TranscriptionalRegulation_hrcA_TU_258 – Transcriptional regulation of TU_258 by hrcA

WID TranscriptionalRegulation_hrcA_TU_258
Name Transcriptional regulation of TU_258 by hrcA
Transcription unit TU_258 View in model
Transcripton factor MG_205_DIMER View in model
Binding site 453291454318 MG_355 MG_356
Chromosome: Mgenitalium_Chr_1, Coordinate: 453791 (nt), Length: 27 (nt), Direction: Reverse, Sequence:
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Fold‑change activity
0.0778 (dimensionless)
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Comments HrcA represses the transcription of the heat-shock proteins including chaperones and chaperonins [PUB_0186, PUB_0418, PUB_0419, PUB_0433]. HrcA recognizes the CIRCE motif (TTAGCACTCAAA[GC][TGC][ACGT]TT[TA]GAGTGCTAA). HrcA transcriptional regulatory activity was measured in M. genitalium [PUB_0186] and M. pneumoniae [PUB_0418]. HrcA is repressed by heat, leading to derepression or increased expression of chaperones and chaperonins [PUB_0186, PUB_0418, PUB_0419, PUB_0433]. However there is no CIRCE motif upstream of this transcription unit [PUB_0186]. Consequently, we set the transcription factor binding site by setting its distance from the start codon to be the mean of that of other HrcA binding sites (61 nt). Binding site has motif CIRCE (TTAGCACTCAAA[GC][TGC][ACGT]TT[TA]GAGTGCTAA). Site responds to the condition: absence of 42C heat.
  1. Güell M, van Noort V, Yus E, Chen WH, Leigh-Bell J, Michalodimitrakis K, Yamada T, Arumugam M, Doerks T, Kühner S, Rode M, Suyama M, Schmidt S, Gavin AC, Bork P, Serrano L. Transcriptome complexity in a genome-reduced bacterium. Science 326, 1268-71 (2009). WholeCell: PUB_0418, PubMed: 19965477

  2. Musatovova O, Dhandayuthapani S, Baseman JB. Transcriptional heat shock response in the smallest known self-replicating cell, Mycoplasma genitalium. J Bacteriol 188, 2845-55 (2006). WholeCell: PUB_0186, PubMed: 16585746

  3. Sierro N, Makita Y, de Hoon M, Nakai K. DBTBS: a database of transcriptional regulation in Bacillus subtilis containing upstream intergenic conservation information. Nucleic Acids Res 36, D93-6 (2008). WholeCell: PUB_0419, PubMed: 17962296, URL:

  4. Zuber U, Schumann W. CIRCE, a novel heat shock element involved in regulation of heat shock operon dnaK of Bacillus subtilis. J Bacteriol 176, 1359-63 (1994). WholeCell: PUB_0433, PubMed: 8113175

Created 2012-10-01 15:09:28
Last updated 2012-10-01 15:18:57