FolD1 – methylenetetrahydrofolate dehydrogenase (NADP)

Name
WID	FolD1
Name	methylenetetrahydrofolate dehydrogenase (NADP)
Cross references	EC: 1.5.1.5, BioCyc: METHYLENETHFDEHYDROG-NADP-RXN, BiGG: MTHFD, KEGG: R01220

Classification
Type	chemical

Reaction
Stoichiometry	[c]: METTHF + NADP ⇔ (2) H + METHF + NADPH

Catalysis
Enzyme	MG_013_DIMER [c]
Optimal pH	7.1 (dimensionless)
Optimal temperature	47.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	2.00
K_eq	0.0360833936403 ()

Kinetics
Forward kinetics	v = V_max * [METTHF] * [NADP] / (K_m1 * K_m2 + K_m2 * [METTHF] + K_m1 * [NADP]); K_m1 = 92 (nM), K_m2 = 45 (nM), V_max = 160.0 U/mg (Show evidence) Value: Rate law: VmaxMETTHFNADP/(Km1Km2+Km2METTHF+Km1NADP); Km = 0.092 mM,0.045 mM, Vmax = 160 umol/(minmg) (None) Measured: Yes Species: Salmonella typhimurium References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Pathways	FOLATE_METABOLISM
Process	Process_Metabolism

Comments
Comments	KM, METTHF=92 μM, KNADP 45 μM, and Vmax=160 U/mg were observed in Salmonella typhimurium [PUB_0100].
References	Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:34
Last updated	2012-10-01 15:16:29