HR_strandExchange – DNA recombination strand exchange

Name
WID HR_strandExchange View in model
Name DNA recombination strand exchange View in model
 
Classification
Type homologous_recombination View in model
 
Reaction
Stoichiometry [c]: ATP + H2O ⇒ ADP + H + PI View in model
 
Catalysis
Enzyme MG_339_MONOMER [c] View in model
 
Energetics
Is spontaneous (pH 7.5, 25C, I = 0) False View in model
 
Kinetics
Forward kinetics Km = 28.37 (nM), Vmax = 3.6 1/min View in model
 
Associations
Process Process_DNARepair View in model
 
Comments
Comments RecA promotes ATP-dependent strand exchange between ssDNA and dsDNA [PUB_0529]. RecA effects a 106-fold rate enhancement [PUB_0529]. It is not yet clear how RecA protein-mediated ATP hydrolysis is coupled to strand exchange [PUB_0529]. Kinetic rate determined by Bazemore et al (0.05-0.07 1/s) [PUB_0528], Xiao et al (0.053-0.42) [PUB_0529], and Bhattacharyya et al (0.082 1/min) [PUB_0530]. Km determined by Bhattacharyya et al (28.37 μM) [PUB_0530].
References

  1. Bazemore LR, Takahashi M, Radding CM. Kinetic analysis of pairing and strand exchange catalyzed by RecA. Detection by fluorescence energy transfer. J Biol Chem 272, 14672-82 (1997). WholeCell: PUB_0528, PubMed: 9169430

  2. Bhattacharyya MK, Bhattacharyya nee Deb S, Jayabalasingham B, Kumar N. Characterization of kinetics of DNA strand-exchange and ATP hydrolysis activities of recombinant PfRad51, a Plasmodium falciparum recombinase. Mol Biochem Parasitol 139, 33-9 (2005). WholeCell: PUB_0530, PubMed: 15610817

  3. Xiao J, Lee AM, Singleton SF. Direct evaluation of a kinetic model for RecA-mediated DNA-strand exchange: the importance of nucleic acid dynamics and entropy during homologous genetic recombination. Chembiochem 7, 1265-78 (2006). WholeCell: PUB_0529, PubMed: 16847846
 
Metadata
Created 2012-10-01 15:08:38
Last updated 2012-10-01 15:16:44