LdhA – D-lactate dehydrogenase

Name
WID	LdhA
Name	D-lactate dehydrogenase
Cross references	EC: 1.1.1.28, SABIO-RK: 16559, BioCyc: L-LACTATE-DEHYDROGENASE-RXN, BiGG: LDH_D, KEGG: R00703

Classification
Type	chemical

Reaction
Stoichiometry	[c]: H + NADH + PYR ⇔ LAC + NAD

Catalysis
Enzyme	MG_460_TETRAMER [c]
Optimal pH	7.1 (dimensionless)
Optimal temperature	44.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	6.10
K_eq	3.97912485152e-05 ()

Kinetics
Forward kinetics	v = V_max * [PYR] * [NADH] / (K_m1 * K_m2 + K_m1 * [NADH] + K_m2 * [PYR]); K_m1 = 660 (nM), K_m2 = 45000 (nM), V_max = 2000.0 U/mg
Backward kinetics	(Show evidence) Value: Rate law: VmaxPYR/(Km+PYR); Km = 0.66 mM, Vmax = 2000 µmol/(minmg) (None) Measured: Yes Species: Lactobacillus curvatus References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Pathways	PYRUVATE_METABOLISM
Process	Process_Metabolism

Comments
References	Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:38
Last updated	2012-10-01 15:16:45