DNADamage_URA_ho5URA_hydroxyl_radical – DNA radiation (gamma-ray) induced base oxidation (uracil ==> 5-hydroxyuracil; hydroxyl radical)

WID DNADamage_URA_ho5URA_hydroxyl_radical View in model
Name DNA radiation (gamma-ray) induced base oxidation (uracil ==> 5-hydroxyuracil; hydroxyl radical) View in model
Type radiation_induced_base_oxidation View in model
Stoichiometry gamma_radiation[e] + hydroxyl_radical[c] + URA[c] ⇒ H[c] + ho5URA[c] View in model
Is spontaneous (pH 7.5, 25C, I = 0) True View in model
Process Process_DNADamage View in model
Comments Exclusively derived from dC [PUB_0473]. Reaction rate computed from 1) total gamma ray base damage yield 1.7e-9 1/Gy/DA [PUB_0497], 2) 305 nt/DA, and 3) relative yields of various damaged bases [PUB_0461, PUB_0501].
  1. Dizdaroglu M. Measurement of radiation-induced damage to DNA at the molecular level. Int J Radiat Biol 61, 175-83 (1992). WholeCell: PUB_0501, PubMed: 1351904

  2. Friedberg EC, Walker GC, Siede W, Wood RD, Schultz RA, Ellenberger T. DNA repair and mutagenesis. ASM Press (2006). WholeCell: PUB_0461, ISBN: 9781555813192

  3. Gulston M, Fulford J, Jenner T, de Lara C, O'Neill P. Clustered DNA damage induced by gamma radiation in human fibroblasts (HF19), hamster (V79-4) cells and plasmid DNA is revealed as Fpg and Nth sensitive sites. Nucleic Acids Res 30, 3464-72 (2002). WholeCell: PUB_0497, PubMed: 12140332

  4. Hatahet Z, Kow YW, Purmal AA, Cunningham RP, Wallace SS. New substrates for old enzymes. 5-Hydroxy-2'-deoxycytidine and 5-hydroxy-2'-deoxyuridine are substrates for Escherichia coli endonuclease III and formamidopyrimidine DNA N-glycosylase, while 5-hydroxy-2'-deoxyuridine is a substrate for uracil DNA N-glycosylase. J Biol Chem 269, 18814-20 (1994). WholeCell: PUB_0473, PubMed: 8034633

Created 2012-10-01 15:08:33
Last updated 2012-10-01 15:16:26