H2O – water

Name
WID	H2O
Name	water
Traditional name	((18)O)water
IUPAC name	water
Cross references	ChEBI: 15377, PubChem: 3303, CAS: 7732-18-5, ThreeDMET: B01124, KEGG: C00001, ... 3 moreBiGG: h2o, PDBCCD: HOH, BioCyc: WATER

Structure
Empirical formula (pH 7.5)	H₂O₁
SMILES (pH 7.5)	[OH2]
Charge (pH 7.5)	0
Is hydrophobic	False
Molecular weight (Da)	18.02
van der Waals volume (pH 7.5; Å³ molecule^‑1)	19.51
Δ_fG^'o (pH 7.5, 25C, I = 0; kJ mol^‑1)	-150.40
pI	6.96
logP	-0.850
logD (pH 7.5)	-0.850

Concentrations
Media composition (SP4; mM)	54579.20

Function
Complex subunit	cell division protein ftsZ deactivated 9mer [c]: (9) H2O + MG_224_9MER_GTP ⇒ (9) H + (9) PI + MG_224_9MER_GDP
Reaction participant	Protein degradation (ALA) [c]: AlaAla + ATP + (2) H2O ⇒ ADP + (2) ALA + H + PI FtsZ deactivation [c]: H2O + MG_224_MONOMER_GTP ⇔ H + MG_224_MONOMER_GDP + PI Ribosome assembly (RbfA) [c]: GTP + H2O ⇒ GDP + H + PI 2',3'-cyclic-nucleotide phosphodiesterase (UMP) [c]: H2O + U23CMP ⇒ H + U3MP hydroxyl radical generation gamma_radiation[e] + (2) H2O[c] ⇒ (2) H[c] + hydroxyl_radical[c] + OH[c] ... 488 more Peptidyl-tRNA hydrolysis (SER) [c]: AMP_SER + H2O ⇒ AMP + SER Triacylglycerol lipase (dibutyrin) (MG_327) [e]: DIBUTYRIN + (2) H2O ⇔ (2) BUT + GL 2-aminoethylphosphonate aminotransferase [c]: AEPP + H2O ⇒ (2) H + NH3 + PPAA arginine deiminase [c]: ARG + H2O ⇔ CITR + H + NH3 3',5'-bisphosphate nucleotidase [c]: H2O + PAP ⇒ AMP + PI Chromosome segregation (CobQ) [c]: GTP + H2O ⇒ GDP + H + PI Triacylglycerol lipase (1-monobutyrin) (MG_327) [e]: H2O + MONOBUTYRIN ⇔ BUT + GL 5'-nucleotidase (5,6-dihydro-2'-deoxythymidine monophosphate) [c]: DHdTMP + H2O ⇒ DHdT + PI 5'-nucleotidase (2'-O-ethyl-2'-deoxycytidine monophosphate) [c]: H2O + O2edCMP ⇒ O2edC + PI Chromosome segregation (MraZ) [c]: GTP + H2O ⇒ GDP + H + PI phosphoenolpyruvate carboxylase [c]: CO2 + H2O + PEP ⇒ H + OA + PI oligopeptide (His) transport via ABC system ATP[c] + H2O[c] + HisHis[e] ⇒ ADP[c] + H[c] + HisHis[c] + PI[c] Protein degradation (THR) [c]: ATP + (2) H2O + ThrThr ⇒ ADP + H + PI + (2) THR 5'-nucleotidase (2'-deoxycytidine monophosphate-[6,4]-2'-deoxythymidine monophosphate dewar product) [c]: dCMP64dTMP_dewar + (2) H2O ⇒ dC64dT_dewar + (2) PI base excision (5-hydroxyuracil) [c]: H2O + ho5dUMP ⇒ DR5P + ho5URA Protein polymerization (Leu) [c]: (2) LEU ⇒ H2O + LeuLeu alpha-L-glutamate ligase [c]: (4) ATP + (4) GLU + (4) H2O ⇒ (4) ADP + (4) H + (4) H2O + (4) PI 5'-nucleotidase (1-methyl-2'-deoxyadenosine monophosphate) [c]: H2O + m1dAMP ⇒ m1dA + PI Protein degradation (ALA) [c]: AlaAla + ATP + (2) H2O ⇒ ADP + (2) ALA + H + PI Dipeptide import (Val-Val) [c]: H2O + ValVal ⇒ (2) VAL oligopeptide (Trp) transport via ABC system ATP[c] + H2O[c] + TrpTrp[e] ⇒ ADP[c] + H[c] + PI[c] + TrpTrp[c] Protein polymerization (His) [c]: (2) HIS ⇒ H2O + HisHis 5'-nucleotidase (2'-deoxycytidine monophosphate-[6,4]-2'-deoxycytidine monophosphate dewar product) [c]: dCMP64dCMP_dewar + (2) H2O ⇒ dC64dC_dewar + (2) PI Spontaneous base deamination (cytosine ==> uracil) [c]: CSN + H2O ⇒ NH3 + URA 5'-nucleotidase (2-hydroxy-2'-deoxyadenosine monophosphate) [c]: H2O + oxo2dAMP ⇒ oxo2dA + PI glycerol transport via ABC system ATP[c] + GL[e] + H2O[c] ⇒ ADP[c] + GL[c] + H[c] + PI[c] N-terminal methionine cleavage [c]: H2O + MetMet ⇒ (2) MET glycosyltransferase (Phosphatidylglycerol (dihexadec-9-enoyl, n-C16:1) ==> Diacylglycerol (n-16:1)) H2O[c] + PG161[m] ⇒ DAG161[m] + GL3P[c] RNA cleavage (CMP) [c]: CpCp + H2O ⇒ (2) CMP + H 5'-nucleotidase (4'-O-ethyl-2'-deoxythymidine monophosphate) [c]: H2O + O4edTMP ⇒ O4edT + PI 5'-nucleotidase (3'-methyl-2'-deoxythymidine monophosphate) [c]: H2O + m3dTMP ⇒ m3dT + PI DNA radiation (gamma-ray) induced base oxidation (5,6-dihydroxyuracil; dialuric acid ==> 5-hydroxyhydantoin; hydroxyl radical) DHURA[c] + gamma_radiation[e] + H2O[c] ⇒ CO2[c] + (2) H[c] + ho5hydantoin[c] ribonucleoside-diphosphate reductase (ADP) [c]: (2) ADP + MG_229_231_TETRAMER + O2 ⇒ (2) DADP + (2) H2O + MG_229_231_TETRAMER_ox Protein degradation (CYS) [c]: ATP + CysCys + (2) H2O ⇒ ADP + (2) CYS + H + PI L-methionine-R-sulfoxide reductase [c]: H2O + MET + MG_124_MONOMER_ox ⇔ METSOXRL + MG_124_MONOMER ribonucleoside-diphosphate reductase (GDP) [c]: (2) GDP + MG_229_231_TETRAMER + O2 ⇒ (2) DGDP + (2) H2O + MG_229_231_TETRAMER_ox RNA cleavage (AMP) [c]: ApAp + H2O ⇒ (2) AMP + H Protein degradation (PHE) [c]: ATP + (2) H2O + PhePhe ⇒ ADP + H + (2) PHE + PI Peptidyl-tRNA hydrolysis (ILE) [c]: AMP_ILE + H2O ⇒ AMP + ILE tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER Water transport H2O[e] ⇔ H2O[c] 5'-nucleotidase (7-ethyl-2'-deoxyguanosine monophosphate) [c]: e7dGMP + H2O ⇒ e7dG + PI Protein degradation (GLN) [c]: ATP + GlnGln + (2) H2O ⇒ ADP + (2) GLN + H + PI phosphatidylglycerol phosphate phosphatase (n-C18:1) H2O[c] + PGP181[m] ⇒ PG181[m] + PI[c] Peptidyl-tRNA hydrolysis (MET) [c]: AMP_MET + H2O ⇒ AMP + MET Triacylglycerol lipase (octadecanoate) (MG_310) [e]: (3) H2O + TRISTEARIN ⇔ GL + (3) H + (3) OCDCA 5'-nucleotidase (8-Oxo-2'-deoxyguanosine monophosphate; 8-hydroxy-2'-deoxyguanosine monophosphate) [c]: H2O + oxo8dGMP ⇒ oxo8dG + PI 5'-nucleotidase (2'-deoxyinosine monophosphate) [c]: dINSMP + H2O ⇒ dINS + PI Dipeptide import (Gly-Gly) [c]: GlyGly + H2O ⇒ (2) GLY drug efflux (nisin) via ABC system ATP[c] + H2O[c] + nisin[c] ⇒ ADP[c] + H[c] + nisin[e] + PI[c] RNA cleavage (AMP) [c]: ApAp + H2O ⇒ (2) AMP + H tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER Protein degradation (GLN) [c]: ATP + GlnGln + (2) H2O ⇒ ADP + (2) GLN + H + PI Triacylglycerol lipase (tripalmitin) (MG_310) [e]: (3) H2O + TRIPALMITIN ⇔ GL + (3) H + (3) HDCA Peptidyl-tRNA hydrolysis (HIS) [c]: AMP_HIS + H2O ⇒ AMP + HIS 5'-nucleotidase (2'-deoxythymidine monophosphate-[6,4]-2'-deoxycytidine monophosphate dewar product) [c]: dTMP64dCMP_dewar + (2) H2O ⇒ dT64dC_dewar + (2) PI Protein degradation (GLU) [c]: ATP + GluGlu + (2) H2O ⇒ ADP + (2) GLU + H + PI Protein degradation (ILE) [c]: ATP + (2) H2O + IleIle ⇒ ADP + H + (2) ILE + PI peroxidase (cumene hydroperoxide) [c]: (2) CHP + MG_427_DIMER ⇒ (2) DMBA + (2) H2O + MG_427_DIMER_ox spermidine transport via ABC system ATP[c] + H2O[c] + SPMD[e] ⇒ ADP[c] + H[c] + PI[c] + SPMD[c] 5'-nucleotidase (2'-deoxythymidine monophosphate-[6,4]-2'-deoxythymidine monophosphate dewar product) [c]: dTMP64dTMP_dewar + (2) H2O ⇒ dT64dT_dewar + (2) PI peroxidase (tert-butylhydroperoxide) [c]: MG_454_DIMER + (2) tBHP ⇒ (2) H2O + MG_454_DIMER_ox + (2) tBOH base excision (8-hydroxyadenine) [c]: H2O + oxo8dAMP ⇒ DR5P + oxo8AD Protein degradation (PRO) [c]: ATP + (2) H2O + ProPro ⇒ ADP + H + PI + (2) PRO Triacylglycerol lipase (tri_hdcea_in) (MG_327) [e]: (3) H2O + TRI_HDCEA_IN ⇔ GL + (3) H + (3) HDCEA 5'-nucleotidase (2'-deoxycytidine monophosphate-[6,4]-2'-deoxycytidine monophosphate) [c]: dCMP64dCMP + (2) H2O ⇒ dC64dC + (2) PI Peptidyl-tRNA hydrolysis (ASN) [c]: AMP_ASN + H2O ⇒ AMP + ASN 5'-nucleotidase (7-methyl-2'-deoxyguanosine monophosphate) [c]: H2O + m7dGMP ⇒ m7dG + PI Dipeptide import (Asn-Asn) [c]: AsnAsn + H2O ⇒ (2) ASN Chromosome segregation (Era) [c]: GTP + H2O ⇒ GDP + H + PI tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER sugar phosphatase (sorbitol-6-phosphate) [c]: H2O + SBT6P ⇒ H + PI + SBT 5'-nucleotidase (N6-carbamoylmethyl-2'-deoxyadenosine monophosphate) [c]: carbamoylmethyl6dAMP + H2O ⇒ carbamoylmethyl6dA + PI tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER DNA internalization (dTpdTp) (2) dTpdTp[e] + H2O[e] ⇒ (2) DTMP[e] + dTpdTp[c] + H[e] Peptidyl-tRNA hydrolysis (ASP) [c]: AMP_ASP + H2O ⇒ AMP + ASP RNA cleavage (GMP) [c]: GpGp + GTP + (2) H2O ⇒ GDP + (2) GMP + (2) H + PI deoxyribonuclease (dTMP) [c]: dTpdTp + H2O ⇒ (2) DTMP + H phosphatidylglycerol phosphate phosphatase (n-C16:1) H2O[c] + PGP161[m] ⇒ PG161[m] + PI[c] deoxyribose 5'-phosphate lyase [c]: dRibose5P_dRibose5P + H2O ⇒ (2) DR5P + H tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER Triacylglycerol lipase (dibutyrin) (MG_344) [e]: DIBUTYRIN + (2) H2O ⇔ (2) BUT + GL Triacylglycerol lipase (triolein) (MG_327) [e]: (3) H2O + TRIOLEIN ⇔ GL + (3) H + (3) OCDCEA Glycerophosphodiester phosphodiesterase (Glycerophosphoinositol) [c]: GPI + H2O ⇒ GL3P + INOS DNA base amination (5-hydroxymethyluracil ==> a-glutamylthymine; L-glutamate) [c]: GLU + H + hm5URA ⇒ gluTHY + H2O RNA cleavage (UMP) [c]: H2O + UpUp ⇒ H + (2) UMP 5'-nucleotidase (UmMP) [c]: H2O + UmMP ⇒ PI + Um sugar phosphatase (sn-glycerol-1-phosphate) [c]: H2O + SNGLYP ⇒ GL + PI Protein degradation (ARG) [c]: ArgArg + ATP + (2) H2O ⇒ ADP + (2) ARG + H + PI 5'-nucleotidase (dTMP) [c]: DTMP + H2O ⇒ DT + PI oxaloacetate carboxylase [c]: ATP + CO2 + H2O + PYR ⇔ ADP + (2) H + OA + PI Protein polymerization (Ile) [c]: (2) ILE ⇒ H2O + IleIle tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER Triacylglycerol lipase (tri_ttdcea_in) (MG_344) [e]: (3) H2O + TRI_TTDCEA_IN ⇔ GL + H + (3) TTDCEA tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER oligopeptide (Lys) transport via ABC system ATP[c] + H2O[c] + LysLys[e] ⇒ ADP[c] + H[c] + LysLys[c] + PI[c] Protein degradation (MET) [c]: ATP + (2) H2O + MetMet ⇒ ADP + H + (2) MET + PI 5'-nucleotidase (2'-O-ethyl-2'-deoxythymidine monophosphate) [c]: H2O + O2edTMP ⇒ O2edT + PI 5'-nucleotidase (a-glutamyl-2'-deoxythymidine monophosphate) [c]: gludTMP + H2O ⇒ gludT + PI tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER iron (III) transporting ATPase ATP[c] + FE3[e] + H2O[c] ⇒ ADP[c] + FE3[c] + H[c] + PI[c] tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER 2',3'-cyclic-nucleotide phosphodiesterase (CMP) [c]: C23CMP + H2O ⇒ C3MP + H glutamine hydrolysis [c]: GLN + H2O ⇒ GLU + H + NH3 calcium-transporting ATPase ATP[c] + CA[e] + H2O[c] ⇒ ADP[c] + CA[c] + H[c] + PI[c] tRNA U34 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s2UMP + SER 5'-nucleotidase (5-Carbouy-2'-deoxyuridine monophosphate) [c]: c5dUMP + H2O ⇒ c5dU + PI RNA cleavage (GMP) [c]: GpGp + H2O ⇒ (2) GMP + H 5'-nucleotidase (2'-O-methyl-2'-deoxycytidine monophosphate) [c]: H2O + O2mdCMP ⇒ O2mdC + PI base excision (uracil) [c]: DUMP + H2O ⇒ DR5P + URA Spontaneous base loss (guanine) [c]: DGMP + H2O ⇒ DR5P + GN DNA recombination Holliday junction resolution [c]: (2) dRibose5P_dRibose5P + (2) H2O ⇒ (4) DR5P + (2) H Peptidyl-tRNA hydrolysis (GLN) [c]: AMP_GLN + H2O ⇒ AMP + GLN base excision (4,6-diamino-5-N-formamidopyrimidine) [c]: FAPydAMP + H2O ⇒ DR5P + FAPyAD tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER adenosylhomocysteinase [c]: AHCYS + H2O ⇒ ADN + HCYS ribonucleoside-diphosphate reductase maintenance [c]: (2) FMNH2 + (2) MG_124_MONOMER + MG_229_231_TETRAMER_ox ⇒ (2) FMN + (2) H2O + (2) MG_124_MONOMER_ox + MG_229_231_TETRAMER deoxyribonuclease (dGMP) [c]: dGpdGp + H2O ⇒ (2) DGMP + H 5'-nucleotidase (1-ethyl-2'-deoxyadenosine monophosphate) [c]: e1dAMP + H2O ⇒ e1dA + PI DNA cleavage (dGMP) [c]: dGpdGp + H2O ⇒ (2) DGMP + H DnaA inactivation [c]: H2O + MG_469_1MER_ATP ⇒ H + MG_469_1MER_ADP + PI 5'-nucleotidase (cyclobutane 2'-deoxythymidine monophosphate dimer) [c]: cyclobutane_dTMPdTMP + (2) H2O ⇒ cyclobutane_dTdT + (2) PI Triacylglycerol lipase (Tween 80) (MG_327) [e]: H2O + TWEEN80 ⇔ H + OCDCEA + POE_SORBITAN 5'-nucleotidase (3'-hydroxymethyl-2'-deoxycytidine monophosphate) [c]: H2O + hm3dCMP ⇒ hm3dC + PI Spontaneous base loss (cytosine) [c]: DCMP + H2O ⇒ CSN + DR5P 5'-nucleotidase (5-hydroxy-6-hydroxy-2'-deoxythymidine monophosphate) [c]: H2O + ho5ho6dTMP ⇒ ho5ho6dT + PI DNA base glucosyl transfer (5-hydroxymethyluracil ==> beta-D-glucosylhydroxymethyluracil; base J; glucose) [c]: GLC + hm5URA ⇒ glchm5URA + H2O adenine deaminase [c]: AD + H2O ⇒ HYXN + NH3 iron (II) transporting ATPase ATP[c] + FE2[e] + H2O[c] ⇒ ADP[c] + FE2[c] + H[c] + PI[c] 5'-nucleotidase (5-hydroxy-6-hydroxy-2'-deoxyuridine monophosphate) [c]: H2O + ho5ho6dUMP ⇒ ho5ho6dU + PI 5'-nucleotidase (3-ethyl-2'-deoxyguanosine monophosphate) [c]: e3dGMP + H2O ⇒ e3dG + PI Spontaneous base loss (adenine) [c]: DAMP + H2O ⇒ AD + DR5P Protein polymerization (Gln) [c]: (2) GLN ⇒ GlnGln + H2O oligopeptide (Cys) transport via ABC system ATP[c] + CysCys[e] + H2O[c] ⇒ ADP[c] + CysCys[c] + H[c] + PI[c] Spontaneous base deamination (guanine ==> xanthine) [c]: GN + H2O ⇒ NH3 + XAN Protein translocation (SRP) [c]: GTP + H2O ⇒ GDP + H + PI 5'-nucleotidase (1-methyl-2'-deoxyguanosine monophosphate) [c]: H2O + m1dGMP ⇒ m1dG + PI 5'-nucleotidase (6'-O-methyl-2'-deoxyguanosine monophosphate) [c]: H2O + O6mdGMP ⇒ O6mdG + PI cobalt transport via ABC system ATP[c] + CO[e] + H2O[c] ⇒ ADP[c] + CO[c] + H[c] + PI[c] 5'-nucleotidase (5-hydroxymethyl-2'-deoxyuridine monophosphate) [c]: H2O + hm5dUMP ⇒ hm5dU + PI drug efflux (doxorubicin) via ABC system ATP[c] + doxorubicin[c] + H2O[c] ⇒ ADP[c] + doxorubicin[e] + H[c] + PI[c] Protein degradation (ILE) [c]: ATP + (2) H2O + IleIle ⇒ ADP + H + (2) ILE + PI Protein polymerization (Met) [c]: (2) MET ⇒ H2O + MetMet 5'-nucleotidase (8,5'-cyclo-2'-deoxyadenosine monophosphate) [c]: cyclodAMP + H2O ⇒ cyclodA + PI 5'-nucleotidase (m6AMP) [c]: H2O + m6AMP ⇒ m6A + PI tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER Dipeptide import (Thr-Thr) [c]: H2O + ThrThr ⇒ (2) THR DNA resection (dGMP) [c]: dGpdGp + H2O ⇒ (2) DGMP + H Triacylglycerol lipase (trilaurin) (MG_310) [e]: (3) H2O + TRILAURIN ⇔ (3) DDCA + GL + (3) H 5'-nucleotidase (1-ethyl-2'-deoxyguanosine monophosphate) [c]: e1dGMP + H2O ⇒ e1dG + PI Protein degradation (MET) [c]: ATP + (2) H2O + MetMet ⇒ ADP + H + (2) MET + PI tRNA uridine 5-carboxymethylaminomethyl modification enzyme [c]: FTHF5 + GLY + s2UMP ⇒ cmnm5s2UMP + H2O + THF 5'-nucleotidase (1,N6-etheno-2'-deoxyadenosine monophosphate) [c]: epsilon1N6dAMP + H2O ⇒ epsilon1N6dA + PI tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER Dipeptide import (Gln-Gln) [c]: GlnGln + H2O ⇒ (2) GLN cytidine deaminase [c]: CYTD + H2O ⇒ NH3 + URI tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER Triacylglycerol lipase (triolein) (MG_344) [e]: (3) H2O + TRIOLEIN ⇔ GL + (3) H + (3) OCDCEA glutamyl-tRNA (MG502) amidotransferase [c]: ATP + GLN + GLU + H2O ⇒ ADP + GLN + GLU + H + PI RNA cleavage (CMP) [c]: CpCp + H2O ⇒ (2) CMP + H 5'-nucleotidase (5-formyl-2'-deoxyuridine monophosphate) [c]: f5dUMP + H2O ⇒ f5dU + PI purine nucleoside phosphoramidase AMP-NH2 [e]: AMP_NH2 + H2O ⇒ AMP + (2) H + NH3 Protein degradation (LYS) [c]: ATP + (2) H2O + LysLys ⇒ ADP + H + (2) LYS + PI Triacylglycerol lipase (tributyrin) (MG_327) [e]: (3) H2O + TRIBUTYRIN ⇔ (3) BUT + GL Glycerophosphodiester phosphodiesterase (Glycerophosphoinositol) [c]: GPI + H2O ⇒ GL3P + INOS Protein degradation (LEU) [c]: ATP + (2) H2O + LeuLeu ⇒ ADP + H + (2) LEU + PI 2',3'-cyclic-nucleotide phosphodiesterase (GMP) [c]: G23CMP + H2O ⇒ G3MP + H Peptidyl-tRNA hydrolysis (TYR) [c]: AMP_TYR + H2O ⇒ AMP + TYR peroxidase (tert-butylhydroperoxide) [c]: MG_427_DIMER + (2) tBHP ⇒ (2) H2O + MG_427_DIMER_ox + (2) tBOH 5'-nucleotidase (cyclobutane 2'-deoxythymidine monophosphate 2'-deoxycytidine monophosphate) [c]: cyclobutane_dTMPdCMP + (2) H2O ⇒ cyclobutane_dTdC + (2) PI 5'-nucleotidase (5-hydroxy-2'-deoxyuridine monophosphate) [c]: H2O + ho5dUMP ⇒ ho5dU + PI 5'-nucleotidase (dCMP) [c]: DCMP + H2O ⇒ DC + PI Protein polymerization (Pro) [c]: (2) PRO ⇒ H2O + ProPro Protein polymerization (Cys) [c]: (2) CYS ⇒ CysCys + H2O Protein degradation (ASN) [c]: AsnAsn + ATP + (2) H2O ⇒ ADP + (2) ASN + H + PI 5'-nucleotidase (beta-D-glucosylhydroxymethyl-2'-deoxyuridine monophosphate) [c]: glchm5dUMP + H2O ⇒ glchm5dU + PI 5'-nucleotidase (5'-dihydroxypentyl-2'-deoxyuridine monophosphate) [c]: dhpdUMP + H2O ⇒ dhpdU + PI Protein degradation (SER) [c]: ATP + (2) H2O + SerSer ⇒ ADP + H + PI + (2) SER Triacylglycerol lipase (tripalmitin) (MG_310) [e]: (3) H2O + TRIPALMITIN ⇔ GL + (3) H + (3) HDCA Protein degradation (LYS) [c]: ATP + (2) H2O + LysLys ⇒ ADP + H + (2) LYS + PI Triacylglycerol lipase (tributyrin) (MG_310) [e]: (3) H2O + TRIBUTYRIN ⇔ (3) BUT + GL Phosphotyrosine phosphatase [c]: H2O + pTYR ⇒ PI + TYR 5'-nucleotidase (8,5'-cyclo-2'-deoxyguanosine monophosphate) [c]: cyclodGMP + H2O ⇒ cyclodG + PI peroxidase (hydrogen peroxide) [c]: (2) H2O2 + MG_427_DIMER ⇒ (4) H2O + MG_427_DIMER_ox Protein polymerization (Val) [c]: (2) VAL ⇒ H2O + ValVal 5'-nucleotidase (3-methyl-2'-deoxycytidine monophosphate) [c]: H2O + m3dCMP ⇒ m3dC + PI 5'-nucleotidase (m62AMP) [c]: H2O + m62AMP ⇒ m62A + PI Protein polymerization (Ala) [c]: (2) ALA ⇒ AlaAla + H2O Protein polymerization (Arg) [c]: (2) ARG ⇒ ArgArg + H2O tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER 5'-nucleotidase (m2GMP) [c]: H2O + m2GMP ⇒ m2G + PI Phosphonoacetaldehyde hydrolase [c]: H2O + PPAA ⇒ ACAL + (2) H + PI Protein degradation (TYR) [c]: ATP + (2) H2O + TyrTyr ⇒ ADP + H + PI + (2) TYR Triacylglycerol lipase (dibutyrin) (MG_310) [e]: DIBUTYRIN + (2) H2O ⇔ (2) BUT + GL Protein polymerization (Lys) [c]: (2) LYS ⇒ H2O + LysLys Peptidyl-tRNA hydrolysis (ARG) [c]: AMP_ARG + H2O ⇒ AMP + ARG + H tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER Glycerophosphodiester phosphodiesterase (Glycerophosphoglycerol) [c]: GPG + H2O ⇒ GL + GL3P sugar phosphatase (ribose-5-phosphate) [c]: H2O + R5P ⇒ PI + RIB tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER RNA cleavage (GMP) [c]: GpGp + H2O ⇒ (2) GMP + H Triacylglycerol lipase (octadecanoate) (MG_344) [e]: (3) H2O + TRISTEARIN ⇔ GL + (3) H + (3) OCDCA 5'-nucleotidase (8-aza-2'-deoxyadenosine monophosphate) [c]: aza8dAMP + H2O ⇒ aza8dA + PI Peptidyl-tRNA hydrolysis (GLU) [c]: AMP_GLU + H2O ⇒ AMP + GLU + H Protein polymerization (Ser) [c]: (2) SER ⇒ H2O + SerSer Magnesium-transporting ATPase ATP[c] + H2O[c] + MG[e] ⇒ ADP[c] + H[c] + MG[c] + PI[c] Dipeptide import (Ser-Ser) [c]: H2O + SerSer ⇒ (2) SER hydroxyl radical generation gamma_radiation[e] + H2O[c] ⇒ hydride[c] + hydroxyl_radical[c] 5'-nucleotidase (3-methyl-2'-deoxyguanosine monophosphate) [c]: H2O + m3dGMP ⇒ m3dG + PI 5'-nucleotidase (2'-deoxythymidine monophosphate-[6,4]-2'-deoxythymidine monophosphate) [c]: dTMP64dTMP + (2) H2O ⇒ dT64dT + (2) PI tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER Spontaneous abasic site strand break [c]: dRibose5P_dRibose5P + H2O ⇒ (2) DR5P + H nicotinic acid mononucleotide pyrophosphorylase [c]: ATP + (3) H + H2O + NAC + PRPP ⇒ ADP + NAMN + PI + PPI tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER 5'-nucleotidase (m7GMP) [c]: H2O + m7GMP ⇒ m7G + PI purine nucleoside phosphoramidase AMP-morpholidate [e]: AMP_Mor + H2O ⇒ AMP + (2) H + MOR 5'-nucleotidase (s4UMP) [c]: H2O + s4UMP ⇒ PI + s4U FtsZ ring contraction [c]: (18) H2O + (2) MG_224_9MER_GTP ⇒ (18) H + (2) MG_224_9MER_GDP + (18) PI DNA recombination strand exchange [c]: ATP + H2O ⇒ ADP + H + PI nickel transport via ABC system ATP[c] + H2O[c] + NI[e] ⇒ ADP[c] + H[c] + NI[c] + PI[c] 5'-apurinic/apyrimidinic endonuclease [c]: dRibose5P_dRibose5P + H2O ⇒ (2) DR5P + H oligopeptide (Leu) transport via ABC system ATP[c] + H2O[c] + LeuLeu[e] ⇒ ADP[c] + H[c] + LeuLeu[c] + PI[c] Triacylglycerol lipase (Tween 80) (MG_310) [e]: H2O + TWEEN80 ⇔ H + OCDCEA + POE_SORBITAN Deformylation [c]: fMetMet + H2O ⇒ FOR + H + MetMet Glycerophosphodiester phosphodiesterase (Glycerophosphoethanolamine) [c]: GPE + H2O ⇒ ETN + GL3P + H tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER RNA cleavage (AMP) [c]: ApAp + H2O ⇒ (2) AMP + H peroxidase (hydrogen peroxide) [c]: (2) H2O2 + MG_454_DIMER ⇒ (4) H2O + MG_454_DIMER_ox Spontaneous base deamination (adenine ==> hypoxanthine) [c]: AD + H2O ⇒ HYXN + NH3 tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER Dipeptide import (Ala-Ala) [c]: AlaAla + H2O ⇒ (2) ALA Protein degradation (GLU) [c]: ATP + GluGlu + (2) H2O ⇒ ADP + (2) GLU + H + PI 5'-nucleotidase (5-Hydroxy-2'-deoxycytidine monophosphate) [c]: H2O + ho5dCMP ⇒ ho5dC + PI Peptidyl-tRNA hydrolysis (CYS) [c]: AMP_CYS + H2O ⇒ AMP + CYS Protein degradation (TRP) [c]: ATP + (2) H2O + TrpTrp ⇒ ADP + H + PI + (2) TRP 5'-nucleotidase (s2UMP) [c]: H2O + s2UMP ⇒ PI + s2U Dipeptide import (Asp-Asp) [c]: AspAsp + H2O ⇒ (2) ASP 5'-deoxyribophosphodiesterase; exonuclease [c]: dRibose5P_dRibose5P + H2O ⇒ (2) DR5P + H 5'-nucleotidase (3-ethyl-2'-deoxyadenosine monophosphate) [c]: e3dAMP + H2O ⇒ e3dA + PI RNA cleavage (CMP) [c]: CpCp + GTP + (2) H2O ⇒ (2) CMP + GDP + (2) H + PI Protein degradation (SER) [c]: ATP + (2) H2O + SerSer ⇒ ADP + H + PI + (2) SER Protein polymerization (Asn) [c]: (2) ASN ⇒ AsnAsn + H2O 5'-nucleotidase (3,N4-etheno-2'-deoxycytidine monophosphate) [c]: epsilon3N4dCMP + H2O ⇒ epsilon3N4dC + PI Protein polymerization (Trp) [c]: (2) TRP ⇒ H2O + TrpTrp tRNA uridine 5-carboxymethylaminomethyl modification enzyme [c]: FTHF5 + GLY + s2UMP ⇒ cmnm5s2UMP + H2O + THF Glycerophosphodiester phosphodiesterase (Glycerophosphocholine) [c]: GPCH + H2O ⇒ CHOL + GL3P Protein polymerization (Phe) [c]: (2) PHE ⇒ H2O + PhePhe tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER DNA internalization (dApdAp) (2) dApdAp[e] + H2O[e] ⇒ (2) DAMP[e] + dApdAp[c] + H[e] tRNA uridine 5-carboxymethylaminomethyl modification enzyme [c]: FTHF5 + GLY + s2UMP ⇒ cmnm5s2UMP + H2O + THF 5'-nucleotidase (3'-ethyl-2'-deoxythymidine monophosphate) [c]: e3dTMP + H2O ⇒ e3dT + PI DNA cleavage (dCMP) [c]: dCpdCp + H2O ⇒ (2) DCMP + H EcoD DNA restriction/modification, DNA restriction [c]: ATP + (2) dRibose5P_dRibose5P + (3) H2O ⇒ ADP + (4) DR5P + (3) H + PI phosphoserine phosphatase [c]: H2O + pSER ⇒ PI + SER Protein degradation (GLY) [c]: ATP + GlyGly + (2) H2O ⇒ ADP + (2) GLY + H + PI formylmethionine hydrolysis [c]: FMET + H2O ⇔ FOR + H + MET 5'-nucleotidase (4'-O-methyl-2'-deoxythymidine monophosphate) [c]: H2O + O4mdTMP ⇒ O4mdT + PI L-methionine-S-sulfoxide reductase [c]: H2O + MET + MG_124_MONOMER_ox ⇔ METSOXSL + MG_124_MONOMER Peptidyl-tRNA hydrolysis (MET) [c]: AMP_FMET + H2O ⇒ AMP + FMET sugar phosphatase (sn-glycerol-2-phosphate) [c]: GL2P + H2O ⇒ GL + PI Ribosome assembly (EngB) [c]: GTP + H2O ⇒ GDP + H + PI 5'-nucleotidase (N6-(2-Deoxy-α,β-d-erythro-pentofuranosyl)-2,6-diamino-4-hydroxy-5-formamidopyrimidine monophosphate) [c]: FAPydGMP + H2O ⇒ FAPydG + PI 5'-nucleotidase (2'-O-methyl-2'-deoxythymidine monophosphate) [c]: H2O + O2mdTMP ⇒ O2mdT + PI Protein degradation (ASN) [c]: AsnAsn + ATP + (2) H2O ⇒ ADP + (2) ASN + H + PI purine nucleoside phosphoramidase GMP-Lysine [e]: GMP_Lysine + H2O ⇒ GMP + H + LYS oligopeptide (Ser) transport via ABC system ATP[c] + H2O[c] + SerSer[e] ⇒ ADP[c] + H[c] + PI[c] + SerSer[c] ribonucleoside-diphosphate reductase (CDP) [c]: (2) CDP + MG_229_231_TETRAMER + O2 ⇒ (2) DCDP + (2) H2O + MG_229_231_TETRAMER_ox 5'-nucleotidase (2'-deoxycytidine monophosphate-[6,4]-2'-deoxythymidine monophosphate) [c]: dCMP64dTMP + (2) H2O ⇒ dC64dT + (2) PI DNA internalization (dGpdGp) (2) dGpdGp[e] + H2O[e] ⇒ (2) DGMP[e] + dGpdGp[c] + H[e] tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER RNA cleavage (GMP) [c]: ATP + GpGp + (2) H2O ⇒ ADP + (2) GMP + (2) H + PI 5'-nucleotidase (2-'deoxycytidine monophosphate glycol) [c]: dCMP_GLYC + H2O ⇒ dC_GLYC + PI Dipeptide import (Trp-Trp) [c]: H2O + TrpTrp ⇒ (2) TRP 5'-nucleotidase (AMP) [c]: AMP + H2O ⇒ ADN + PI 5'-nucleotidase (4-ethyl-2'-deoxycytidine monophosphate) [c]: e4dCMP + H2O ⇒ e4dC + PI 5'-nucleotidase (m1GMP) [c]: H2O + m1GMP ⇒ m1G + PI Peptidyl-tRNA hydrolysis (PHE) [c]: AMP_PHE + H2O ⇒ AMP + PHE 5'-nucleotidase (2'-deoxy-3'-(5-hydroxy-5-methyl-hydantoin)-ribose monophosphate) [c]: H2O + hm5hydantoin_dRiboseMP ⇒ hm5hydantoin_dRibose + PI 5'-nucleotidase (IMP) [c]: H2O + IMP ⇒ INS + PI tRNA U34 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s2UMP + SER oligopeptide (Ala) transport via ABC system AlaAla[e] + ATP[c] + H2O[c] ⇒ ADP[c] + AlaAla[c] + H[c] + PI[c] glycosyltransferase (Phosphatidylglycerol (dioctadec-11-enoyl, n-C18:1) ==> Diacylglycerol (n-18:1)) H2O[c] + PG181[m] ⇒ DAG181[m] + GL3P[c] Triacylglycerol lipase (trilaurin) (MG_344) [e]: (3) H2O + TRILAURIN ⇔ (3) DDCA + GL + (3) H methenyltetrahydrofolate cyclohydrolase [c]: H2O + METHF ⇔ FTHF10 + H 5'-nucleotidase (1,N2-etheno-2'-deoxyguanosine monophosphate) [c]: epsilon1N2dGMP + H2O ⇒ epsilon1N2dG + PI DNA cleavage (dTMP) [c]: dTpdTp + H2O ⇒ (2) DTMP + H Triacylglycerol lipase (methyl_ocdcea) (MG_310) [e]: H2O + METHYL_OCDCEA ⇔ CH3OH + H + OCDCEA DNA recombination Holliday junction migration [c]: ATP + H2O ⇒ ADP + H + PI Protein polymerization (Gly) [c]: (2) GLY ⇒ GlyGly + H2O Negative supercoiling (gyrase) [c]: (2) ATP + (2) H2O ⇒ (2) ADP + (2) H + (2) PI RNA cleavage (UMP) [c]: H2O + UpUp ⇒ H + (2) UMP 3'-apurinic/apyrimidinic lyase [c]: dRibose5P_dRibose5P + H2O ⇒ (2) DR5P + H 5'-nucleotidase (8-methyl-2'-deoxyguanosine monophosphate) [c]: H2O + m8dGMP ⇒ m8dG + PI deoxycytidine deaminase [c]: DC + H2O ⇒ DU + NH3 5'-nucleotidase (2'-deoxyxanthosine monophosphate) [c]: dXANMP + H2O ⇒ dXAN + PI Protein degradation (TYR) [c]: ATP + (2) H2O + TyrTyr ⇒ ADP + H + PI + (2) TYR 5'-nucleotidase (3-methyl-2'-deoxyadenosine monophosphate) [c]: H2O + m3dAMP ⇒ m3dA + PI phosphate transport via ABC system ATP[c] + H2O[c] + PI[e] ⇒ ADP[c] + H[c] + (2) PI[c] tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER DNA resection (dTMP) [c]: dTpdTp + H2O ⇒ (2) DTMP + H 5'-nucleotidase (2'-deoxythymidine monophosphate glycol) [c]: dTMP_GLYC + H2O ⇒ dT_GLYC + PI Triacylglycerol lipase (trimyristin) (MG_344) [e]: (3) H2O + TRIMYRISTIN ⇔ GL + (3) H + (3) TTDCA 5'-nucleotidase (cyclobutane 2'-deoxycytidine monophosphate dimer) [c]: cyclobutane_dCMPdCMP + (2) H2O ⇒ cyclobutane_dCdC + (2) PI Protein degradation (PRO) [c]: ATP + (2) H2O + ProPro ⇒ ADP + H + PI + (2) PRO 5'-nucleotidase (beta-D-glucosylhydroxymethyl-2'-deoxycytidine monophosphate) [c]: glchm5dCMP + H2O ⇒ glchm5dC + PI tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER Dipeptide import (Phe-Phe) [c]: H2O + PhePhe ⇒ (2) PHE tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER enolase [c]: G2P ⇔ H2O + PEP ribonucleoside-diphosphate reductase (UDP) [c]: MG_229_231_TETRAMER + O2 + (2) UDP ⇒ (2) DUDP + (2) H2O + MG_229_231_TETRAMER_ox zinc-transporting ATPase ATP[c] + H2O[c] + ZN[e] ⇒ ADP[c] + H[c] + PI[c] + ZN[c] RNA cleavage (UMP) [c]: H2O + UpUp ⇒ H + (2) UMP Dipeptide import (Lys-Lys) [c]: H2O + LysLys ⇒ (2) LYS Triacylglycerol lipase (tri_ttdcea_in) (MG_327) [e]: (3) H2O + TRI_TTDCEA_IN ⇔ GL + H + (3) TTDCEA Glycerophosphodiester phosphodiesterase (Glycerophosphoethanolamine) [c]: GPE + H2O ⇒ ETN + GL3P + H sugar phosphatase (mannose-6-phosphate) [c]: H2O + MAN6P ⇒ MAN + PI oligopeptide (Glu) transport via ABC system ATP[c] + GluGlu[e] + H2O[c] ⇒ ADP[c] + GluGlu[c] + H[c] + PI[c] DNA resection (dAMP) [c]: dApdAp + H2O ⇒ (2) DAMP + H Protein degradation (CYS) [c]: ATP + CysCys + (2) H2O ⇒ ADP + (2) CYS + H + PI phosphatidylglycerol phosphate phosphatase (n-C16:0) H2O[c] + PGP160[m] ⇒ PG160[m] + PI[c] glycosyltransferase (Phosphatidylglycerol (dihexadecanoyl, n-C16:0) ==> Diacylglycerol (n-16:0)) H2O[c] + PG160[m] ⇒ DAG160[m] + GL3P[c] + (2) H[c] RNA cleavage (CMP) [c]: ATP + CpCp + (2) H2O ⇒ ADP + (2) CMP + (2) H + PI Spontaneous base loss (thymine) [c]: DTMP + H2O ⇒ DR5P + THY purine nucleoside phosphoramidase GMP-morpholidate [e]: GMP_Mor + H2O ⇒ GMP + (2) H + MOR 5'-nucleotidase (8-hydroxy-2'-deoxyadenosine monophosphate) [c]: H2O + oxo8dAMP ⇒ oxo8dA + PI Protein degradation (ASP) [c]: AspAsp + ATP + (2) H2O ⇒ ADP + (2) ASP + H + PI Triacylglycerol lipase (Tween 80) (MG_344) [e]: H2O + TWEEN80 ⇔ H + OCDCEA + POE_SORBITAN oligopeptide (Ile) transport via ABC system ATP[c] + H2O[c] + IleIle[e] ⇒ ADP[c] + H[c] + IleIle[c] + PI[c] Peptidyl-tRNA hydrolysis (ALA) [c]: AMP_ALA + H2O ⇒ ALA + AMP DNA excision [c]: ATP + H2O ⇒ ADP + H + PI tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER deoxyribonuclease (dCMP) [c]: dCpdCp + H2O ⇒ (2) DCMP + H 5'-nucleotidase (8-ethyl-2'-deoxyguanosine monophosphate) [c]: e8dGMP + H2O ⇒ e8dG + PI RNA cleavage (AMP) [c]: ApAp + GTP + (2) H2O ⇒ (2) AMP + GDP + (2) H + PI DNA radiation (gamma-ray) induced base oxidation (5-hydroxymethyluracil ==> 5-formyluracil; hydroxyl radical) gamma_radiation[e] + hm5URA[c] + hydroxyl_radical[c] ⇒ f5URA[c] + H[c] + H2O[c] 5'-nucleotidase (2-amino-2'-deoxyadenosine monophosphate) [c]: H2O + n2dAMP ⇒ n2dA + PI Protein translocation (SecA) [c]: ATP + H2O ⇒ ADP + H + PI Protein degradation (GLY) [c]: ATP + GlyGly + (2) H2O ⇒ ADP + (2) GLY + H + PI ATP synthase (four protons for one ATP) (periplasm) ADP[c] + (4) H[e] + H[c] + PI[c] ⇔ ATP[c] + (4) H[c] + H2O[c] Dipeptide import (Leu-Leu) [c]: H2O + LeuLeu ⇒ (2) LEU Protein degradation (VAL) [c]: ATP + (2) H2O + ValVal ⇒ ADP + H + PI + (2) VAL 5'-nucleotidase (cyclobutane 2'-deoxycytidine monophosphate 2'-deoxythymidine monophosphate) [c]: cyclobutane_dCMPdTMP + (2) H2O ⇒ cyclobutane_dCdT + (2) PI molybdate transporting ATPase ATP[c] + H2O[c] + MOBD[e] ⇒ ADP[c] + H[c] + MOBD[c] + PI[c] oligopeptide (Asp) transport via ABC system AspAsp[e] + ATP[c] + H2O[c] ⇒ ADP[c] + AspAsp[c] + H[c] + PI[c] Peptidyl-tRNA hydrolysis (PRO) [c]: AMP_PRO + H2O ⇒ AMP + PRO 5'-nucleotidase (k2CMP) [c]: H2O + k2CMP ⇒ k2C + PI Triacylglycerol lipase (tri_hdcea_in) (MG_344) [e]: (3) H2O + TRI_HDCEA_IN ⇔ GL + (3) H + (3) HDCEA oligopeptide (Gln) transport via ABC system ATP[c] + GlnGln[e] + H2O[c] ⇒ ADP[c] + GlnGln[c] + H[c] + PI[c] 5'-nucleotidase (2'-deoxy-3'-(5-hydroxyhydantoin)-ribose monophosphate) [c]: H2O + ho5hydantoin_dRiboseMP ⇒ ho5hydantoin_dRibose + PI RNA cleavage (UMP) [c]: GTP + (2) H2O + UpUp ⇒ GDP + (2) H + PI + (2) UMP sugar phosphatase (fructose-6-phosphate) [c]: F6P + H2O ⇒ FRU + PI tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER DNA base alkylation (5-hydroxymethyluracil ==> 5'-dihydroxypentyluracil; 1,2-Butylene glycol) [c]: butylene_glycol + hm5URA ⇒ dhpURA + H2O Protein degradation (LEU) [c]: ATP + (2) H2O + LeuLeu ⇒ ADP + H + (2) LEU + PI Protein degradation (HIS) [c]: ATP + (2) H2O + HisHis ⇒ ADP + H + (2) HIS + PI vinyl chloride activation [c]: H2O + vinyl_chloride ⇒ chloroethylene_oxide + (2) H 5'-nucleotidase (cmnm5s2UMP) [c]: cmnm5s2UMP + H2O ⇒ cmnm5s2U + PI RNA cleavage (GMP) [c]: GpGp + H2O ⇒ (2) GMP + H Triacylglycerol lipase (1-monobutyrin) (MG_344) [e]: H2O + MONOBUTYRIN ⇔ BUT + GL peroxidase (cumene hydroperoxide) [c]: (2) CHP + MG_454_DIMER ⇒ (2) DMBA + (2) H2O + MG_454_DIMER_ox tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER Ribosome assembly (RbgA) [c]: GTP + H2O ⇒ GDP + H + PI inorganic diphosphatase [c]: H2O + PPI ⇒ H + (2) PI Triacylglycerol lipase (triolein) (MG_310) [e]: (3) H2O + TRIOLEIN ⇔ GL + (3) H + (3) OCDCEA tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER sugar phosphatase (2-deoxy-glucose-6-phosphate) [c]: deoxyG6P + H2O ⇒ deoxyGLC + (2) H + PI Protein degradation (PHE) [c]: ATP + (2) H2O + PhePhe ⇒ ADP + H + (2) PHE + PI sugar phosphatase (glucose-6-phosphate) [c]: G6P + H2O ⇒ GLC + PI Chromosome condensation [c]: ATP + H2O + MG_213_214_298_6MER ⇒ H + MG_213_214_298_6MER_ADP + PI 5'-nucleotidase (3-ethyl-2'-deoxycytidine monophosphate) [c]: e3dCMP + H2O ⇒ e3dC + PI Triacylglycerol lipase (methyl_ocdcea) (MG_344) [e]: H2O + METHYL_OCDCEA ⇔ CH3OH + H + OCDCEA RNA cleavage (AMP) [c]: ApAp + ATP + (2) H2O ⇒ ADP + (2) AMP + (2) H + PI Triacylglycerol lipase (tributyrin) (MG_344) [e]: (3) H2O + TRIBUTYRIN ⇔ (3) BUT + GL RNA cleavage (AMP) [c]: ApAp + H2O ⇒ (2) AMP + H Triacylglycerol lipase (trimyristin) (MG_327) [e]: (3) H2O + TRIMYRISTIN ⇔ GL + (3) H + (3) TTDCA Triacylglycerol lipase (tripalmitin) (MG_344) [e]: (3) H2O + TRIPALMITIN ⇔ GL + (3) H + (3) HDCA Dipeptide import (Arg-Arg) [c]: ArgArg + H2O ⇒ (2) ARG 5'-nucleotidase (GMP) [c]: GMP + H2O ⇒ GSN + PI oligopeptide (Asn) transport via ABC system AsnAsn[e] + ATP[c] + H2O[c] ⇒ ADP[c] + AsnAsn[c] + H[c] + PI[c] 5'-nucleotidase (5-methyl-2'-deoxycytidine monophosphate) [c]: H2O + m5dCMP ⇒ m5dC + PI deoxyribonuclease (dAMP) [c]: dApdAp + H2O ⇒ (2) DAMP + H Triacylglycerol lipase (trilaurin) (MG_327) [e]: (3) H2O + TRILAURIN ⇔ (3) DDCA + GL + (3) H oligopeptide (Tyr) transport via ABC system ATP[c] + H2O[c] + TyrTyr[e] ⇒ ADP[c] + H[c] + PI[c] + TyrTyr[c] Glycerophosphodiester phosphodiesterase (Glycerophosphoserine) [c]: GPS + H2O ⇒ GL3P + SER Triacylglycerol lipase (octadecanoate) (MG_327) [e]: (3) H2O + TRISTEARIN ⇔ GL + (3) H + (3) OCDCA tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER Ribosome assembly (EngA) [c]: GTP + H2O ⇒ GDP + H + PI tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER 5'-nucleotidase (GmMP) [c]: GmMP + H2O ⇒ Gm + PI oligopeptide (Phe) transport via ABC system ATP[c] + H2O[c] + PhePhe[e] ⇒ ADP[c] + H[c] + PhePhe[c] + PI[c] glycine hydroxymethyltransferase, reversible [c]: (2) H + SER + THF ⇒ GLY + H2O + METTHF Peptidyl-tRNA hydrolysis (LYS) [c]: AMP_LYS + H2O ⇒ AMP + LYS 5'-nucleotidase (7-ethyl-2'-deoxyadenosine monophosphate) [c]: e7dAMP + H2O ⇒ e7dA + PI purine nucleoside phosphoramidase AMP-Lysine [e]: AMP_Lysine + H2O ⇒ AMP + H + LYS Triacylglycerol lipase (trimyristin) (MG_310) [e]: (3) H2O + TRIMYRISTIN ⇔ GL + (3) H + (3) TTDCA Protein degradation (THR) [c]: ATP + (2) H2O + ThrThr ⇒ ADP + H + PI + (2) THR 5'-nucleotidase (6'-O-ethyl-2'-deoxyguanosine monophosphate) [c]: H2O + O6edGMP ⇒ O6edG + PI D-ribose transport via ABC system ATP[c] + H2O[c] + RIB[e] ⇒ ADP[c] + H[c] + PI[c] + RIB[c] Dipeptide import (Cys-Cys) [c]: CysCys + H2O ⇒ (2) CYS 5'-nucleotidase (5-ethyl-2'-deoxycytidine monophosphate) [c]: e5dCMP + H2O ⇒ e5dC + PI 5'-nucleotidase (dUMP) [c]: DUMP + H2O ⇒ DU + PI tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER 5'-nucleotidase (6-methyl-2'-deoxyadenosine monophosphate) [c]: H2O + m6dAMP ⇒ m6dA + PI DNA unwinding [c]: ATP + H2O ⇒ ADP + H + PI Radiation (gamma-ray) induced strand break dRibose5P_dRibose5P[c] + gamma_radiation[e] + H2O[c] ⇒ (2) DR5P[c] + H[c] Dipeptide import (Glu-Glu) [c]: GluGlu + H2O ⇒ (2) GLU 5'-nucleotidase (N2,3-etheno-2'-deoxyguanosine monophosphate) [c]: epsilonN23dGMP + H2O ⇒ epsilonN23dG + PI Ribosome assembly (Era) [c]: GTP + H2O ⇒ GDP + H + PI tRNA U34 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s2UMP + SER Protein degradation (ARG) [c]: ArgArg + ATP + (2) H2O ⇒ ADP + (2) ARG + H + PI methionine adenosyltransferase [c]: ATP + H2O + MET ⇒ AMET + PI + PPI Protein degradation (VAL) [c]: ATP + (2) H2O + ValVal ⇒ ADP + H + PI + (2) VAL glycerol transport via ABC system ATP[c] + GL3P[e] + H2O[c] ⇒ ADP[c] + GL3P[c] + H[c] + PI[c] Protein degradation (TRP) [c]: ATP + (2) H2O + TrpTrp ⇒ ADP + H + PI + (2) TRP Protein polymerization (Tyr) [c]: (2) TYR ⇒ H2O + TyrTyr DNA incision [c]: ATP + (2) dRibose5P_dRibose5P + (3) H2O ⇒ ADP + (4) DR5P + (3) H + PI RNA cleavage (UMP) [c]: H2O + UpUp ⇒ H + (2) UMP DNA resection (dCMP) [c]: dCpdCp + H2O ⇒ (2) DCMP + H 5'-nucleotidase (7-(2'-oxoethyl)-2'-deoxyguanosine monophosphate) [c]: H2O + oxoethyl7dGMP ⇒ oxoethyl7dG + PI Glycerophosphodiester phosphodiesterase (Glycerophosphoserine) [c]: GPS + H2O ⇒ GL3P + SER RNA cleavage (CMP) [c]: CpCp + H2O ⇒ (2) CMP + H Dipeptide import (Pro-Pro) [c]: H2O + ProPro ⇒ (2) PRO DNA base amination (5-hydroxymethyluracil ==> alpha-putrescinylthymine; putrescine) [c]: hm5URA + PTRC ⇒ (2) H + H2O + putTHY 5'-nucleotidase (N4-(2-Deoxy-alpha,beta-D-erythro-pentofuranosyl)-4,6-diamino-5-formamidopyrimidine monophosphate) [c]: FAPydAMP + H2O ⇒ FAPydA + PI Triacylglycerol lipase (tri_ttdcea_in) (MG_310) [e]: (3) H2O + TRI_TTDCEA_IN ⇔ GL + H + (3) TTDCEA Dipeptide import (Met-Met) [c]: H2O + MetMet ⇒ (2) MET Spontaneous base deamination (5-methylcytosine ==> thymine) [c]: H2O + m5CSN ⇒ NH3 + THY RNA cleavage (UMP) [c]: ATP + (2) H2O + UpUp ⇒ ADP + (2) H + PI + (2) UMP RNA cleavage (CMP) [c]: CpCp + H2O ⇒ (2) CMP + H Protein polymerization (Thr) [c]: (2) THR ⇒ H2O + ThrThr Chromosome segregation (Obg) [c]: GTP + H2O ⇒ GDP + H + PI DNA internalization (dCpdCp) (2) dCpdCp[e] + H2O[e] ⇒ (2) DCMP[e] + dCpdCp[c] + H[e] Dipeptide import (His-His) [c]: H2O + HisHis ⇒ (2) HIS MunI DNA restriction/modification, DNA restriction [c]: (2) dRibose5P_dRibose5P + (2) H2O ⇒ (4) DR5P + (2) H 5'-nucleotidase (5'-hydroxymethyl-2'-deoxycytidine monophosphate) [c]: H2O + hm5dCMP ⇒ hm5dC + PI oligopeptide (Thr) transport via ABC system ATP[c] + H2O[c] + ThrThr[e] ⇒ ADP[c] + H[c] + PI[c] + ThrThr[c] 5'-nucleotidase (4-methyl-2'-deoxycytidine monophosphate) [c]: H2O + m4dCMP ⇒ m4dC + PI Glycerophosphodiester phosphodiesterase (Glycerophosphocholine) [c]: GPCH + H2O ⇒ CHOL + GL3P RNA cleavage (GMP) [c]: GpGp + H2O ⇒ (2) GMP + H Peptidyl-tRNA hydrolysis (TRP) [c]: AMP_TRP + H2O ⇒ AMP + TRP 5'-nucleotidase (alpha-putrescinyl-2'-deoxythymidine monophosphate) [c]: H2O + putdTMP ⇒ PI + putdT 2',3'-cyclic-nucleotide phosphodiesterase (AMP) [c]: A23CMP + H2O ⇒ A3MP + H Triacylglycerol lipase (1-monobutyrin) (MG_310) [e]: H2O + MONOBUTYRIN ⇔ BUT + GL Dipeptide import (Ile-Ile) [c]: H2O + IleIle ⇒ (2) ILE Protein polymerization (Glu) [c]: (2) GLU ⇒ GluGlu + H2O oligopeptide (Arg) transport via ABC system ArgArg[e] + ATP[c] + H2O[c] ⇒ ADP[c] + ArgArg[c] + H[c] + PI[c] 5'-nucleotidase (6-aza-2'-deoxythymidine monophosphate) [c]: aza6dTMP + H2O ⇒ aza6dT + PI phosphothreonine phosphatase [c]: H2O + pTHR ⇒ PI + THR Ribosome assembly (Obg) [c]: GTP + H2O ⇒ GDP + H + PI Protein degradation (HIS) [c]: ATP + (2) H2O + HisHis ⇒ ADP + H + (2) HIS + PI 5'-nucleotidase (5,6-dihydroxy-2'-deoxyuridine monophosphate) [c]: DHdUMP + H2O ⇒ DHdU + PI Glycerophosphodiester phosphodiesterase (Glycerophosphoglycerol) [c]: GPG + H2O ⇒ GL + GL3P Peptidyl-tRNA hydrolysis (VAL) [c]: AMP_VAL + H2O ⇒ AMP + VAL Triacylglycerol lipase (tri_hdcea_in) (MG_310) [e]: (3) H2O + TRI_HDCEA_IN ⇔ GL + (3) H + (3) HDCEA 5'-nucleotidase (m5CMP) [c]: H2O + m5CMP ⇒ m5C + PI 5'-nucleotidase (7-methyl-2'-deoxyadenosine monophosphate) [c]: H2O + m7dAMP ⇒ m7dA + PI tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER 5,10-methenyltetrahydrofolate hydrolysis [c]: H + H2O + METHF ⇒ FTHF5 base excision (8-Oxo-guanine; 8-hydroxyguanine) [c]: H2O + oxo8dGMP ⇒ DR5P + oxo8GN 5'-nucleotidase (6-ethyl-2'-deoxyadenosine monophosphate) [c]: e6dAMP + H2O ⇒ e6dA + PI 5'-nucleotidase (PSIURIMP) [c]: H2O + PSIURIMP ⇒ PI + PSIURI copper-transporting ATPase ATP[c] + CU[e] + H2O[c] ⇒ ADP[c] + CU[c] + H[c] + PI[c] Negative supercoiling (topoisomerase I) [c]: (2) ATP + (2) H2O ⇒ (2) ADP + (2) H + (2) PI Peptidyl-tRNA hydrolysis (THR) [c]: AMP_THR + H2O ⇒ AMP + THR Protein degradation (ASP) [c]: AspAsp + ATP + (2) H2O ⇒ ADP + (2) ASP + H + PI L-glutamine transport via ABC system ATP[c] + GLN[e] + H2O[c] ⇒ ADP[c] + GLN[c] + H[c] + PI[c] base excision (2,6-diamino-4-hydroxy-5-formamidopyrimidine) [c]: FAPydGMP + H2O ⇒ DR5P + FAPyGN oligopeptide (Val) transport via ABC system ATP[c] + H2O[c] + ValVal[e] ⇒ ADP[c] + H[c] + PI[c] + ValVal[c] tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER oligopeptide (Gly) transport via ABC system ATP[c] + GlyGly[e] + H2O[c] ⇒ ADP[c] + GlyGly[c] + H[c] + PI[c] putrescine transport via ABC system ATP[c] + H2O[c] + PTRC[e] ⇒ ADP[c] + H[c] + PI[c] + PTRC[c] Peptidyl-tRNA hydrolysis (LEU) [c]: AMP_LEU + H2O ⇒ AMP + LEU tRNA U8 sulfurtransferase [c]: ATP + CYS + H2O + UMP ⇒ AMP + H + PPI + s4UMP + SER N(6)-lipoyl-lysine hydrolysis [c]: H2O + LIPOYLLYS ⇒ LIPOATE + LYS 5'-nucleotidase (2'-deoxythymidine monophosphate-[6,4]-2'-deoxycytidine monophosphate) [c]: dTMP64dCMP + (2) H2O ⇒ dT64dC + (2) PI Triacylglycerol lipase (methyl_ocdcea) (MG_327) [e]: H2O + METHYL_OCDCEA ⇔ CH3OH + H + OCDCEA Peptidyl-tRNA hydrolysis (GLY) [c]: AMP_GLY + H2O ⇒ AMP + GLY 2-aminoethylphosphonate transport via ABC system AEPP[e] + ATP[c] + H2O[c] ⇒ ADP[c] + AEPP[c] + H[c] + PI[c] oligopeptide (Met) transport via ABC system ATP[c] + H2O[c] + MetMet[e] ⇒ ADP[c] + H[c] + MetMet[c] + PI[c] Dipeptide import (Tyr-Tyr) [c]: H2O + TyrTyr ⇒ (2) TYR DNA cleavage (dAMP) [c]: dApdAp + H2O ⇒ (2) DAMP + H Manganese-transporting ATPase ATP[c] + H2O[c] + MN[e] ⇒ ADP[c] + H[c] + MN[c] + PI[c] sulfate transporting ATPase ATP[c] + H2O[c] + SO4[e] ⇒ ADP[c] + H[c] + PI[c] + SO4[c] DNA base glucosyl transfer (5'-hydroxymethylcytosine ==> beta-D-glucosylhydroxymethylcytosine; glucose) [c]: GLC + hm5CSN ⇒ glchm5CSN + H2O oligopeptide (Pro) transport via ABC system ATP[c] + H2O[c] + ProPro[e] ⇒ ADP[c] + H[c] + PI[c] + ProPro[c] toxin secretion (nisin) via ABC system ATP[c] + H2O[c] + nisin[c] ⇒ ADP[c] + H[c] + nisin[e] + PI[c] Protein polymerization (Asp) [c]: (2) ASP ⇒ AspAsp + H2O

Comments
Comments	pH 7.5 is equal to a concentration of 3.16e-5 mM. However the simulation suggests additional hydrogen is required for growth. Concentration set to 0.5 mM to support growth.

Metadata
Created	2012-10-01 15:07:00
Last updated	2012-10-01 15:12:14


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