MG490_Aminoacylation – phenylalanyl-tRNA (MG490) aminoacylation

Name
WID	MG490_Aminoacylation
Name	phenylalanyl-tRNA (MG490) aminoacylation
Cross references	EC: 6.1.1.20, BioCyc: PHENYLALANINE--TRNA-LIGASE-RXN

Classification
Type	aminoacylation

Reaction
Stoichiometry	[c]: ATP + PHE ⇒ AMP + PPI
Modification	MG490 [c]

Catalysis
Enzyme	MG_194_195_TETRAMER [c]

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False

Kinetics
Forward kinetics	V_max = 324.0 1/min (Show evidence) Value: Rate law: VmaxPHEATP/(Km1Km2+Km1ATP+Km2PHE); Km = 0.0012 mM, 0.147 mM, Vmax = 0.074 umol/(minmg) (None) Measured: Yes Species: Staphylococcus aureus References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Process	Process_tRNAAminoacylation

Comments
Comments	bhandary tRNA book
References	Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:47
Last updated	2012-10-01 15:17:09