Tmk – thiamine kinase

Name
WID Tmk View in model
Name thiamine kinase View in model
Cross references EC: 2.7.1.89, BioCyc: DTMPKI-RXN, KEGG: R02094, BiGG: TMK
 
Classification
Type chemical View in model
 
Reaction
Stoichiometry [c]: ATP + DTMPADP + DTDP View in model
 
Catalysis
Enzyme MG_006_DIMER [c] View in model
Optimal pH
7.9 (dimensionless)
Optimal temperature
45.0 (C)
 
Energetics
Is spontaneous (pH 7.5, 25C, I = 0) False View in model
ΔG (pH 7.5, 25C, I = 0; kJ mol‑1) -3.80
Keq
550.954774449 ()
 
Kinetics
Forward kinetics
v = Vmax * [DTMP] / (Km + [DTMP]); Km = 4.9 (nM), Vmax = 0.0833 U/mg (Show evidence)
View in model
 
Associations
Pathways COFACTOR_PROSTHETIC_BIOSYNTHESIS
Process Process_Metabolism View in model
 
Comments
Comments Measured Vmax=0.833 U/mg. Vmax set 10X lower to 0.0833 U/mg to fit observed mass doubling time.
References
  1. Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

 
Metadata
Created 2012-10-01 15:09:02
Last updated 2012-10-01 15:17:55