ATPHs_DNASupercoiling_gyrase – Negative supercoiling (gyrase)

Name
WID ATPHs_DNASupercoiling_gyrase View in model
Name Negative supercoiling (gyrase) View in model
 
Reaction
Stoichiometry [c]: (2) ATP + (2) H2O ⇒ (2) ADP + (2) H + (2) PI View in model
 
Catalysis
Enzyme DNA_GYRASE [c] View in model
 
Energetics
Is spontaneous (pH 7.5, 25C, I = 0) False View in model
 
Kinetics
Forward kinetics Vmax = 72.0 1/min View in model
 
Associations
Process Process_DNASupercoiling View in model
 
Comments
Comments Reaction occurs with σsp > -0.1 [PUB_0753] with δLK=-2 [PUB_0695], kcat=1.2 1/s [PUB_0753], and mean dwell time 45 s [PUB_0753].
References

  1. Chen Y, Bjornson K, Redick SD, Erickson HP. A rapid fluorescence assay for FtsZ assembly indicates cooperative assembly with a dimer nucleus. Biophys J 88, 505-14 (2005). WholeCell: PUB_0753, PubMed: 15475583

  2. Nöllmann M, Stone MD, Bryant Z, Gore J, Crisona NJ, Hong SC, Mitelheiser S, Maxwell A, Bustamante C, Cozzarelli NR. Multiple modes of Escherichia coli DNA gyrase activity revealed by force and torque. Nat Struct Mol Biol 14, 264-71 (2007). WholeCell: PUB_0695, PubMed: 17334374
 
Metadata
Created 2012-10-01 15:08:06
Last updated 2012-10-01 15:14:59