Eno – enolase

Name
WID	Eno
Name	enolase
Cross references	BioCyc: 2PGADEHYDRAT-RXN, EC: 4.2.1.11, SABIO-RK: 7357, BiGG: ENO, KEGG: R00658

Classification
Type	chemical

Reaction
Stoichiometry	[c]: G2P ⇔ H2O + PEP

Catalysis
Enzyme	MG_407_DIMER [c]
Optimal pH	8.1 (dimensionless)
Optimal temperature	64.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	-0.900
K_eq	4.4587350229 ()

Kinetics
Forward kinetics	v = V_max * [G2P] / (K_m + [G2P]); K_m = 100 (nM), V_max = 180.0 U/mg (Show evidence) Value: Rate law: VmaxG2P/(Km+G2P); Km = 0.1 mM, Vmax = 180 U/mg (None) Measured: Yes* Species: Escherichia coli References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Pathways	GLYCOLYSIS
Process	Process_Metabolism

Comments
References	Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:33
Last updated	2012-10-01 15:16:28