RNA_POLYMERASE_HOLOENZYME – DNA-directed RNA polymerase holoenzyme

Name
WID RNA_POLYMERASE_HOLOENZYME View in model
Name DNA-directed RNA polymerase holoenzyme View in model
 
Structure
Biosynthesis [c]: MG_249_MONOMER + RNA_POLYMERASERNA_POLYMERASE_HOLOENZYME View in model
No. subunits 1
DNA footprint Length: 75 (nt), Binding: ssDNA, Region: dsDNA View in model
Empirical formula (pH 7.5) H32388C20128N5465O6025S102
Molecular weight (pH 7.5; Da) 450608.87 View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
200635.00
Half life (OD (600 nm) = 0.3, 
M9 media, 36C; min)
1200.00 View in model
 
Synthesis
Formation process Process_Transcription View in model
Localization c
 
Function
Reaction participant View in model
Complex subunit DNA-directed RNA polymerase holoenzyme
[c]: MG_249_MONOMER + RNA_POLYMERASERNA_POLYMERASE_HOLOENZYME
View in model
 
Parameters
Parameters View in model
 
Comments
Comments Composition [PUB_0355]. DNA-binding Transcription bubble is 12-17 nt [PUB_0748]. RNA closed complex has 60 nt footprint [PUB_0748]. RNAP open complex has 75 nt footprint [PUB_0748]. Elongating RNAP has 50 nt footprint [PUB_0748].
References
  1. Bordier C, Rossetti GP. Subunit composition of Escherichia coli RNA polymerase during transcription in vitro. Eur J Biochem 65, 147-53 (1976). WholeCell: PUB_0355, PubMed: 776624

  2. Murakami KS, Masuda S, Darst SA. Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 A resolution. Science 296, 1280-4 (2002). WholeCell: PUB_0748, PubMed: 12016306

 
Metadata
Created 2012-10-01 15:07:43
Last updated 2012-10-01 15:14:20