DeoD2 – purine-nucleoside phosphorylase (Deoxyadenosine)

Name
WID	DeoD2
Name	purine-nucleoside phosphorylase (Deoxyadenosine)
Cross references	EC: 2.4.2.1, BiGG: PUNP2, KEGG: R02557

Classification
Type	chemical

Reaction
Stoichiometry	[c]: DA + PI ⇔ AD + DR1P

Catalysis
Enzyme	MG_049_HEXAMER [c]
Optimal pH	7.6 (dimensionless)
Optimal temperature	60.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	2.00
K_eq	0.0360833936403 ()

Kinetics
Forward kinetics	v = V_max * [DA] / (K_m + [DA]); K_m = 145 (nM), V_max = 4.55 U/mg (Show evidence) Value: Rate law: VmaxDA/(Km+DA); Km = 0.145 mM, Vmax = 4.55 umol/(minmg) (None) Measured: Yes Species: Bos taurus References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Pathways	NUCLEOTIDE_SALVAGE_PWY
Process	Process_Metabolism

Comments
References	Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:08
Last updated	2012-10-01 15:15:04