: ASPARAGINE--TRNA-LIGASE-RXN

Classification
Type	aminoacylation

Reaction
Stoichiometry	[c]: ASN + ATP ⇒ AMP + PPI
Modification	MG514 [c]

Catalysis
Enzyme	MG_113_DIMER [c]

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False

Kinetics
Forward kinetics	V_max = 1620.0 1/min (Show evidence) Value: Rate law: VmaxASN/(Km+ASN); Km = 0.12 mM, Vmax = 0.566 umol/(minmg) (None) Measured: Yes Species: Phaseolus aureus References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Process	Process_tRNAAminoacylation

Comments
Comments	wt yeast from SABIO-RK
References	Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:50
Last updated	2012-10-01 15:17:20