FruK1 – fructose-1-phosphate kinase

Name
WID	FruK1
Name	fructose-1-phosphate kinase
Cross references	BioCyc: 1PFRUCTPHOSN-RXN, EC: 2.7.1.56, BiGG: FRUK, KEGG: R02071

Classification
Type	chemical

Reaction
Stoichiometry	[c]: ATP + F1P ⇒ ADP + FDP + H

Catalysis
Enzyme	MG_063_DIMER [c]
Optimal pH	7.8 (dimensionless)
Optimal temperature	30.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	-3.80
K_eq	550.954774449 ()

Kinetics
Forward kinetics	v = V_max * [ATP] * [F1P] / (K_m1 * K_m2 + K_m1 * [ATP] + K_m2 * [F1P]); K_m1 = 750 (nM), K_m2 = 700 (nM), V_max = 47.0 U/mg (Show evidence) Value: Km = FRU1P 360,ATP 350,FRUCTOSE-6P 3500 (None) Measured: Yes Species: Escherichia coli References Keseler IM, Bonavides-Martínez C, Collado-Vides J, Gama-Castro S, Gunsalus RP, Johnson DA, Krummenacker M, Nolan LM, Paley S, Paulsen IT, Peralta-Gil M, Santos-Zavaleta A, Shearer AG, Karp PD. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res 37, D464-70 (2009). WholeCell: PUB_0006, PubMed: 18974181 Value: Rate law: VmaxATPF1P/(Km1Km2+Km1ATP+Km2F1P); Km = 0.75 mM, 0.7 mM, Vmax = 47 umol/(minmg) (None) Measured: Yes Species: Aerobacter aerogenes References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Pathways	ALT_CARBON_METABOLISM
Process	Process_Metabolism

Comments
References	Keseler IM, Bonavides-Martínez C, Collado-Vides J, Gama-Castro S, Gunsalus RP, Johnson DA, Krummenacker M, Nolan LM, Paley S, Paulsen IT, Peralta-Gil M, Santos-Zavaleta A, Shearer AG, Karp PD. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res 37, D464-70 (2009). WholeCell: PUB_0006, PubMed: 18974181 Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:34
Last updated	2012-10-01 15:16:31