DeoD4 – purine-nucleoside phosphorylase (Inosine)

Name
WID	DeoD4
Name	purine-nucleoside phosphorylase (Inosine)
Cross references	SABIO-RK: 17689, EC: 2.4.2.1, BioCyc: INOPHOSPHOR-RXN, BiGG: PUNP5, KEGG: R01863

Classification
Type	chemical

Reaction
Stoichiometry	[c]: HYXN + R1P ⇔ (2) H + INS + PI

Catalysis
Enzyme	MG_049_HEXAMER [c]
Optimal pH	7.6 (dimensionless)
Optimal temperature	60.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	2.00
K_eq	0.0360833936403 ()

Kinetics
Forward kinetics	(Show evidence) Value: Km = RIBOSE-1P 40,ADENINE 40,ADENOSINE 46,DEOXY-RIBOSE-1P 100,DEOXYGUANOSINE 98.5,DEOXYINOSINE 106,HYPOXANTHINE 26.6,GUANOSINE 20,Pi 120,INOSINE 96 (None) Measured: Yes Species: Escherichia coli References Keseler IM, Bonavides-Martínez C, Collado-Vides J, Gama-Castro S, Gunsalus RP, Johnson DA, Krummenacker M, Nolan LM, Paley S, Paulsen IT, Peralta-Gil M, Santos-Zavaleta A, Shearer AG, Karp PD. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res 37, D464-70 (2009). WholeCell: PUB_0006, PubMed: 18974181 Value: Rate law: VmaxHYXN/(Km+HYXN); Km = 0.03 mM, Vmax = 110 1/sec (None) Measured: Yes* Species: Homo sapiens References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/ Value: Rate law: VmaxINS/(Km+INS) (None) Measured: Yes* References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/
Backward kinetics	v = V_max * [INS] / (K_m + [INS]); V_max = 0.0141 U/mg

Associations
Pathways	NUCLEOTIDE_SALVAGE_PWY
Process	Process_Metabolism

Comments
References	Keseler IM, Bonavides-Martínez C, Collado-Vides J, Gama-Castro S, Gunsalus RP, Johnson DA, Krummenacker M, Nolan LM, Paley S, Paulsen IT, Peralta-Gil M, Santos-Zavaleta A, Shearer AG, Karp PD. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res 37, D464-70 (2009). WholeCell: PUB_0006, PubMed: 18974181 Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:08
Last updated	2012-10-01 15:15:05