HemG – protoporphyrinogen oxidase (aerobic)

Name
WID	HemG
Name	protoporphyrinogen oxidase (aerobic)
Cross references	EC: 1.3.3.4, BiGG: PPPGO, KEGG: R03222

Classification
Type	chemical

Reaction
Stoichiometry	[c]: (3) O2 + PPHG ⇒ (3) H2O2 + PPIX

Catalysis
Enzyme	MG_259_TETRAMER [c]
Coenzymes	FAD [c] (Show evidence) Value: FAD Measured: Yes References Chang A, Scheer M, Grote A, Schomburg I, Schomburg D. BRENDA, AMENDA and FRENDA the enzyme information system: new content and tools in 2009. Nucleic Acids Res 37, D588-92 (2009). WholeCell: PUB_0570, PubMed: 18984617 Value: FMN Measured: Yes Species: Escherichia coli K12 References Keseler IM, Bonavides-Martínez C, Collado-Vides J, Gama-Castro S, Gunsalus RP, Johnson DA, Krummenacker M, Nolan LM, Paley S, Paulsen IT, Peralta-Gil M, Santos-Zavaleta A, Shearer AG, Karp PD. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res 37, D464-70 (2009). WholeCell: PUB_0006, PubMed: 18974181
Optimal pH	7.8 (dimensionless)
Optimal temperature	24.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False

Kinetics
Forward kinetics	v = V_max * [PPHG] / (K_m + [PPHG]); K_m = 850 (nM), V_max = 7.15 U/mg (Show evidence) Value: Rate law: VmaxPPHG/(Km+PPHG); Km = 0.85 mM, Vmax = 7.15 umol/(minmg) (None) Measured: Yes Species: Homo sapiens References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Pathways	COFACTOR_PROSTHETIC_BIOSYNTHESIS
Process	Process_Metabolism

Comments
References	Chang A, Scheer M, Grote A, Schomburg I, Schomburg D. BRENDA, AMENDA and FRENDA the enzyme information system: new content and tools in 2009. Nucleic Acids Res 37, D588-92 (2009). WholeCell: PUB_0570, PubMed: 18984617 Keseler IM, Bonavides-Martínez C, Collado-Vides J, Gama-Castro S, Gunsalus RP, Johnson DA, Krummenacker M, Nolan LM, Paley S, Paulsen IT, Peralta-Gil M, Santos-Zavaleta A, Shearer AG, Karp PD. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res 37, D464-70 (2009). WholeCell: PUB_0006, PubMed: 18974181 Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:38
Last updated	2012-10-01 15:16:43