GlyA3 – glycine hydroxymethyltransferase, reversible

Name
WID	GlyA3
Name	glycine hydroxymethyltransferase, reversible
Cross references	EC: 2.1.2.1, BiGG: GHMT2r, BioCyc: GLYOHMETRANS-RXN, KEGG: R00945

Classification
Type	chemical

Reaction
Stoichiometry	[c]: (2) H + SER + THF ⇒ GLY + H2O + METTHF

Catalysis
Enzyme	MG_394_TETRAMER [c]
Coenzymes	PL5P [c] (Show evidence) Value: PL5P Measured: Yes References Chang A, Scheer M, Grote A, Schomburg I, Schomburg D. BRENDA, AMENDA and FRENDA the enzyme information system: new content and tools in 2009. Nucleic Acids Res 37, D588-92 (2009). WholeCell: PUB_0570, PubMed: 18984617 Value: PL5P Measured: Yes Species: Escherichia coli K12 References Keseler IM, Bonavides-Martínez C, Collado-Vides J, Gama-Castro S, Gunsalus RP, Johnson DA, Krummenacker M, Nolan LM, Paley S, Paulsen IT, Peralta-Gil M, Santos-Zavaleta A, Shearer AG, Karp PD. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res 37, D464-70 (2009). WholeCell: PUB_0006, PubMed: 18974181 Value: PL5P Measured: Yes Species: Escherichia coli K-12 MG1655 References UniProt Consortium. The Universal Protein Resource (UniProt) 2009. Nucleic Acids Res 37, D169-74 (2009). WholeCell: PUB_0096, PubMed: 18836194, URL: http://www.uniprot.org/uniprot/
Optimal pH	7.6 (dimensionless)
Optimal temperature	49.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	0.100
K_eq	0.84696481244 ()

Kinetics
Forward kinetics	v = V_max * [SER] * [THF] / (K_m1 * K_m2 + K_m1 * [SER] + K_m2 * [THF]); K_m1 = 80 (nM), K_m2 = 150 (nM), V_max = 13.6 U/mg (Show evidence) Value: Km = ALLO-THR 1500,D-ALANINE 30000,GLY 250,THF 25,SER 300,L-threonine 12000 (None) Measured: Yes Species: Escherichia coli References Keseler IM, Bonavides-Martínez C, Collado-Vides J, Gama-Castro S, Gunsalus RP, Johnson DA, Krummenacker M, Nolan LM, Paley S, Paulsen IT, Peralta-Gil M, Santos-Zavaleta A, Shearer AG, Karp PD. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res 37, D464-70 (2009). WholeCell: PUB_0006, PubMed: 18974181 Value: Rate law: VmaxSERTHF/(Km1Km2+Km1SER+Km2THF); Km = 0.08 mM, 0.15 mM, Vmax = 13.6 umol/(minmg) (None) Measured: Yes Species: Escherichia coli References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Process	Process_Metabolism

Comments
References	Chang A, Scheer M, Grote A, Schomburg I, Schomburg D. BRENDA, AMENDA and FRENDA the enzyme information system: new content and tools in 2009. Nucleic Acids Res 37, D588-92 (2009). WholeCell: PUB_0570, PubMed: 18984617 Keseler IM, Bonavides-Martínez C, Collado-Vides J, Gama-Castro S, Gunsalus RP, Johnson DA, Krummenacker M, Nolan LM, Paley S, Paulsen IT, Peralta-Gil M, Santos-Zavaleta A, Shearer AG, Karp PD. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res 37, D464-70 (2009). WholeCell: PUB_0006, PubMed: 18974181 UniProt Consortium. The Universal Protein Resource (UniProt) 2009. Nucleic Acids Res 37, D169-74 (2009). WholeCell: PUB_0096, PubMed: 18836194, URL: http://www.uniprot.org/uniprot/ Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:35
Last updated	2012-10-01 15:16:35