MG501_Aminoacylation – lysyl-tRNA (MG501) aminoacylation

Name
WID	MG501_Aminoacylation
Name	lysyl-tRNA (MG501) aminoacylation
Cross references	EC: 6.1.1.6, BioCyc: LYSINE--TRNA-LIGASE-RXN

Classification
Type	aminoacylation

Reaction
Stoichiometry	[c]: ATP + LYS ⇒ AMP + PPI
Modification	MG501 [c]

Catalysis
Enzyme	MG_136_DIMER [c]

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False

Kinetics
Forward kinetics	V_max = 456.0 1/min (Show evidence) Value: Rate law: VmaxLYSATP/(Km1Km2+Km1ATP+Km2LYS); Km = 0.006 mM, 0.33 mM, Vmax = 405 umol/(minmg) (None) Measured: Yes Species: Escherichia coli References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Process	Process_tRNAAminoacylation

Comments
References	Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:48
Last updated	2012-10-01 15:17:13