RNA_POLYMERASE – DNA-directed RNA polymerase

Name
WID RNA_POLYMERASE View in model
Name DNA-directed RNA polymerase View in model
 
Structure
Biosynthesis [c]: MG_022_MONOMER + (2) MG_177_MONOMER + MG_340_MONOMER + MG_341_MONOMERRNA_POLYMERASE View in model
No. subunits 5
DNA footprint Length: 75 (nt), Binding: ssDNA, Region: dsDNA View in model
Empirical formula (pH 7.5) H28250C17586N4739O5241S94
Molecular weight (pH 7.5; Da) 392938.06 View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
-233065.00
Half life (OD (600 nm) = 0.3, 
M9 media, 36C; min)
1200.00 View in model
 
Synthesis
Formation process Process_MacromolecularComplexation View in model
Localization c
 
Function
Enzyme View in model
Reaction participant View in model
Complex subunit View in model
 
Parameters
Parameters View in model
 
Comments
Comments From crystal structure of Thermus thermophilus RNA polymerase [PUB_0430]; Gram positive bacteria such as mycoplasmas contain additional delta factor beyond gram negative RNA polymerase [PUB_0423]; omega subunit missing. DNA-binding Transcription bubble is 12-17 nt [PUB_0748]. RNA closed complex has 60 nt footprint [PUB_0748]. RNAP open complex has 75 nt footprint [PUB_0748]. Elongating RNAP has 50 nt footprint [PUB_0748].
References
  1. Achberger EC, Whiteley HR. The role of the delta peptide of the Bacillus subtilis RNA polymerase in promoter selection. J Biol Chem 256, 7424-32 (1981). WholeCell: PUB_0423, PubMed: 6788769

  2. Murakami KS, Masuda S, Darst SA. Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 A resolution. Science 296, 1280-4 (2002). WholeCell: PUB_0748, PubMed: 12016306

  3. Vassylyev DG, Sekine S, Laptenko O, Lee J, Vassylyeva MN, Borukhov S, Yokoyama S. Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 A resolution. Nature 417, 712-9 (2002). WholeCell: PUB_0430, PubMed: 12000971

 
Metadata
Created 2012-10-01 15:07:43
Last updated 2012-10-01 15:14:20