FTHFS – formate-tetrahydrofolate ligase

Name
WID FTHFS View in model
Name formate-tetrahydrofolate ligase View in model
Cross references EC: 6.3.4.3, KEGG: R00943
 
Classification
Type chemical View in model
 
Reaction
Stoichiometry [c]: ATP + FOR + THF ⇒ ADP + FTHF10 + PI View in model
 
Catalysis
Enzyme MG_013_DIMER [c] View in model
 
Energetics
Is spontaneous (pH 7.5, 25C, I = 0) False View in model
ΔG (pH 7.5, 25C, I = 0; kJ mol‑1) -11.10
 
Kinetics
Forward kinetics Vmax = 364.8 1/min View in model
 
Associations
Pathways FOLATE_METABOLISM
Process Process_Metabolism View in model
 
Comments
Comments BioCyc annotation notes FolD catalyzes the formate-tetrahydrofolate ligase reaction [PUB_0006]. Pfeiffer et al characterized the elementary flux modes of folate metabolism in M. pneumoniae [PUB_0864]. Leaphart et al observed that the kinetic rate of FolD is 6.08 1/s [PUB_0863].
References

  1. Keseler IM, Bonavides-Martínez C, Collado-Vides J, Gama-Castro S, Gunsalus RP, Johnson DA, Krummenacker M, Nolan LM, Paley S, Paulsen IT, Peralta-Gil M, Santos-Zavaleta A, Shearer AG, Karp PD. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res 37, D464-70 (2009). WholeCell: PUB_0006, PubMed: 18974181

  2. Leaphart AB, Trent Spencer H, Lovell CR. Site-directed mutagenesis of a potential catalytic and formyl phosphate binding site and substrate inhibition of N10-formyltetrahydrofolate synthetase. Arch Biochem Biophys 408, 137-43 (2002). WholeCell: PUB_0863, PubMed: 12485612

  3. Pfeiffer T, Dandekar T, Moldenhauer F, Schuster S. Eds Hofmeyr J-HS, Rohwer, JM, Snoep JL. Topological analysis of metabolic networks. Application to the metabolism of Mycoplasma pneumoniae. University Press 229-234 (2000). WholeCell: PUB_0864, URL: http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.106.6168&rep=rep1&type=pdf
 
Metadata
Created 2012-10-01 15:08:34
Last updated 2012-10-01 15:16:32