MG472_Aminoacylation – isoleucyl-tRNA (MG472) aminoacylation

Name
WID	MG472_Aminoacylation
Name	isoleucyl-tRNA (MG472) aminoacylation
Cross references	EC: 6.1.1.5, BioCyc: ISOLEUCINE--TRNA-LIGASE-RXN

Classification
Type	aminoacylation

Reaction
Stoichiometry	[c]: ATP + ILE ⇒ AMP + PPI
Modification	MG472 [c]

Catalysis
Enzyme	MG_345_MONOMER [c]

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False

Kinetics
Forward kinetics	V_max = 255.762 1/min (Show evidence) Value: Rate law: VmaxILEATP/(Km1ATP+Km2ILI+ILEATP); Km = 10 uM, 330 uM, Vmax = 4.2627 1/sec (None) Measured: Yes* Species: Pseudomonas fluorescens References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Process	Process_tRNAAminoacylation

Comments
Comments	wt Pseudomonas Fluorescens from SABIO-RK
References	Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:45
Last updated	2012-10-01 15:17:04