Fre5 – FMN reductase

Name
WID	Fre5
Name	FMN reductase
Cross references	EC: 1.5.1.38, SABIO-RK: 14263, BiGG: FMNRx

Classification
Type	chemical

Reaction
Stoichiometry	[c]: FMN + H + NADH ⇒ FMNH2 + NAD

Catalysis
Enzyme	MG_342_MONOMER [c]
Optimal pH	7.9 (dimensionless)
Optimal temperature	40.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	-3.70
K_eq	466.639382275 ()

Kinetics
Forward kinetics	v = V_max * [NADH] / (K_m + [NADH]); K_m = 5.4 (nM), V_max = 1680.0 1/min (Show evidence) Value: Km = NADPH 5.4,FMN 1.600e-2 (None) Measured: Yes Species: Escherichia coli References Keseler IM, Bonavides-Martínez C, Collado-Vides J, Gama-Castro S, Gunsalus RP, Johnson DA, Krummenacker M, Nolan LM, Paley S, Paulsen IT, Peralta-Gil M, Santos-Zavaleta A, Shearer AG, Karp PD. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res 37, D464-70 (2009). WholeCell: PUB_0006, PubMed: 18974181 Value: Rate law: VmaxNADH/(Km+NADH); Km = 5.4 uM, Vmax = 28 1/sec (None) Measured: Yes* Species: Paracoccus denitrificans References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Pathways	COFACTOR_PROSTHETIC_BIOSYNTHESIS
Process	Process_Metabolism

Comments
References	Keseler IM, Bonavides-Martínez C, Collado-Vides J, Gama-Castro S, Gunsalus RP, Johnson DA, Krummenacker M, Nolan LM, Paley S, Paulsen IT, Peralta-Gil M, Santos-Zavaleta A, Shearer AG, Karp PD. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res 37, D464-70 (2009). WholeCell: PUB_0006, PubMed: 18974181 Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:34
Last updated	2012-10-01 15:16:31