DNA_POLYMERASE_CORE – DNA-directed DNA polymerase

Name DNA-directed DNA polymerase View in model
Cross references PDB: 3BEP, PDB: 1OK7
Biosynthesis [c]: MG_031_MONOMER + MG_261_MONOMERDNA_POLYMERASE_CORE View in model
No. subunits 2
DNA footprint Length: 24 (nt), Binding: ssDNA, Region: xsDNA View in model
Empirical formula (pH 7.5) H19170C12218N3146O3494S60
Molecular weight (pH 7.5; Da) 267959.06 View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
Half life (OD (600 nm) = 0.3, 
M9 media, 36C; min)
1200.00 View in model
Formation process Process_MacromolecularComplexation View in model
Localization c
Enzyme View in model
Complex subunit View in model
Comments Composition Inferred from B. subtilis DNA pol III holoenzyme structure [PUB_0312, PUB_0313, PUB_0314, PUB_0428]. DNA-binding DNA polymerase III holoenzyme has 27-30 nt footprint [PUB_0743]. base distance between the helicase and the leading polymerase polymerization site. [PUB_0739] Actual gap distance not known, but it is known that the polymerase occupies 24 bases.
  1. Bruck I, O'Donnell M. The DNA replication machine of a gram-positive organism. J Biol Chem 275, 28971-83 (2000). WholeCell: PUB_0314, PubMed: 10878011

  2. Kelman Z, O'Donnell M. DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine. Annu Rev Biochem 64, 171-200 (1995). WholeCell: PUB_0312, PubMed: 7574479

  3. Kurth I, O'Donnell M. Replisome Dynamics during Chromosome Duplication. Chapter 4.4.2 (2009). WholeCell: PUB_0739, URL: http://www.ecosal.org

  4. Razin S, Herrmann R. Molecular biology and pathogenicity of mycoplasmas. Springer (2002). WholeCell: PUB_0428, ISBN: 9780306472879

  5. Razin S, Yogev D, Naot Y. Molecular biology and pathogenicity of mycoplasmas. Microbiol Mol Biol Rev 62, 1094-156 (1998). WholeCell: PUB_0313, PubMed: 9841667

  6. Reems JA, McHenry CS. Escherichia coli DNA polymerase III holoenzyme footprints three helical turns of its primer. J Biol Chem 269, 33091-6 (1994). WholeCell: PUB_0743, PubMed: 7806536

Created 2012-10-01 15:07:35
Last updated 2012-10-01 15:14:01