HR_DNAResection_dGMP – DNA resection (dGMP)

Name
WID HR_DNAResection_dGMP View in model
Name DNA resection (dGMP) View in model
 
Classification
Type homologous_recombination View in model
 
Reaction
Stoichiometry [c]: dGpdGp + H2O ⇒ (2) DGMP + H View in model
 
Catalysis
Enzyme MG_262_MONOMER [c] View in model
 
Energetics
Is spontaneous (pH 7.5, 25C, I = 0) False View in model
 
Kinetics
Forward kinetics Km = 0.1 (nM), Vmax = 6.6 1/min View in model
 
Associations
Process Process_DNARepair View in model
 
Comments
Comments Homologous recombination is most efficient with overhang lengths of at least 8 bases [PUB_0526]. Kinetics observed for S. pneumoniae PolI by Amblar and Lopez [PUB_0527].
References

  1. Amblar M, López P. Purification and properties of the 5'-3' exonuclease D190-->a mutant of DNA polymerase I from Streptococcus pneumoniae. Eur J Biochem 252, 124-32 (1998). WholeCell: PUB_0527, PubMed: 9523721

  2. Daley JM, Wilson TE. Rejoining of DNA double-strand breaks as a function of overhang length. Mol Cell Biol 25, 896-906 (2005). WholeCell: PUB_0526, PubMed: 15657419
 
Metadata
Created 2012-10-01 15:08:38
Last updated 2012-10-01 15:16:44