DNA_RM_MunI_Restriction – MunI DNA restriction/modification, DNA restriction

Name
WID DNA_RM_MunI_Restriction View in model
Name MunI DNA restriction/modification, DNA restriction View in model
 
Classification
Type DNA_restriction_modification View in model
 
Reaction
Stoichiometry [c]: (2) dRibose5P_dRibose5P + (2) H2O ⇒ (4) DR5P + (2) H View in model
 
Energetics
Is spontaneous (pH 7.5, 25C, I = 0) False View in model
 
Associations
Process Process_DNARepair View in model
 
Comments
Comments MunI type II restriction/modification restriction reaction. MunI recognizes the sequence 5'-CAATTG-3' [PUB_0096]. MunI methylase methylates the N6 position of A-3 of hemimethylated DNA producing N6-methyladenine, and MunI endonuclease cleaves the C-A phosphodiester bond of unmethylated DNA in an ATP-independent manner [PUB_0006]. In constrant to type I restriction/modification systems, the methylase and endonuclease are two independent proteins. M. genitalium only contains the methylase of the MunI restriction/modification system. Baldwin et al observed that steady-state restriction kinetics vary from 0.072-0.66 1/s as a function of divalent ion concentrations, and is maximal for Mg2+ [PUB_0545]. Horton et al observed a kinetic rate of 36 1/min [PUB_0546]. Kurpiewski et al observed a kinetic rate of 0.49 1/s [PUB_0547].
References

  1. Baldwin GS, Sessions RB, Erskine SG, Halford SE. DNA cleavage by the EcoRV restriction endonuclease: roles of divalent metal ions in specificity and catalysis. J Mol Biol 288, 87-103 (1999). WholeCell: PUB_0545, PubMed: 10329128

  2. Horton NC, Otey C, Lusetti S, Sam MD, Kohn J, Martin AM, Ananthnarayan V, Perona JJ. Electrostatic contributions to site specific DNA cleavage by EcoRV endonuclease. Biochemistry 41, 10754-63 (2002). WholeCell: PUB_0546, PubMed: 12196013

  3. Keseler IM, Bonavides-Martínez C, Collado-Vides J, Gama-Castro S, Gunsalus RP, Johnson DA, Krummenacker M, Nolan LM, Paley S, Paulsen IT, Peralta-Gil M, Santos-Zavaleta A, Shearer AG, Karp PD. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res 37, D464-70 (2009). WholeCell: PUB_0006, PubMed: 18974181

  4. Kurpiewski MR, Engler LE, Wozniak LA, Kobylanska A, Koziolkiewicz M, Stec WJ, Jen-Jacobson L. Mechanisms of coupling between DNA recognition specificity and catalysis in EcoRI endonuclease. Structure 12, 1775-88 (2004). WholeCell: PUB_0547, PubMed: 15458627

  5. UniProt Consortium. The Universal Protein Resource (UniProt) 2009. Nucleic Acids Res 37, D169-74 (2009). WholeCell: PUB_0096, PubMed: 18836194, URL: http://www.uniprot.org/uniprot/
 
Metadata
Created 2012-10-01 15:08:09
Last updated 2012-10-01 15:15:07